... rate constants of enzyme substrate (or protein–ligand) interactions from rapid reaction kinetic data J Biol Chem 250, 404 8–4 052 Fang J, Sawa T, Akaike T, Akuta T, Sahoo SK, Khaled G, Hamada A ... have proposed the use of d-amino acid oxidase (DAAO) from Rhodotorula gracilis (EC 1.4.3.3) for cancer treatment [11] Subsequently, the strategy for cancer therapy based on oxystress and DAAO ... evolution approach to obtain yeast DAAO variants with substantially increased activity at low O2 and d-amino acid concentrations This could lead to better efficacy in therapeutic applications Results...