... Mopholino targeting at the splice junction of exon and intron of irf6 pre-mRNA (E3I3) in zebrafish that strongly suggests a critical role of Irf6 in proper gastrulation and early embryogenesis In ... profiling microarray analysis of the E3I3 injected embryos Perturbation of Irf6 either by injection of Irf6 mRNA encoding only its DNA-binding domain (Sabel et al., 20...
... Mopholino targeting at the splice junction of exon and intron of irf6 pre-mRNA (E3I3) in zebrafish that strongly suggests a critical role of Irf6 in proper gastrulation and early embryogenesis In ... profiling microarray analysis of the E3I3 injected embryos Perturbation of Irf6 either by injection of Irf6 mRNA encoding only its DNA-binding domain (Sabel et al., 20...
... X-box-regulated /ciliary genes Many, or even the majority, of these candidate ciliary genes when mutated may cause a dye filling defect Since the majority (83 out of 93) of the candidate X-box-regulated genes ... of bona fide Xbox regulated genes in C elegans In fact, there are still seven dyf genes (dyf-4, dyf-7, dyf-8, dyf-9, dyf-10, dyf-11 and dyf12) in C el...
... comparison of miRNA abundance in seeds and other organs of soybeans should uncover those miRNAs specifically expressed in seeds Identification of the corresponding target genes and study of their ... sequencing Methods 2007, 43:110-117 doi:10.1186/1471-2229-11-5 Cite this article as: Song et al.: Identification of miRNAs and their target genes in...
... activity and expression profiles of target genes A number of genes, including those for trefoil factor 1/ pS2, cathepsin D, cyclin D1, c-Myc and progesterone receptor, are positively regulated by ... factor binding elements occur very frequently in the genome, finding an ERE, AP-1 or Sp1 site only in ER-responsive genes is highly unlikely Instead, we asked whether the pr...
... the hindbrain and its deficiency in morphants is in line with expression of these genes in this part of brain ctrl/brain A gli2b MO/brain B ctrl/trunk D gli2 MO/brain C gli2b MO/trunk E gli2 ... that in Gli2b morphant (data now shown) These results indicated a minor role of both gli2 and gli2b in regulating expression of nkx2.2 in the ventral hindbrain Due to d...
... containing gli2 genes 101 Results 3 .2 Expression analysis of gli2b in zebrafish 3 .2. 1 Temporal expression of gli2b in zebrafish embryos Temporal expression of gli2b was first compared with that of ... hpf gli2b dorsal smu-/-/48 hpf B gli2b dorsal syu-/-/48 hpf C gli2b dorsal cyclopamine/48 hpf E gli2b WT/48 hpf D gli2b dorsal shh mRNA/48 hpf F Fig 3-10 gli2b expression...
... that pro and sentences are a mixture of opinions and facts, making identifying them in online reviews a distinct problem from opinion sentence identification Finally, we also apply the resulting ... Determining the Sentiment of Opinions Proceedings of COLING-04 pp 1367-1373 Geneva, Switzerland Kim, Soo-Min and Eduard Hovy 2005 Automatic Detection of Opinion Bearing Words...
... Identification of cytochrome P450 monooxygenase genes from the white-rot fungus Phlebia brevispora Ryoich Nakamura1, Ryuichiro Kondo2, Ming-hao ... than the corresponding intron in pb-3, each type of intron was in the same order in pb-1 and pb-3 On the other hand, pb-2 was quite different from the other two 15 CYP genes in all properties of ... to the p...
... Identification of cytochrome P450 monooxygenase genes from the white-rot fungus Phlebia brevispora Ryoich Nakamura1, Ryuichiro Kondo2, Ming-hao ... than the corresponding intron in pb-3, each type of intron was in the same order in pb-1 and pb-3 On the other hand, pb-2 was quite different from the other two 15 CYP genes in all properties of ... to the phy...
... (5'TG AAAACTGAACAAAAGAC3') together with one of the reverse primers S1OLIGO3' (CTAAACTAACATAAGGG C3') or degenerate3'-2 (5'CCATAAGTAACATAAGGG CAA3') [14,16] The RT reaction for synthesis of cDNA ... isolates using the QIAamp MinElute Virus Spin Kit (QIAGEN, USA) Amplification of the S1 gene by RT-PCR was performed using one of the forward primers S1OLIGO5' (5'TGA AAACTGA...
... capable of detecting coding motifs functional at the DNA level MicroRNA binding motifs Finally, we considered whether COMIT was able to detect microRNA binding sites in coding regions We first examined ... explains the activity of diverse experimentally tested motifs Exonic splicing enhancers To verify the efficacy of our algorithms, we examined the sequence conservation of...
... of the endocardium and erythrocytes in zebrafish embryos resulting in defects in heart- looping, valve formation, and cardiac output The induction of apoptosis of the endocardium and erythrocytes ... detected the presence of ROS in erythrocytes and DNA fragmentation in both the endocardium and erythrocytes of b-lapachone- treated emb...