Development and application of solvent minimized extraction technologies

... Effect of extraction solvent volume on extraction efficiency Figure 6-5 Effect of type of dispersive solvent and demusification solvent Figure 6-6 Effect of volume of dispersive solvent and demusification ... selection of extraction solvent 124 6.3.2.2 The volume of the extraction solvent 125 6.3.2.3 Selection of dispersive solvent and demulsification solvent 126 6.3.2.4 Volume of the dispersive solvent ... of type of extraction solvent on extraction Figure 4-5 Effect of extraction solvent volume on extraction Figure 4-6 Effect of temperature on extraction Figure 4-7 Extraction time profiles Figure...

Development and application of mass spectrometry based proteomics technologies to decipher ku70 functions

... describes development and application of mass spectrometry based proteomics technologies to decipher Ku70 biological functions We first improve the proteomics sample preparation method in order to enhance ... Silver staining of different amount of BSA picomol, 500 femtomol, 100 femtomol, 50 femtomol, 10 femtomol, femtomol to femtomol BSA (From left to right)were loaded and run by SDS-PAGE and then stained ... Complexity in Eluate Submitting to Mass Spectrometry 62 3.3.6 Identification of Ku70 Complex by Mass Spectrometry 64 3.3.7 Validation of Search Result of Mass Spectrometry 67 3.3.8 Poly...

Development of solvent minimized extraction procedures for environmental analysis

... analysis) Notable among recent developments are simple, faster and greener (environmentally friendly) microextraction techniques This thesis focuses on the developments of solvent- minimized extraction ... Supercritical fluid extraction (SFE) is also a very popular technique for environmental analysis It is an appropriate technique for the analysis of the less volatile compounds, much like solvent extraction ... the use of reduced organic solvent and better automation with modern instruments have led to recent developments of miniaturized liquid-liquid extractions procedures 1.2.2 Flow Injection Analysis...

the development and application of an antibody based biosensor

... spectrum of BT5AA 56 Synthesis and mass spectrum of 2TAA 58 Synthesis and mass spectrum of 3TAA 59 Synthesis and mass spectrum of 5M2TAA 60 Synthesis and mass spectrum of ... from the ancient remains of marine plant and animal life under extreme heat and pressure in an anaerobic environment Depending on the composition of the organic material, varying proportions of ... Schematic of a generalized biosensor The bioreceptor and the transducer are linked such that the transducer quantifies the biorecognition event occurring between the sample and the bioreceptor The...

Báo cáo hóa học: " Development and application of a biomarker assay for determining the pharmacodynamic activity of an antagonist candidate biotherapeutic antibody to IL21R in whole blood" pptx

... biomarkers, and they participated in the data analyses AAH developed the customized Spotfire tool used for data analyses and reviewed statistical analyses YG participated in the assessment and ... assay for determining the pharmacodynamic activity of an antagonist candidate biotherapeutic antibody to IL21R in whole blood Journal of Translational Medicine 2010, 8:51 Page 13 of 13 ... this basic approach (ex vivo stimulation of whole blood) to develop pharmacodynamic biomarker assays for a candidate therapeutic antibody, Ab-01 Ab-01, a human antibody generated by phage display,...

Báo cáo y học: "Development and application of versatile high density microarrays for genome-wide analysis of Streptomyces coelicolor: characterization of the HspR regulon" ppsx

... (and Lys) tend to be over- High density IJISS DNA arrays have been developed for global analysis of Streptomyces gene expression and transcription factor binding The HspR regulatory system of S ... against the test arrays and the probe performance was scored using the following equations Firstly the Cy3 and Cy5 background-subtracted signals, designated 'g' and 'r', respectively, obtained by feature ... chaperone and protease production It is envisaged that these arrays will find widespread use in systems level analysis of Streptomyces coelicolor biology Materials and methods Streptomyces strains and...

development and application of mixed beam-solid models for analysis of soil-pile interaction problems

...

DEVELOPMENT AND APPLICATION OF NEW APPROACHES FOR STUDIES OF INFLUENZA INDUCED INFLAMMATION AND DNA DAMAGE

... of influenza A (H1N1) virus infection to explore the impact of influenza infection and inflammation on DNA damage and DNA damage responses By studying chromatin phosphorylation as a measure of DNA ... of DNA damage, we show that the level of DNA damage increases following influenza infection, and we provide data that supports a role for replication fork breakdown as a driver of DNA strand breaks ... Together, these studies raise the possibility that DNA damage and DNA repair modulate the severity of influenza -induced cytotoxicity, thereby affecting tissue damage and regeneration, and ultimately...

Development and application of a high throughput assay for discovery of starch hydrolase inhibitors

... αglucosidase and α-amylase can significantly reduce the post-prandial increase in blood glucose after a meal, with starch as the major calorie intake The apparent advantage of the starch hydrolase inhibitors ... linear range of gelatinized starch Table Accuracy and precision of the high- throughput method Table IC50 and acarbose equivalence of inhibitors obtained by starch hydrolases Table Estimation of ... of starch involves saliva α-amylase, pancreatic αamylase, and the small intestinal brush border α-glucosidase, that is, maltaseamyloglucosidase and sucrase-isomaltase [19, 67] Determination of...

Development and application of computational methods and tools for adverse drug reaction and toxicity prediction

... develop and apply computational methods and tools for ADR and toxicity prediction The methods developed in this work are potentially useful for development and application of QSAR models as well ... complex, time consuming and resource intensive Toxicity is one of the primary reasons for the failure of drug candidates in later stages of drug development Moreover, adverse drug reaction (ADR) during ... drug development and safety assessment 1.2 QSAR studies for ADR and toxicity prediction To deliver promising drug candidates to reach the late stage of drug development with a higher chance of...

Part 1 development and application of hantzsch ester and hypervalent iodine reagent

... irradiated at 14 0 oC for 40 Figure 2 -1 Library of 2 -1- 16, 2 -1- 17, 2 -1- 18, 2 -1- 19, 2 -1- 20 32 2.2.2.3 Solid-Phase Synthesis of 2 -1- 16, 2 -1- 18, 2 -1- 19 and 2 -1- 20 To illustrate the versatility of this ... process 10 Figure 1- 8 Safety-catch Linkers 12 Figure 2 -1 Library of 2 -1- 16, 2 -1- 17, 2 -1- 18, 2 -1- 19, 2 -1- 20 31 Figure 3 -1 Polymer-supported Hantzsch ester 56 Figure 3-2 Reduction of Ketimines and ... Part 1: COMBINATORIAL SYNTHESIS OF NITROGENCONTAINING HETEROCYCLES Part 2A: DEVELOPMENT AND APPLICATION OF HANTZSCH ESTER Part 2B: DEVELOPMENT AND APPLICATION OF A HYPERVALENT IODINE REAGENT...

Development and application of bioinformatics tools for discovery disease markers and disease targeting antibodies

... differentiation, and to the design of disease- targeting molecules like small-molecule agents or antibodies for disease treatment The key disease markers determine the characteristics of disease, therefore ... effectiveness of our system on deriving stable disease markers and discovering therapeutic target One major application of marker discovery is the finding of disease targeting molecules for disease ... the beginning of Chapter two since this method is used in both disease marker discovery and disease targeting molecule design The strategies for disease marker discovery and disease targeting molecule...

Development and application of hollow fiber protected liquid phase microextraction for trace organic analysis

... Three -phase hollow fiber- protected liquid- phase microextraction techniques combined with HPLC analysis 42 2.1 Orthogonal array designs for the optimization of static three -phase hollow fiber- protected ... the hollow fiber This thesis reports on the development and application of hollow fiber- protected LPME techniques to trace organic analysis Chapter provides an introduction to extraction, and ... pores of hollow fiber, and the aqueous acceptor solution in the lumen of the hollow fiber was repeatedly moved in and out of the hollow fiber and the syringe Compared to the dynamic three-phase...

Development and application of hybrid finite methods for solution of time dependent maxwells equations

... domain finite methods for the numerical solution of time dependent Maxwell’s equations The two finite methods focused are FDTD and FETD methods The applications of the developed hybrid methods are ... analysis of passive microwave circuits and studies in dosimetry and tissue interaction CHAPTER TIME DOMAIN FINITE METHODS FOR SOLUTION OF MAXWELL’S EQUATIONS 2.1 Maxwell’s Equations The physics of time ... equivalence of FDTD and a particular case of FETD method a stable hybrid 3-D FETD-FDTD method was proposed The focus of this thesis is in the development and subsequent applications of efficient hybrid time...

Development and application of novel capillary electrophoresis techniques for analysis of DNA fragments and organic pollutants

... focused on the analysis of DNA fragments; and the second part (chapter and chapter 5) focused on the analysis of organic pollutants Several novel capillary electrophoresis (CE) techniques had ... various applications, the CE applications in DNA analysis and monitoring of pollutants are of significant importance 1.3.1 CE Application in DNA Analysis DNA separation by CE has been the subject of ... for on-site analysis of various pollutants at trace level CE is found to be a versatile analytical tool for the analysis of DNA as well as pollutants Combination of DNA analysis and environmental...

Xem thêm