Cytophysiologic effects and molecular inhibition of a functional actin specific ADP ribosyltransferase CDT from clostridium difficile 1
Cytophysiologic effects and molecular inhibition of a functional actin specific ADP ribosyltransferase CDT from clostridium difficile 1
... 3 .10 Protein profiles and autoradiograms showing ADP- ribosylated actin by various CDTa isoforms and NAD photolabeled CDTa 81 3 .11 Proposed mechanism of ADP- ribosylation of actin by ADPRT ... antibiotic-associated diarrhea ADP adenosine diphosphate ATP adenosine triphosphate ADPRT ADP- ribosyltransferase ARTase ADP- ribosylation assay amp ampicillin bp base pairs BHI brain heart infusion CIAP calf ... expression patterns would be useful for both research and clinical applications Using ADP- ribosyltransferase assay (ARTase), analyses of wild-type and mutant CDTa activities revealed conserved amino acid...
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Cytophysiologic effects and molecular inhibition of a functional actin specific ADP ribosyltransferase CDT from clostridium difficile 5
... Lachumanan, R., Armugam, A. , Durairaj, P., Gopalakrishnakone, P., Tan, C H., and Jeyaseelan, K (1999) In situ hybridization and immunohistochemical analysis of the expression of cardiotoxin and ... C., and Benyamin, Y (2004) Biochemical characterization of the L-plastin -actin interaction shows a resemblance with that of alpha-actinin and allows a distinction to be made between the two actin- binding ... Song, K P (2004) Comparative and quantitative transcription analyses of the binary ADP- ribosyltransferase cdt from a variant Clostridium difficile strain with truncated pathogenicity locus J...
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Cytophysiologic effects and molecular inhibition of a functional actin specific ADP ribosyltransferase CDT from clostridium difficile 4
... Rho activation to allow actin nucleation/polymerization associated with Rac and Cdc42 activities This is reflective of the fact that immediate establishment of focal adhesion and spreading was ... heterogeneity Instead, we 133 focused on the functional properties of both CDTa and CDTb and their effects on cellular physiology CDTa exhibited modification of actin monomer that caused dramatic F -actin ... beneficial applications, CDTa as an actin- freezing molecule and inducer of cAMP, MAPKs and other components of signal transduction pathways, is a promising pharmacologic agent that can be utilized...
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Cytophysiologic effects and molecular inhibition of a functional actin specific ADP ribosyltransferase CDT from clostridium difficile 3
... CAGTTGTTATTTTGTACTGACATATCATATAAATACATATTTT -117 -35 -10 TATGATATATAGTTACATATTTTATGAAATTTATATAAAAAAT -74 TCTTATTTAGATTATATAATCTAAATAAATTAAAGTTCAAGAG -31 -35 -10 Start cdtA +1 TTAATTAAACTAATATTGGGAGGGAGAATAAATGAAAAAATTT ... TTAATTAAACTAATATTGGGAGGGAGAATAAATGAAAAAATTT 12 AGGAAACAT TGATGCAACATTGA 138 3 Stop TACCTTAA tattttttcacataaataatttaatatttttcaa Start cdtB atttaaggAGGAGAaaca ATGAAAATACAAATGAGGAATAAA 24 Figure 3. 4 Characteristic features of 2 030 9 ... Ia botA Ttgaca* Ttgaca* Ttgaca* TTGTTA TATAAT TTAACA TTTACA TTTACA TTAGCA TTTACA TATGTC TTTACA TGCACA TTGTCAT TTAACC TATAAT TATAAT TAtAAT* TATAAA TTCAAG TTATCT CTCCTT GTCTTT TATAGT TTATTC TATTTT...
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Cytophysiologic effects and molecular inhibition of a functional actin specific ADP ribosyltransferase CDT from clostridium difficile 2
... CTGGAGATTCAAAATAATAGACATAC R 4 42- 417 AF271719 cdb3 GAACTAATAACTCTCTATCGTCTGG R 4061-4037 AF271719 cdb4 TTGTCTTTATCCAGAAGTTTATCTAC R 1 725 -1700 AF271719 cdb7 ATGTTAAACTTGAAAGAGGAATGA F 324 9- 327 2 AF271719 ... cdb8 GGAGATCCAAATCAGCCTAAAAC F 32- 3954 AF271719 Rcda-For GAAGCAGAAAGAATAGAG F 21 7 -23 4 AF271719 Rcda-Rev ATTAGGTAACAAACCCTCA R 1609-1591 AF271719 Rcdb-For GTGGGAAGATAGTTTTGC F 7 32- 749 AF271719 ... 1010-1031 AF271719 CDTaY344-Rev TACAGTTAAATTAGTTGGAATAGGTTCACG R 1017-1000 AF271719 CDTaR345-For CAACTAATTTAACTGTATAT(GC)(CT)AAGATCTGCTCC F 1013-1034 AF271719 CDTaR345-Rev ATATACAGTTAAATTAGTTGGAATAGGTTC...
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Báo cáo khoa học: Functional fine-mapping and molecular modeling of a conserved loop epitope of the measles virus hemagglutinin protein pdf
... bonds stabilizes a conformational epitope of the apical membrane antigen-1 of Plasmodium falciparum [27] Although the sequential epitope of VP1 of foot and mouth disease does not contain intramolecular ... induced against the linear isoform of the HNE peptide Although the binding pattern may be somewhat blurred by these antibodies and by the polyclonal nature of the sera, the importance of residues ... information is available and data about the role of the cysteines are conflicting Hu & Norrby [24] suggested that C381 and C494 participate in unspecified intramolecular Ó FEBS 2003 Fine-mapping of an...
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Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx
... A novel high-activity D-hydantoinase from Jannaschia sp CCS1 obtain optically pure amino acids, namely chemical and enzymatic syntheses Chemical synthesis gives racemic mixtures of amino acids ... precipitate fraction; sup, supernatant fraction The molecular weight standard (lane M) is indicated on the right A novel high-activity D-hydantoinase from Jannaschia sp CCS1 Fig Purification of HYDBp ... high-activity D-hydantoinase from Jannaschia sp CCS1 Y Cai et al Experimental procedures Genome mining and identification of putative D-hydantoinase genes Using the amino acid sequence of HYDBp (AAL37185)...
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Báo cáo khóa học: Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea docx
... although, as V volvacea produces at least one other laccase isoform in addition to lac1 [1], this and possibly other laccase isoforms may be contributing to the total laccase activity detected at the ... primers The putative N-glycosylation site is boxed; *; stop codon The putative polyadenylation signals (TATAAA and CATAAA) are in white on a black background Ó FEBS 2003 Laccase gene from Volvariella ... min; then a final extension at 72 °C for 10 The primers for lac1 PLAC1F (5¢-AGCTTT CATTCCCAGTGATTG-3¢) and PLAC1R (5¢-AACGAG CTCAAGTACAAATGACT-3¢) were designed according to our cloned cDNA (GenBank...
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Tài liệu Báo cáo khoa học: Purification and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx
... Kumagaye KY, Nakajima K, Watanabe T, Kawai T, Kawakami Y, Niidome T, Sawada K, Nishizawa Y et al (1994) Omega-agatoxinTK containing D-serine at position 46, but not synthetic omega-[L-Ser46]agatoxin-TK, ... Inoue A, Kawakami Y, Nishizawa Y, Katayama K & Kuwada M (1995) Isolation and characterization of a peptide isomerase from funnel web spider venom J Biol Chem 270, 16719–16723 Torres AM, Tsampazi ... assigned and integrated, with concomitant cycles of structure calculations for evaluation of distance and angle constraint violations as well as assignments of additional peaks based on the preliminary...
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Báo cáo khoa học: Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes pdf
... were made with PYMOL [47] able sequences, some bacterial family 19 chitinases have catalytic domains that are at least as large as those of the plant enzymes and that may contain at least six ... 35 Kawase T, Yokokawa S, Saito A, Fujii T, Nikaidou N, Miyashita K & Watanabe T (2006) Comparison of enzymatic and antifungal properties between family 18 and 19 chitinases from S coelicolor A3 (2) ... Haemophilus influenzae, and Pseudomonas aeruginosa Although many plant family 19 chitinases are known, crystal structures are available for only two of these [9,15–17] The first structure of a bacterial...
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Báo cáo Y học: Purification and catalytic properties of a CO-oxidizing:H2-evolving enzyme complex from Carboxydothermus hydrogenoformans doc
... Fe-containing catalytic subunit of CO dehydrogenase A catalytically active CooSI dimer has been previously purified and characterized from C hydrogenoformans [25] The 89- and 62-kDa polypeptides were assigned ... enzyme complex from Carboxydothermus hydrogenoformans After cell breakage and separation of the membrane fraction from the soluble fraction, 80–90% of the hydrogenase activity and 60–70% of the ... hydrogenoformans composed of a hydrogenase and a CO dehydrogenases The complex catalyzes the conversion of CO to CO2 and H2 MATERIALS AND METHODS Materials Dodecyl-b-D-maltoside was from Glycon...
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Báo cáo y học: " Mixed infection and clonal representativeness of a single sputum sample in tuberculosis patients from a " docx
... infection Mixed infection Mixed infection Mixed infection Mixed infection Mixed infection Mixed infection Mixed infection Mixed infection Mixed infection Mixed infection Mixed infection Mixed infection ... infection Mixed infection Mixed infection Mixed infection Mixed infection Mixed infection Mixed infection Mixed infection Mixed infection Mixed infection Mixed infection Mixed infection Mixed infection ... contamination during sample preparation for MIRU-VNTR typing, sample preparation for PCR, and the addition of DNA was done in a laminar flow cabinet The H37Rv M tuberculosis strain and water...
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Sea piracy and the formation of a regional response constructing asean maritime security from the strait of malacca anti piracy cooperation
... AMF ASEAN Maritime Forum eAMF Expanded ASEAN Maritime Forum AMMTC ASEAN Ministerial Meetings on Transnational Crime APSC ASEAN Political -Security Community APT ASEAN plus Three (ASEAN +3) ARF ASEAN ... China Sea In addition, sea piracy in Southeast Asian waters remains a modern-day issue, hardly an antiquated concern of the past The timeliness and magnitude of sea piracy in the Strait of Malacca ... prominently on ASEAN s security agenda and ASEAN- related fora However, it can be claimed that should the maritime piracy concerns in the Strait of Malacca have been resolved via ASEAN- affiliated security...
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Báo cáo khoa học: Molecular cloning and functional expression of a gene encoding an antiarrhythmia peptide derived from the scorpion toxin pptx
... E.P., Martin-Eauclaire, M.F., Mansuelle, P & Sampieri, F (1991) An anti-insect toxin purified from the scorpion Androctonus australis Hector also acts on the alpha- and beta-sites of the mammalian ... Sampieri, F & Granier, C (1991) An anti-insect toxin purified from the scorpion Androctonus australis Hector also acts on the a- and b-sites of the mammalian sodium channel: sequence and circular dichroism ... obtained was displayed an ORF of 258 bp encoding a polypeptide of 85 amino acids and termed BmKIM The 5¢ and 3¢ UTRs of BmKIM cDNA are 17 bp and 76 bp, respectively A single AATAAA polyadenylation...
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Báo cáo y học: "Survivors of war in the Northern Kosovo (II): baseline clinical and functional assessment and lasting effects on the health of a vulnerable population" ppsx
... Survivors of war in the Northern Kosovo (II): baseline clinical and functional assessment and lasting effects on the health of a vulnerable population Conflict and Health 2010 4:16 Submit your next manuscript ... physical functioning and activity, participation in social life and environmental factors was obtained in further interviews using a questionnaire based on the WHO International Classification ... Vergara A, Agani F, Gotway CA: Mental health, social functioning, and attitudes of Kosovar Albanians following the war in Kosovo Jama 2000, 284(5):569-577 13 American Association for the Advancement...
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Từ khóa: molecular mechanisms biological effects and therapeutic strategies of protectionfunctional and molecular aspects of glucocorticoids in the endocrine pancreas and glucose homeosphysiology and molecular biology of salinity stress tolerance in plantsrocky and the eye of a tigercalculate the molar mass and molecular formula of the solidmolar mass and chemical formula of a volatile liquidlist of input output and storage devices of a computerwork on your own initiative and as part of a teamthe subject and the object of a sentencedefine vapor pressure and boiling point of a liquidhow to write a materials and methods section of a research paperdesign and development stages of a productdefine the terms vapor pressure and boiling point of a liquidwhat is the empirical formula molar mass and molecular formula of ch4ngeometric dimensioning and tolerancing profile of a surfaceBáo cáo quy trình mua hàng CT CP Công Nghệ NPVNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếNghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngThơ nôm tứ tuyệt trào phúng hồ xuân hươngSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXTổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)Kiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Quản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIChiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015Đổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ