Development of hybrid promoters and viral vectors for improved gene delivery to the central nervous system

... overview of the progress made by using different types of promoters and vectors for gene delivery to the CNS 1.1 Current progress in gene therapy Gene therapy, which usually refers to somatic gene therapy, ... by the selection of promoters The gene delivery vector that carries and transfers the gene into the nuclei of target cell is another key Chapter Introduction issue of gene therapy The tropism of ... 2003) 1.4.2 Viral vectors Viral vectors are the most efficient vectors to transduce mammalian cells, and therefore are employed in most gene delivery experiments A variety of viral vectors, including...

Genetic engineering and surface modification of baculovirus derived vectors for improved gene delivery to the central nervous system

... GENETIC ENGINEERING AND SURFACE MODIFICATION OF BACULOVIRUS DERIVED VECTORS FOR IMPROVED GENE DELIVERY TO THE CENTRAL NERVOUS SYSTEM YANG YI (B Eng.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR ... Chapter Three: Genetic Engineering of Baculovirus Vectors for Controlled Gene Delivery ………65 3.1 Introduction………………………………………………………………… 66 3.1.1 Genetic Engineering of Baculovirus Vectors for Neuron-targeted ... SUMMARY The studies presented in this thesis focused on development and modification of baculovirus- based vectors in order to achieve improved gene delivery to the Central Nervous System There...

Design of viral vectors for improved gene delivery

... Packaging of amplicon viral vectors 3.3.10 Analysis of cell cycle-dependent transgene expression in pC8-36 amplicon viral vectors 3.3.11 Effect of transduction of viral vector on the cell cycle profile ... (Castro et al., 2003a) 1.3.2 Gene therapy of gliomas One of the formidable tasks of intracranial gene delivery is the difficulty in achieving gene delivery to > % of the tumor mass It would be ... LIST OF TABLES Table 1.1 Vectors and delivery systems for gene therapy 12 Table 5.1 Determination of transduction efficiency of amplicon viruses in vivo 160 Table 5.2 Comparison of percentage of...

Báo cáo khoa học: "Genomic variability in Potato virus M and the development of RT-PCR and RFLP procedures for the detection of this virus in seed potatoes" ppt

... doi:10.1186/1743-422X-7-25 Cite this article as: Xu et al.: Genomic variability in Potato virus M and the development of RT-PCR and RFLP procedures for the detection of this virus in seed potatoes Virology ... detected in several potato samples from an experimental farm in the province of Page of Figure Sensitivity of RT-PCR using primer set PVM3/PVM4 for detecting PVM in composite sprout (A) and dormant ... tested in RT-PCR using this primer set to determine specificity None of them yielded any amplification products To evaluate the sensitivity of the RT-PCR for the detection of PVM in dormant tubers...

RESEARCH ON THE GROWTH AND DEVELOPMENT OF TARO VARIETIES AND CULTIVATION TECHNIQUES FOR THE POTENTIAL VARIETY IN YEN BAI PROVINCE

... activities of study and evaluating the growth and development of some taro varieties on single-crop-land and lowland in Yen Bai province, finding out the most potential and suitable variety for the ... implemented the study titled "Research on the growth and development of taro varieties and cultivation techniques for the potential variety in Yen Bai province" 2 The purpose of study Through the activities ... 1: Studying the effect of climatic and land conditions to develop taro plant production in Yen Bai province - Content 2: Studying the growth and development of several taro varieties on single-crop-land...

Báo cáo hóa học: " Research Article Binocular Image Sequence Analysis: Integration of Stereo Disparity and Optic Flow for Improved Obstacle Detection and Tracking" pptx

... calculate the optic flows for the left and right image sequences 2.1 Optic flows of moving obstacles Many approaches have been designed for obtaining optic flows for moving objects from image sequences ... “Cooperative integration of stereopsis and optical flow computation,” Journal of the Optical Society of America A, vol 12, no 12, pp 2564–2572, 1995 [14] S P Clifford and N M Nasrabadi, Integration of stereo ... INTEGRATING OPTIC FLOWS WITH STEREO MATCHING For a stereovision-based object detection system, two images (left and right) sequences can be simultaneously acquired This enables acquisition of a pair of...

báo cáo khoa học:" Development of a patient reported outcome scale for fatigue in multiple sclerosis: The Neurological Fatigue Index (NFI-MS)" pptx

... phase of measurement, with the aim of developing a valid and reliable patient reported outcome scale for fatigue, the Neurological Fatigue Index (NFIMS) The items in the scale are based on the ... the notion that the sub-dimensions were part of a single, supraordinate theme of neurological fatigue Fit of scale data to the Rasch model also allows for a transformation of the ordinal raw ... Trust, for allowing the approach of patients under their care; and Dave Watling and the staff of the Clinical Trials Unit, WCNN for their assistance with the mailout Author details The Walton...

An investigation into students’ conversational needs and some suggestions for a speaking syllabus to the 2nd year english bachel

... designing a syllabus are illustrated as follows Needs analysis-Objectives and aims-Sequencing–Teaching method–Testing and evaluation As a result, analyzing the needs of learners is the first and the foremost ... able to learn the foreign language as a means for close communication and acceptance by people who speak it Learners with instrumental motivation may learn a foreign language for an intermediate ... official and fixed syllabus; on the other hand, the current syllabus seems not satisfied with the students’ conversational needs Thus, analyzing the students’ needs to design an appropriate speaking...

báo cáo hóa học: " Temporal expression and cellular origin of CC chemokine receptors CCR1, CCR2 and CCR5 in the central nervous system: insight into mechanisms of MOG-induced EAE" pptx

... cells in defined lesional stages Quantification of CCR1, CCR2 and CCR5 mRNA expressing Quantification of CCR1, CCR2 and CCR5 mRNA expressing cells in defined lesional stages Mean numbers of CCR1+ ... expression of CCR1, CCR2 and CCR5 in the spinal cord (data not shown) Enhanced expression of CCR1, CCR2 and CCR5 mRNA was subsequently observed over cells within inflammatory aggregates during the early ... of CCR1, CCR2 and CCR5 mRNA in the rat spinal cord No expression of CCR1, CCR2 or CCR5 was detected within the spinal cord of healthy control rats (Fig 2A, 2D, 2G) or MOG-EAE rats in the pre-symptomatic...

Báo cáo khoa học: "Immunohistochemical study of constitutive neuronal and inducible nitric oxide synthase in the central nervous system of goat with natural listeriosis" potx

... counterstained with hematoxylin Magnification A and B, ×66 C and D, ×132 Immunohistochmical study of nitric oxide synthase in the central nervous system of goat Enhanced expression of nNOS and iNOS in ... is the first study of NOS expression in the goat brain with listeriosis The involvement of iNOS in bovine and caprine listeriosis has been reported previously, and the increased expression of iNOS ... microabscesses, and some astrocytes (identical in Fig Histology and immunostaining of caprine brains with natural listeriosis A: Brain of a goat with listeriosis showing meningoencephalitis H-E staining,...

báo cáo khoa học: "Isolated angiitis of the central nervous system with tumor-like lesion, mimicking brain malignant glioma: a case report and review of the literature" pdf

... Primary angiitis of the central nervous system: serial MRI of brain and spinal cord Neuroradiology 2001, 43:599-607 14 Moore PM: Diagnosis and management of isolated angiitis of the central nervous ... Neuropsychopharmacol Biol Psychiatry 2005, 29:627-631 Carandang C, Grant A: Delirium and Isolated Angiitis of the Central Nervous System: a Case Report and Review CNS Spectrums 2008, 13:209-213 Page of ... treatment protocols used in these cases Fountain et al [23] reported a case with IACNS controlled by cyclophosphamide alone, while Carandang C and Grant A reported a female patient with IACNS...

williams - uncontrolled risk; the lessons of lehman brothers and how systemic risk can still bring down the world financial system (2010)

... UNCONTROLLED RISK THE LESSONS OF LEHMAN BROTHERS AND HOW SYSTEMIC RISK CAN STILL BRING DOWN THE WORLD FINANCIAL SYSTEM M A R K T W I L L I A M S New York ... the prosperity of H Lehman 10 ᭿ Uncontrolled Risk The arrival of Mayer reunited all the brothers In acknowledgment of Mayer’s arrival in 1850, the name of the family business was changed to Lehman ... economy The boom -and- bust cycles of America’s economy, and, indeed, the prosperity of banks have tracked the economic growth of the nation During these cycles, legislation and the level of state...

Bio nanoparticles and bio microfibers for improved gene transfer into glioma cells

... BIO- NANOPARTICLES AND BIO- MICROFIBERS FOR IMPROVED GENE TRANSFER INTO GLIOMA CELLS YANG JINGYE (B Eng.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY NUS GRADUATE SCHOOL FOR INTEGRATIVE ... procedures to form bio- nanoparticles and bio- microfibers The findings in this study should enrich the development of gene therapies for CNS diseases, particularly in glioma tumors, and should advance ... tumor cells The research conducted for this thesis focused mainly on strategic development of gene transfer vectors with the objective of boosting gene delivery performance in glioma cells and...

Báo cáo sinh học: "The long term effects of chemotherapy on the central nervous system" docx

... vulnerable, population of patients Chemotherapy- induced cognitive decline in the absence of radiation The best data on the cognitive effects of chemotherapy alone have come from studies of children with ... high doses of chemotherapy is clearly demonstrated by Noble and colleagues [1], yet these proposed studies not take into consideration the possible effects of high-dose chemotherapy on either CNS ... are planned that further reduce the dose of craniospinal radiation to 1,800 cGy, virtually no attention has been paid to the possible contribution of the chemotherapy to the cognitive decline...

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