Characterization of the function of tight junction proteins in transgenic mice

... drawing of the TJ proteins TJ proteins consist of TM proteins and plaque proteins that link TM proteins to the cytoskeleton (Johnson LG 2005) 16 TM proteins of TJ The three most common TM proteins ... cytoskeleton proteins Some scaffolding TJ proteins lacking PDZ domains such as cingulin can also link integral proteins to the actin cytoskeleton, whereas other function in vesicular trafficking and other ... TJ proteins In TJs, transmembrane (TM) proteins that interact with corresponding proteins on the adjacent membranes are tethered to the actin cytoskeleton via scaffolding proteins (Figure 3) The...

Báo cáo khoa học: Proteomic characterization of lipid raft proteins in amyotrophic lateral sclerosis mouse spinal cord pot

... same mouse; and (b) the protein was consistently identified in the independent Fig Evaluation of the isolation of lipid raft proteins Lipid raft proteins were isolated from spinal cords of symptomatic ... Metabolism AATM _MOUSE CHP1 _MOUSE CD81 _MOUSE CDC42 _MOUSE CAH2 _MOUSE DHPR _MOUSE ALDOC _MOUSE LDHB _MOUSE MDHM _MOUSE MBP _MOUSE MYP0 _MOUSE SIRT2 _MOUSE NFL _MOUSE NPTN _MOUSE PRDX5 _MOUSE MPCP _MOUSE PGAM1 _MOUSE ... Table Proteins uniquely identified in the lipid rafts isolated from WT and G93A mouse spinal cords Accession number Protein name Proteins uniquely identified in the lipid rafts isolated from G93A mouse...

Báo cáo Y học: Detailed characterization of polydnavirus immunoevasive proteins in an endoparasitoid wasp doc

... respectively, instead of the calyx cells Further, the expression of IEP proteins is not synchronized with that of CkPDV capsid proteins; IEP proteins were observed approximately one day after the initiation ... that capsid proteins of CkPDV are present in the calyx cells (Fig 5A) To examine whether IEPs are also synthesized in the calyx cells, immunocytochemical and in situ hybridization analyses were ... weight of approximately 50 kDa may serve as an immunoevasive mediator, and thereby named it immunoevasive protein (IEP) In the present study, we conﬁrmed the immunoevasive activity of IEP for wasp...

Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx

... from the oyster Crassostrea gigas [5] Interestingly, this protein, named C gigas chitinase-like protein (CgClp1) was found to be involved in the control of growth and remodelling processes in a ... composed of the sole Glyco_18 domain, the C-terminal tail of C gigas CLPs may not noticeably contribute to the structure and the function of these proteins Interestingly, Cg-Clp1 and Cg-Clp2 C-terminal ... based on the conserved amino acid sequences of the catalytic domain of members of the GH18 family resulted in the ampliﬁcation of an expected 147 bp sequence Cloning and sequencing of this fragment...

Báo cáo y học: "Early Characterization of Toll-like receptors in primary lung epithelial cells: strong impact of the TLR3 ligand poly(I:C) on the regulation of Toll-like receptors, adaptor proteins and inflammatory response" ppt

... is in accordance with the highly increased cytokine and chemokine secretion induced by poly(I:C) and emphasizes the strong proinflammatory impact of the TLR3 ligand poly(I:C) for SAEC Regulation ... expression of other TLRs and proteins involved in TLR signaling in SAEC In addition, we demonstrate that the expression of TLRs and their signaling proteins in SAEC is strongly regulated by type-1 and ... release of inflammatory mediators The family of Toll-like receptors (TLR) plays a key role in pathogen recognition and induction and regulation of the innate and adaptive immune response Airway epithelial...

Báo cáo khoa học: Structural and functional characterization of human Iba proteins ppt

... cross-linking efciency of Iba1 and Iba2 or in the overall morphology of the generated lament bundles Calcium afnity of Iba1 and Iba2 Homodimerization and actin binding of Iba1 and Iba2 were similar ... presented here reveals functional similarities and differences between Iba1 and Iba2 We investigated Ca2+ binding and homodimerization of Iba1 and Iba2 Furthermore, F-actin binding and cross-linking ... Human Iba proteins J O Schulze et al study has revealed expression proles for most of the human transcripts and uncovered different tissuespecic expression of Iba1 and Iba2 [5] For Iba1 ,...

Báo cáo khóa học: Characterization of novel structural features in the lipopolysaccharide of nondisease associated nontypeable Haemophilus inﬂuenzae pptx

... have been identiﬁed in those strains that are responsible for most of the steps in the biosynthesis of the oligosaccharide portions of the LPS molecules The inner core of H in uenzae LPS has been ... common structural feature on surface structures of pathogens residing in the human respiratory tract, including H in uenzae Expression of PCho has been associated with persistence of H in uenzae in ... within H in uenzae LPS remains to be elucidated Ester-linked glycine has been shown to be a prominent substituent in the core region of H in uenzae LPS All strains investigated so far express minor...

Characterization of Spin Coated Polymers in Nano-environments as a Function of Film Thickness pot

... Characterization of Spin Coated Polymers in Nano-environments as a Function of Film Thickness (Abstract) Catherine E Beck Polymer applications have become more demanding as industry continuously ... “accelerated to a desired rotation rate.”16 Spinning continued until an equilibrium film thickness was reached This was followed by annealing to alleviate radial orientation and eliminate any remaining solvent ... contact angle of water was measured as a control on a clean (as defined in experimental section) silicon substrate and was 36.0° The contact angle of water was determined for the polymer brush samples...

Báo cáo khoa học: Comparative biochemical characterization of nitrile-forming proteins from plants and insects that alter myrosinase-catalysed hydrolysis of glucosinolatesk docx

... M Burow et al Nitrile-forming proteins from plants and insects Table Biochemical characteristics of ESP The effects of variable temperature, phosphate ions, radical scavengers, and reducing agents ... thaliana ESP and P rapae NSP alter the outcome of myrosinase-catalysed glucosinolate hydrolysis, this comparative biochemical characterization revealed a number of differences between these two proteins, ... Burow et al Nitrile-forming proteins from plants and insects S Glc S O S SO3- O SO3- S Glc O N ial ESPs were originally described as 35–40 kDa proteins that promote the formation of epithionitriles,...

báo cáo khoa học: " Isolation and functional characterization of CE1 binding proteins" pptx

... proteins CEBFs (CE1 binding factors) DNA -binding and transcriptional activities of CEBFs Binding of a number of CEBFs, which were isolated as multiple isolates (Table 1), to the CE1 element was ... Lee et al.: Isolation and functional characterization of CE1 binding proteins BMC Plant Biology 2010 10:277 Submit your next manuscript to BioMed Central and take full advantage of: • Convenient ... 1000 1200 -galactosidase activity Figure Binding and transcriptional activities of CEBFs (A) Binding of CEBFs to the CE1 element The binding activity of six CEBFs was confirmed in yeast Reporter...

báo cáo khoa học: " Exploiting EST databases for the development and characterization of EST-SSR markers in castor bean (Ricinus communis L.)" pptx

... frequency and distribution of SSRs in castor bean ESTs, (2) the establishment and validation of EST- SSR markers for detection of polymorphism in castor bean, and (3) the assessment of genetic ... an increasing need for distinguishing the varieties reliably, establishing their purity, and fingerprinting released varieties, hybrids and the parental lines of castor bean germplasm held in ... doubt, the polymorphic EST- SSR markers developed herein will provide robust genetic markers for further investigation of the origin and evolution of castor bean, though the geographic structuring of...

báo cáo khoa học: " The Puf family of RNA-binding proteins in plants: phylogeny, structural modeling, activity and subcellular localization" potx

... analysis of the amino acids located in the amino-terminal extensions that lie outside of the PUM-HD in AtPum proteins A comparison of the percentage of amino acid identity and similarity for the amino ... resulting in an amino acid triplet that matches that of Puf repeats and in the conserved proteins Interestingly, this CRQ triplet is also found in repeat in some fungal and protozoan Puf proteins ... 1000 nM and 2000 nM samples, indicating that the low affinity binding of the AtPum-HD to the RNA resulted in a dissociation of the complex during electrophoresis Thus, the instability of the complex...

Báo cáo y học: "Characterization of monocyte/macrophage subsets in the skin and peripheral blood derived from patients with systemic sclerosis" pps

... Immunoperoxidase staining of skin in SSc patients compared with that in healthy controls Skin sections from (a to c) healthy controls and (d to f) systemic sclerosis (SSc) patients were stained with anti-human-CD68, ... no link between CD163+ or CD204+ monocyte/macrophage lineage and SSc has been established in the skin or in the peripheral blood of SSc patients Therefore, to more fully explore the nature of ... cells in circulating monocytes of SSc patients by flow cytometric analysis We suggest that CD163+ or CD204+ macrophages in the skin and the CD14brightCD163+CD204+ monocyte subset in the peripheral...

Báo cáo y học: " Role of TNF-α in lung tight junction alteration in mouse model of acute lung inflammation" doc

... 54 55 induced by carrageenan in the mouse model of pleurisy Cytokine 2001, 13(3):162-168 Utsunomiya I, Ito M, Oh-ishi S: Generation of inflammatory cytokines in zymosan-induced pleurisy in rats: ... key step in the pathogenesis of acute lung injury may be myosin light chain kinase activation by TNFα, leading to epithelial barrier dysfunction There is extensive literature examining the consequences ... pulmonary TJ function in vivo, we analyzed the levels of this pro-inflammatory cytokine in TNF-αR1KO and WT mice A substantial increase of TNF-α production was found in pleural exudates and in the lung...

Báo cáo y học: "Phenotypical and functional characterization of alveolar macrophage subpopulations in the lungs of NO2-exposed rats" pot

... subpopulations in NO2-exposed animals The occurrence of phenotypically different AM subpopulations may either be explained by a functional shift of already present AM or by the infiltration of macrophages ... [39,40] In conclusion, our findings clearly suggest that the newly recruited ED7+ AM are involved in the regulation of the ongoing inflammatory process Whether the antiinflammatory effects of IL10 ... Germany) The number of living cells was determined using the CASY®1 Cell Counting System (Schärfe Systems, Reutlingen, Germany) and AMs were incubated at a final concentration of × 106 cells/ml in...

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