... iron- and zinc-containing alcohol dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus J Bacteriol 181, 1163–1170 12 Shimizu Y, Sakuraba H, Kawakami R, Goda S, Kawarabayasi Y ... Journal compilation ª 2006 FEBS R Machielsen and J van der Oost 14 Higashi N, Matsuura T, Nakagawa A & Ishikawa K (2005) Crystallization and preliminary X-ray analysis of hyperthermophilic l-threonine ... reaction The activity of Pf-TDH was significantly increased by the addition of mm CoCl2 (relative activity to that of the standard reaction 170%) and not by the addition of mm ZnCl2 or one of the...