Blastocystis investigations on host pathogen interactions using in vitro model systems
Blastocystis investigations on host pathogen interactions using in vitro model systems
... preparation 4.2.3 Experimental planning and inoculation protocol 4.2.4 DAPI staining for nuclear fragmentation and condensation 4.2.5 Annexin V binding assay for expression of phosphatidylserine molecules ... and concentrations Table 3.1 Percentage of secretory IgA intact heavy chain remaining 75 after incubation with the lysates and conditioned medium of Blastocystis Table 3.2 Degrees of i...
Pathobiological studies of zoonotic blastocystis subtypes using in vitro model systems
... illustrating prevention of Blastocystis- induced ZO-1 and actin rearrangement in Caco-2 monolayers by ROCK and HMG CoA reductase inhibition 151 Fig 5.6 Quantification of ZO-1 staining in Blatocystis-infected ... kinase inhibition 5.3.3 Blastoycstis induces Rho kinase-mediated phosphorylation of Myosin Light Chain at ser-19 position 5.3.4 HMG CoA-reductase inhibition in Caco-2 pre...
Báo cáo y học: " First Dominique Dormont international conference on "Host-pathogen interactions in chronic infections – viral and host determinants of HCV, HCMV, and HIV infections" pdf
... Retrovirology 2005, 2:24 tions" In December 2004, the topic of the first conference was "Viral and Host Determinants of HCV, HCMV and HIV Infections" , considering the pathogenesis of these chronic viral ... response to IL15 was demonstrated only in HIV infection, in terms of survival and cytolysis HCV, HCMV and monocyte activation Assessment of...
Báo cáo sinh học: " In-vitro model systems to study Hepatitis C Virus" ppt
... models currently used to study future HCV research as well as drug design HCV Infection System In the past, research into HCV has been hampered due to lack of a reliable cell culture system Conventional ... month and HCV core protein and HCV negative-strand RNA also detected in infective cells Viral replication had some cytotoxic effects on the cells due to production of interferon as...
Chapter 113. Introduction to Infectious Diseases: Host–Pathogen Interactions (Part 1) docx
... pandemic Many infectious agents have been discovered only in recent decades (Fig 113 -1) Ebola virus, human metapneumovirus, Anaplasma phagocytophila (the agent of human granulocytotropic ehrlichiosis), ... unknown cause, such as rheumatoid arthritis, sarcoidosis, or inflammatory bowel disease, have infectious etiologies There is even evidence that atherosclerosis may have an infectious...
Chapter 113. Introduction to Infectious Diseases: Host–Pathogen Interactions (Part 2) docx
... Chronic pulmonary secretions infection with P aeruginosa INFLAMMATORY RESPONSE Neutropenia Hematologic malignancy, Gram-negative cytotoxic enteric bacilli, chemotherapy, aplastic anemia, Pseudomonas ... infections, gingivitis Phagocytosis (cellular) Systemic lupus Streptococcus erythematosus (SLE), chronic pneumoniae, myelogenous H leukemia, influenzae megaloblastic anemia Splenectomy — H inf...
Chapter 113. Introduction to Infectious Diseases: Host–Pathogen Interactions (Part 4) docx
... transcriptional activator nuclear factor κB (NF-κB), to produce cytokines that lead to inflammation and enzymes that enhance the clearance of microbes These initial responses serve not only to limit infection ... host's own major histocompatibility complex antigen to the T cell receptor Activated T cells may then act in several ways to fight infection Cytotoxic T cells may directly...
Chapter 113. Introduction to Infectious Diseases: Host–Pathogen Interactions (Part 5) doc
... receptors, called selectins (CD62, ELAM-1), in response to inflammatory cytokines such as tumor necrosis factor α and interleukin The binding of these selectin molecules to specific receptors ... bartonellosis, Escherichia coli O157 infection, or cryptococcosis Blood transfusions have been linked to diseases ranging from viral hepatitis to malaria to prion disease A history of expos...
Chapter 113. Introduction to Infectious Diseases: Host–Pathogen Interactions (Part 6) docx
... should be adhered to, and it should be remembered that all antimicrobial agents carry a risk (and a cost) to the patient Direct toxicity may be encountered—e.g., ototoxicity due to aminoglycosides, ... encountered—e.g., ototoxicity due to aminoglycosides, lipodystrophy due to antiretroviral agents, and hepatotoxicity due to antituberculous agents such as isoniazid and rifampin Alle...
Báo cáo sinh học: "No better time to FRET: shedding light on host pathogen interaction" docx
... and phosphatidylinositol-3,4,5triphosphate [PI(3,4,5)P3] Rac1 activation was monitored similarly to that described above To monitor activation of PI-3-kinase, the investigators co-expressed YFP ... 8:772-779 doi:10.1186/jbiol225 Cite this article as: Hayward RD et al No better time to FRET: shedding light on host pathogen interactions Journal of Biology 2010, 9:12 ... py...
Báo cáo lâm nghiệp: "Investigations on vitality and genetic structure in oak stands" pps
... ’European oak decline’ and the influences of decline on genetic structure of stands in the next generation Preconditions for that are investigations concerning the state of damage of oak trees and ... among individuals in heterogeneous environmental conditions From the view of gene conservation and forest tree breeding, it is important to gain information about the geneti...
Investigations on the roles of ubiquitin in the regulation of heat shock gene HSP70B 1
... INVESTIGATIONS ON THE ROLES OF UBIQUITIN IN THE REGULATION OF HEAT SHOCK GENE HSP70B EE THIAM HEE, GARY NATIONAL UNIVERSITY OF SINGAPORE 2 011 INVESTIGATIONS ON THE ROLES OF UBIQUITIN IN THE ... THE REGULATION OF HEAT SHOCK GENE HSP70B EE THIAM HEE, GARY (B SCI (HONS), NUS A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY...
Investigations on the roles of ubiquitin in the regulation of heat shock gene HSP70B 2
... regulation of transcription In this project we investigated the role of ubiquitin in the regulation of the heat shock gene HSP70B Through RNA interference of SKP1, a component of an ubiquitin ligase ... Role of Med21/hSrb7 in the Regulation of HSP70B 109 3.7 The Effect of the RNA Interference of Med21/hSrb7 and its Effects on Proteosomal...
Investigations on the roles of ubiquitin in the regulation of heat shock gene HSP70B 3
... as the link between the methylation of histone H3 residues lysine 34 and lysine 79 (H3K34 and H3K79) and the ubiquitination of histone H2B, abrogating the methylation of H3 while leaving the ubiquitinated ... 2005) 1 .3. 4 E4 Ubiquitin Ligases in the Elongation of Polyubiquitin Chains While the E3 ubiquitin ligases mediate the initial ubiquitination of the s...
báo cáo hóa học:" The effect of newer anti-rheumatic drugs on osteogenic cell proliferation: an in-vitro study" doc
... light of these many uncertainties, we carried out an invitro study of the effect of various antirheumatoid drugs alone and in combination on the proliferation of osteogenic cells derived from the ... proliferation In addition, we found that the effects of these drugs on cell proliferation can vary between donors Although on average Dexamethasone increased...
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