Transplantation of mesenchymal stem cells for the treatment of parkinsons disease in a mouse model

... TRANSPLANTATION OF MESENCHYMAL STEM CELLS FOR THE TREATMENT OF PARKINSON’S DISEASE IN A MOUSE MODEL CHAO YIN XIA MD A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF ANATOMY ... MPTP, an acute insult initiates an inflammatory reaction that becomes self sustaining after the initiating agent has disappeared The MPTP model of PD has been invaluable in the studying of the ... cell transplantation attenuates blood brain barrier damage and neuroinflammation and protects dopaminergic neurons against MPTP toxicity in the substantia nigra in a model of Parkinson’s disease...

Gene expression changes in the brainstem and prefrontal cortex in a mouse model of orofacial pain

... microarray-based approaches to examine gene expression and miRNA changes in the brainstem and prefrontal cortex in a mouse facial carrageenan injection model of orofacial pain At the brainstem level, increased ... Inflammatory pain Tissue injury initiates an inflammatory response that induces pain This type of pain is known as inflammatory pain Inflammatory pain is due mainly to the action of prostaglandins ... demonstrated the involvement of both brainstem and prefrontal cortex in pain, in a mouse model of orofacial pain The differentially expressed genes in different region of the brain i.e brainstem and...

Gene targeting in human pluripotent cell derived neural stem cells for the study and treatment of neurological disorders

... source of cells and the potential to derive the cell type of interest together with the possibility to enrich for genetic modifications during the dividing stem cell state 1.1.1 Human pluripotent stem ... stem cells Landmark discoveries of the young field of human stem cell science were the isolation and culture of inner cell mass from human blastocysts by Bongso in 1994 and in the derivation of the ... in human neuroepithelial-like stem cells for the generation of modified neuronal cultures 59 4.2 Generation of gene- corrected neural stem cells from MJD patient -derived iPS cells...

Báo cáo sinh học: " Effects of transplantation with bone marrowderived mesenchymal stem cells modified by Survivin on experimental stroke in rats" potx

... article as: Liu et al.: Effects of transplantation with bone marrow-derived mesenchymal stem cells modified by Survivin on experimental stroke in rats Journal of Translational Medicine 2011 9:105 Submit ... result in better therapeutic effects for stroke in rats In this paper, we try to investigate the effects of transplantation with MSCs modified by SVV on an experimental stroke model performed in ... adipocyte induction media cells showed adipocyte globules on oil red ‘O’ staining (D) Osteogenic differentiation of MSCs: Upon induction with osteogenic induction media cells showed calcium deposits on...

o cáo hóa học:" Effects of transplantation with bone marrowderived mesenchymal stem cells modified by Survivin on experimental stroke in rats" docx

... differentiation of MSCs: Upon induction with adipocyte induction media cells showed adipocyte globules on oil red O staining (D) Osteogenic differentiation of MSCs: Upon induction with osteogenic induction ... http://www.translational-medicine.com/content/9/1/105 Evaluation of neurological function Evaluation of neurological function was performed day and 14 days after transplantation in each groups (n = 6) using a modified ... harvested bone marrow Bone marrow cells were introduced into 100-mm dishes and cultured in complete medium, consisting of Dulbecco’s Modified Eagle’s Medium (DMEM; Sigma) containing 10% fetal bovine...

Characterization of adult human bone marrow mesenchymal stem cells for effective myocardial repair

... 1.5.2.1 Bone marrow stem cells There are most common types of stem cells in bone marrow: (i) hematopoietic stem cells and (ii) mesenchymal stem cells These cell types are believed to be ideal for myocardial ... Stem cells 1.5.1.1 Embryonic stem cells 1.5.1.2 ES cell stem therapy is not ideal for clinical use 1.5.2 Adult stem cells 10 1.5.2.1 Bone marrow stem cells ... the traditional dogma of adult stem cell commitment and presents the impetus for a burgeoning interest in adult stem cell therapeutics The use of adult bone marrow stem cells in cellular transplantation...

Báo cáo y học: "Adipose-derived mesenchymal stem cells from the sand rat: transforming growth factor beta and 3D co-culture with human disc cells stimulate proteoglycan and collagen type I rich extracellular matrix" ppsx

... antibodies used in 3D immunohistological and CD marker studies Antibody Source Dilution Type I collagen Biodesign International (Kennebunk, ME) 20 μg/ml Type II collagen Biodesign International (Kennebunk, ... dimensional; CD, cluster of differentiation plastic adherence and lineage specific differentiation satisfy the standard criteria suggested for defining mesenchymal stem cells Mesenchymal stem cells ... of transforming in matrix formed by sand rat adipose-derived mesenchymal extracellular 3D factor in the Immunohistochemical documentation ofstem cellsgrowthculture beta sand rat adipose-derived...

Báo cáo y học: " Over-expression of HO-1 on mesenchymal stem cells promotes angiogenesis and improves myocardial function in infarcted myocardium" pdf

... daily intraperitoneal injection of the HO-1 inhibitor zinc-protoporphyrin (ZnPP, Porphyrin Products, Logan, UT, USA) at a concentration of 50 μmol/kg/day, starting two days before and continuing ... et al.: Over-expression of HO-1 on mesenchymal stem cells promotes angiogenesis and improves myocardial function in infarcted myocardium Journal of Biomedical Science 2010 17:80 Submit your next ... contains a cytomegalovirus promoter and a polyadenylation signal of bovine growth hormone For construction of adenovirus containing green fluorescent protein (GFP), a shuttle vector containing...

Tài liệu Báo cáo khoa học: "THE REPRESENTATION OF INCONSISTENT INFORMATION IN A DYNAMIC MODEL-THEORETIC SEMANTICS" ppt

... with a table that contains each ~-expression and the ima6e of its denotation in the current stage of the dynamic model When the domain of the ~-expression expands, the correct denotational relationship ... have the model contain the inconsistent information that "John and Mary talk" is true and that "Mary and John talk" is false If two non-equivalent ~-expressions were identified as being equivalent, ... III Dynamic models contain incomplete information, and the sets, relations, and functions in these models can be incompletely specified (their domains are usually incomplete) In PTQ, some phrases...

Tài liệu Analysing different technology pathways for the pulp and paper industry in a European energy systems perspective doc

... kraft pulp mill based on data from a model mill depicting a typical Scandinavian pulp mill As an addition, Papers IV, VI and VIII are based on data from existing European pulp and paper mills and ... pulp and paper industry Model Paper III Research theme The papers are based on data for a typical Scandinavian kraft pulp mill of today Methodology and model Paper VII Methodology and model Paper ... through gasification Further, a market kraft pulp mill (producing only pulp, not paper) , having a lower steam demand than an integrated kraft pulp and paper mill (producing both pulp and paper) , can...

Báo cáo khoa học: Differential expression of liver and kidney proteins in a mouse model for primary hyperoxaluria type I pdf

... [7], although kinetic studies [8] indicate that its alanineglyoxylate aminotransferase activity is not favored over aminobutyrate-pyruvate, b-alanine-pyruvate and dimethylarginine-pyruvate aminotransferase ... (p97) cofactor (p47) Aminoacylase Phosphoglycerate mutase Indolethylamine N-methyltransferase Peroxiredoxin Apolipoprotein A- I Abhydrolase domain containing 14b D-Amino Protein name ApoA4 ActB ... version 5.0 software (GeneBio, Geneva, Switzerland), including spot detection, quantiﬁcation, normalization, data analysis and statistics Comparative analysis of protein spots was performed by matching...

Báo cáo y học: "Gender-based reciprocal expression of transforming growth factor-β1 and the inducible nitric oxide synthase in a rat model of cyclophosphamide-induced cystitis" ppsx

... TGF-β1 in the urine of male rats after CYP were also significantly higher than the levels of active and total TGF-β1 in the urine of female rats, both at baseline and after CYP (ANOVA, Tukey post ... control rats collected at the same time point of the day The levels of NO2-/NO3- in the urine of CYP-treated female rats remained higher as compared to both CYP-treated and control male rats (ANOVA, ... (panel A) was maintained across all groups The substantial levels of latent TGF-β1 in female rats at baseline and after CYP was accompanied by only minor levels of active TGF-β1 in bladder tissue...

Báo cáo y học: "New insights into the pathogenesis of glucocorticoid-induced avascular necrosis: microarray analysis of gene expression in a rat model" potx

... 10.1186/ar3062 Cite this article as: Kerachian et al., New insights into the pathogenesis of glucocorticoid-induced avascular necrosis: microarray analysis of gene expression in a rat model Arthritis ... vessels of the femoral head In fact, the vascular hypothesis (regional endothelial bed dysfunction) appears to be relevant in the pathogenesis of ANFH Damage or activation of femoral head endothelial ... kit as described by the manufacturer (Affymetrix, Santa Clara, CA, USA) The sense cDNA was then fragmented by uracil DNA glycosylase and Page of 12 apurinic/apyrimidic endonuclease-1 and biotin-labeled...

Báo cáo y học: " Changes in the central component of the hypothalamus-pituitary-thyroid axis in a rabbit model of prolonged critical illness" ppsx

... 5'-GAACAACATGCATTCCGAGAAG-3' Forward: 5'-GCGCAGCGCGTTGAA-3' Probe: 5'-AACGAACAGTCATCACCACATCTCATCCAG-3' Reverse: 5'-GGATGGAGCTCGTCCAAGTG-3' Forward: 5'-GCCATCCTGACTATTTCACTGAAGA-3' Probe: 5'-AAGCCTACTTTTTCTCAAGGGCAGTCACCG-3' ... was therefore used as an internal control Statistical analysis All statistical analyses were done using StatView software (SAS Institute Inc., Cary, NC, USA) Data were analyzed using one-way analysis ... ice and immediately analyzed Protein concentration was measured with the BioRad Protein Assay (Bio-Rad, Veenendaal, The Netherlands) using BSA as the standard following the manufacturer's instructions...

The role of interferon gamma in regulating antigen specific CD8 t cell responses in a mouse model of influenza

... with both the avian and human strains of influenza This facilitates viral reassortment and the generation of novel 10 Chapter 1: Introduction human viral strains with the potential of transferring ... cells HAspecific antibodies form a solid correlate of protection against influenza provided that they match the virus causing the infection In contrast to the antibodies targeted to the HA head region, ... immunity takes days to be initiated and then for the response to peak In the interim, it is the innate immune response that combats viral replication in the initial phase of the infection, buying...

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