Structural studies of glycogen branching enzyme (GLGB) CRFE2alpha urucortin complexes

... STRUCTURAL STUDIES OF GLYCOGEN BRANCHING ENZYME (GLGB) AND CRFR2α:UROCORTIN COMPLEXES KUNTAL PAL (M.Tech Biotech And Biochem Engg.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY ... molecules Inclusion of a branching pattern into the linear form of a polysaccharide is catalyzed by the glycogen branching enzyme (glgB) This transglycosylase enzyme belongs to the family of glycoside ... CRYSTALLOGRAPHY 1.1 STRUCTURAL STUDIES OF MACROMOLECULES Structural studies of macromolecules are relevant to understand their physical and chemical properties This chapter will give an overview of the X-ray...

Synthetic and structural studies of copper, gold and palladium heterocyclic carbene complexes

... ferrocene-derivatized carbene and palladium( I) complexes have been very limited In view of the significance of copper, gold and palladium carbene complexes in organic synthesis, materials and medicine, ... enriching the knowledge of copper, gold and palladium NHC complexes, the results of this research will be instructive for mechanistic study and future designs of copper, gold and palladium NHC catalysts ... However, most of these studies were focused on Pd-, Ni-, Ru- and Rh-NHC complexes NHC complexes of copper and gold have been relatively overlooked in spite of the wide use of copper and gold in catalysis,...

Báo cáo khoa học: Conformational studies of a hyperthermostable enzyme potx

... beam Extreme care was taken to avoid artefacts from depolarization effects At the front of the sample housing a Glan-laser polarizer was mounted, optimizing the already vertical polarization of ... single-domain protein with a molar mass of 30 085 Da Experimental data from mass spectroscopy (MALDI TOF) and size exclusion chromatography showed that LamA in solution is a monomer LamA contains ... from all emitting groups Nevertheless, valuable information can be obtained from analyses of the conformational states of LamA upon heat treatment and in the presence of GdnHCl At the experimental...

Báo cáo khoa học: Structural studies of nucleoside analog and feedback inhibitor binding to Drosophila melanogaster multisubstrate deoxyribonucleoside kinase doc

... structure of dNK has previously been determined in complexes with substrates and a feedback inhibitor [12,13] It has a structure similar to that of the human dGK and dCK and belongs to a structural ... speciﬁcity of dNK Earlier crystallographic studies of substrates dT and dC and on the structure of the feedback inhibitor complex with dTTP, as well as mutation studies, have established some of the ... positioned to form speciﬁc interactions to the sugar and the base of the substrate The 3¢-oxygen of deoxyribose is hydrogen bonded to Tyr70 and Glu172, and the 5¢-oxygen is hydrogen bonded to Glu52 and...

Báo cáo khoa học: Structural studies of thymidine kinases from Bacillus anthracis and Bacillus cereus provide insights into quaternary structure and conformational changes upon substrate binding pot

... this study, we describe the structures of two homologues, TKs from B anthracis and B cereus, in complex with substrate dT and phosphate donormimicking dTTP Because of the high sequence identity ... expression and puriﬁcation of human thymidine kinase 1: quaternary structure, stability, and kinetics Protein Expr Purif 47, 506–515 20 Black ME & Hruby DE (1990) Quaternary structure of vaccinia ... anthracis and B cereus The amino-acid sequence identity between the TKs from B anthracis (Ba-TK) and B cereus (Bc-TK) is as high as 96% The sequence identity with human TK1 (hTK1) is 37–38% From...

Báo cáo khoa học: Structural diversity of angiotensin-converting enzyme Insights from structure–activity comparisons of two Drosophila enzymes docx

... between the two enzymes with a selectivity factor of more than 100 in favour of ACER RXPA380 inhibited ACER with a Ki of 4.8 lm, but did not inhibit ANCE, even at a concentration of 100 lm To ... of the two alanines of RXPA381 pack closely with Val372 of ACER, whereas in ANCE, the methyl group of the terminal alanine residue is orientated away from Thr364, reinforcing the importance of ... of the two active sites of somatic angiotensin-converting enzyme in the cleavage FEBS Journal 273 (2006) 362–373 ª 2005 The Authors Journal compilation ª 2005 FEBS 371 Structure-activity of Drosophila...

Báo cáo khoa học: Structural studies of the capsular polysaccharide and lipopolysaccharide O-antigen of Aeromonas salmonicida strain 80204-1 produced under in vitro and in vivo growth conditions docx

... 271) strain 80204-1 produced under in vitro growth conditions on tryptic soy agar (TSA) and in vivo and have demonstrated that their structures are chemically and antigenically distinct from the ... TOF Analysis of the in vivo cultured A salmonicida strain 80204-1 cells In vivo culture was performed using ligated dialysis tubing bags filled with bacterial suspension of A salmonicida strain ... sample of A salmonicida (data not shown) The proposed structure of CPS and O-chain polysaccharide of A salmonicida strain 80204-1 is depicted in Fig The biological significance of these findings...

Báo cáo Y học: Comparison of starch branching enzyme I and II from potato ppt

... branching enzyme activity on amylose and amylopectin with the starch –iodine assay Amylose (type III, Sigma) and amylopectin (Sigma) from potato were typically dissolved at 10 mg:mL21 in 0.5 M ... required to clarify this and whether the effect of phosphate is of relevance in vivo Branching of linear dextrins To obtain a more detailed comparison of the mode of action of SBE I and SBE II, ... activity of both isoforms increased in the presence of phosphate; (c) GPC results indicate that both SBE I and SBE II mainly branched the linear dextrins used in this study by intrachain branching; ...

biochemical and structural studies of escherichia coli leucine-responsive regulatory protein

... Dissertation Topic: Biochemical and Structural Studies of Escherichia coli Leucine-responsive Regulatory Protein Experience 1997-1998 Teaching Assistant Department of Chemistry University of North Carolina, ... Biochemical and structural studies of Escherichia coli Lrp Transcriptional regulation of gene expression is an essential component for the life of any organism For uropathogenic Escherichia coli, ... this protein to understand more about PapI and how to better work with it 19 Purification and Characterization of Lrp and PapI 2.1 Purification and characterization of Lrp 2.1.1 Materials and...

structural studies of multi-drug resistance p-glycoprotein by electron microscopy

...

Structural studies of cysteine and serine protease inhibitors towards therapeutic applications

... mechanism of cysteine proteases 14 1.3.3 Inhibitors of Cysteine proteases 16 1.3.4 Endogenous inhibitors: Cystatin superfamily 1.3.5 Synthetic inhibitors of Cysteine proteases 1.4 Serine proteases ... mechanism of Serine proteases 23 1.4.2 Serine protease inhibitors 26 1.4.3 Serpins 27 1.4.4 Canonical serine protease inhibitors 29 1.4.5 Non canonical serine protease inhibitors 31 1.4.6 Synthetic inhibitors ... development of the heart and the brain (Hooper et al, 2005) The projects reported in this thesis are mainly related to serine and cysteine proteases and their inhibitors Serine proteases and Cysteine proteases...

Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

... S103 G145/D137 Y 112 D2 01/ 193 L75/67 G145/D137 E1 41/ 133 K173 /16 5 R207 /19 9 G77/69 D142 /13 4 G197 /18 9 Fig Superposition of PRTFDC1 with HPRT-ImmGP (Protein Data Bank code: 1BZY) (A) Residues in the ... Welin et al Studies of the human PRTFDC1 B A PRTFDC1 25 HPRT 20 10 00 0.2 800 600 400 0 .1 200 –50 50 10 10 50 10 015 0 200 250 S 200 –50 250 50 12 00 10 0 15 0 [Hx] (µM) 2.0 PRTFDC1 50 10 0 15 0 200 250 ... 2.0 0 .16 ± 0.02 10 .5 ± 0.9 7.4 · 10 3 ± 1. 9 · 10 3 (0.26%) 2.9 · 10 6 ± 1. 0 · 10 6 (10 0%) 36 .1 ± 14 .3 9.9 ± 0.2 2.9 ± 0.7 899 ± 11 7 1. 36 ± 0.34 406 ± 53 3.9 · 10 4 ± 9.3 · 10 3 (0.09%) 4.5 · 10 7 ± 1. 0...

Tài liệu Báo cáo khoa học: "Predicting the ﬂuency of text with shallow structural features: case studies of machine translation and human-written text" doc

... understand which factors are predictive of good fluency The distribution of fluency scores in the dataset is rather skewed, with the majority of the sentences rated as being of average fluency as ... assess the content of the sentence compared to a human gold-standard Yet, the assessments of the two aspects were often the same—readability /fluency of the sentence is important for understanding the ... from machine translations • Distinguish human and machine translations • Distinguish fluent machine translations from poor machine translations • Distinguish the better (in terms of fluency) translation...

Báo cáo khoa học: NMR and molecular dynamics studies of an autoimmune myelin basic protein peptide and its antagonist Structural implications for the MHC II (I-Au)–peptide complex from docking calculations ppt

... various systems comprised 3899 and 4516 SPC molecules for the agonist and the antagonist in water, respectively, and 817 and 927 Me2SO molecules for the agonist and the antagonist, respectively, corresponding ... the binding and afﬁnity for I-Au because of occupation of the P1 pocket Recent thermodynamic and kinetic studies of the binding of TCRs to peptide MHC ligands suggested that the low afﬁnity of ... (1999) Design and synthesis of a potent cyclic analogue of the myelin basic protein epitope MBP72-85: importance of the Ala81 carboxyl group and of a cyclic conformation for induction of experimental...

Báo cáo khoa học: Pulchellin, a highly toxic type 2 ribosome-inactivating protein from Abrus pulchellus Cloning, heterologous expression of A-chain and structural studies ppt

... Tyr158, Glu2 12, Arg215 and Trp246 in ricin) and another ve conserved residues (Asn 72, Arg 124 , Gln160, Glu195 and Asn196 in abrin -a and abrin-c and Asn78, Arg134, Gln1 72, Glu208 and Asn209 in ricin) ... Journal 27 2 (20 05) 120 1 121 0 ê 20 05 FEBS 120 3 Pulchellin A- chain: cloning and structural studies A A L C Silva et al B C Fig (A) A- chain expression analysis in SDS PAGE, 15% Lane 1, molecular mass ... far-UV CD spectra of rPAC, rPBC, rPAB and native pulchellin CD analyses for the rPAC sample showed a protein prole with predominance of a- helical elements [37]: two negative bands at 22 2 and 20 8...

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