... regulating IVD cell behaviour In this study, we have examined the relative influence of serum, glucose, and oxygen supply, singly or combined, on the growth pattern of bovine IVD cells Since the nutrient ... levels of oxygen and in medium supplemented with 20% FCS and 320 mg/dL glucose Cell proliferation and cell viability Monolayers were harvested by trypsinisation and viable cell counts were performed ... Figure in monolayer and alginate culture systems, are summarised in Table The minimal influence of oxygen levels on IVD cell growth and behaviour is omitted from this summary Discussion alginate...