The role of ORF3 protein in the molecular pathogenesis of porcine circovirus 2 infection

... play a role in determining the pathogenicity of the PCV2 infection (Ramamoorthy et al., 20 09) This thesis describes the role of ORF3 in the pathogenesis of the PCV2 virus infection at the systemic ... the role of ORF3 in the pathogenesis of the virus infection in SPF piglets 28 2. 2 Materials and methods 2. 2.1 Viruses and cell culture The PCV2 were grown in PK-15 C1 cell line of porcine origin ... growth kinetics of PCV2 108 Figure 26 Role of ORF3 in the release of PCV2 from infected cells……… …111 Figure 27 Role of ORF3 induced apoptosis on the In Vivo spread of PCV… 113 Figure 28 Role of Macrophages...

Báo cáo y học: " Molecular characterization of porcine circovirus 2 isolated from diseased pigs co-infected with porcine reproductive and respiratory syndrome virus" pot

... as: Yi and Liu: Molecular characterization of porcine circovirus isolated from diseased pigs co-infected with porcine reproductive and respiratory syndrome virus Virology Journal 20 10 7 :28 6 ... inoculation of conventional pigs with porcine reproductive and respiratory syndrome virus and porcine circovirus J Virol 20 02, 76: 323 2- 323 9 13 Tian K, Yu Y, Zhao T, et al: Emergence of fatal PRRSV variants: ... 13I 91 22 T 13S 92 134 23 V 9N 12I 36T 151 28 T 17P 169 10R 33S 2G 190 19T 14S 12A 191 5R 13A 27 G 20 6 30I 15K 21 0 13E 14D 21 5 8I 37V 29 34T 11A 40 12 R 10S 88 41Q 4E 100 25 F 20 L 101 9Y 9N 1 02 ORF3...

Báo cáo khoa học: "Genetic characterization of porcine circovirus - 2 field isolates from PMWS pigs" potx

... K Identification of Genetic characterization of porcine circovirus- 2 field isolates from PMWS pigs 39 27 28 29 30 31 32 33 34 35 36 porcine circoviruses with genetic variation from lymph nodes ... ORF2 of PCV -2 corresponding to PCV-1s showed a homology of about 63 %, but no detectable cross-reactivity could be shown between ORF2 proteins of PCV-1 and PCV -2 [27 ] ORF2 of Korean PCV -2 isolates ... 1696∼1717 4 12 433 Genetic characterization of porcine circovirus- 2 field isolates from PMWS pigs 33 showed PCV-1 positive in PCR with 886 bp DNA product Two PCV -2 isolates, PC201DJ and PC201SS, showed...

Báo cáo y học: "Prevalence of porcine circovirus-like agent P1 in Jiangsu, China" docx

... Jieyuan Jiang,Aff1 Email: jieyuanj@hotmail.com Aff1 Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences·Key Laboratory of Veterinary Biological Engineering and Technology, ... Technology, Ministry of Agriculture·National Center for Engineering Research of Veterinary Bio-products, Nanjing210014, China Aff2 College of Veterinary Medicine, China Agricultural University, Beijing ... B, Kennedy S, McNeilly F, Allan G: Viral wasting syndrome of swine: experimental reproduction of postweaning multisystemic wasting syndrome in gnotobiotic swine by coinfection with porcine circovirus...

Báo cáo y học: "Development and validation of an ELISA using a protein encoded by ORF2 antigenic domain of porcine circovirus type 2" ppsx

... with a median value of 31.74% These data showed that the assay was repeatable and yielded a low and acceptable variation Evaluation of assay specificity and sensitivity The PCV2 GST -ORF2- E ELISA ... Supernatant of cell lysate after sonication and centrifugation; Lane 4, Pellet of cell lysate after sonication and centrifugation, There was a clear band of 29 kDa (arrow) after induction The protein ... monoclonal antibody and porcine serum Evaluation of GST -ORF2- E proteins ELISA Figure (A) The map of dominant immunoreactive areas of ORF2 The amino acid residues of each area are identified (B) The ORF2...

Báo cáo y học: " Self-assembly of virus-like particles of porcine circovirus type 2 capsid protein expressed from Escherichia coli" pptx

... Virol 20 05, 20 : 129 99-13006 doi:10.1186/1743- 422 X-7-166 Cite this article as: Yin et al.: Self-assembly of virus-like particles of porcine circovirus type capsid protein expressed from Escherichia ... authors declare that they have no competing interests Page of 10 11 12 13 14 15 16 17 18 19 20 21 22 23 Received: 22 May 20 10 Accepted: 21 July 20 10 Published: 21 July 20 10 24 References Tischer ... major capsid protein J Gen Virol 20 00, 81 :22 81 -22 87 Fan H, Ju C, Tong T, Huang H, Lv J, Chen H: Immunogenicity of empty capsids of porcine circovirus type produced in insect cells Vet Res Commun 20 07,...

Báo cáo thú y: " Dynamics of serum antibodies to and load of porcine circovirus type 2 (PCV2) in pigs in three finishing herds, affected or not by postweaning multisystemic wasting syndrome" pdf

... doi:10.1186/1751-0147- 52- 22 Cite this article as: Brunborg et al.: Dynamics of serum antibodies to and load of porcine circovirus type (PCV2) in pigs in three finishing herds, affected or not by postweaning multisystemic ... Comparison of porcine circovirus type load in serum quantified by a real time PCR in postweaning multisystemic wasting syndrome and porcine dermatitis and nephropathy syndrome naturally affected pigs ... Cordioli P, Albina E, Jestin A: Protection of swine against post-weaning multisystemic wasting syndrome (PMWS) by porcine circovirus type (PCV2) proteins Vaccine 20 03, 21 :4565-4575 Brunborg et al...

Báo cáo y học: " Rapid detection of porcine circovirus type 2 using a TaqMan-based real-time PCR" doc

... article as: Zhao et al.: Rapid detection of porcine circovirus type using a TaqMan-based real-time PCR Virology Journal 20 10 7:374 Submit your next manuscript to BioMed Central and take full advantage ... 4 .2 105 24 .49 0 .25 89 1.05 24 .03 0.5597 2. 3 103 30.98 0 .20 92 0.59 30 .22 0.56 32 1.9 SD, standard deviation; CV, Coefficient of variation using a TaqMan real-time PCR that detected a fragment of 26 9 ... Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, 29 01 Beidi Road, Shanghai, 20 1106, People’s Republic of China 3Key Laboratory of Agricultural Genetics and Breeding, Shanghai...

Cell based screening assay for inhibitors of porcine circovirus type2 (PCV2) replication

... to optimize these cell based screening assays for inhibitors of PCV2 replication. Cell Based Screening Assay for Inhibitors of Porcine Circovirus Type 2 (PCV2) Replication ... second messenger assays, (2) reporter gene assays, and (3) cell proliferation assays. Second messenger Cell Based Screening Assay for Inhibitors of Porcine Circovirus Type 2 (PCV2) Replication ... Cell Based Screening Assay for Inhibitors of Porcine Circovirus Type 2 (PCV2) Replication Page | 35 3. RESULTS Results Cell Based Screening Assay for Inhibitors of Porcine Circovirus Type 2 (PCV2) Replication ...

Cell based screening assay for inhibitors of porcine circovirus type2 (PCV2) replication 2

... required tweaking three parameters: 1) cell seeding density, 2) MOI, and 3) duration of infection prior to fixation. Cell Based Screening Assay for Inhibitors of Porcine Circovirus Type 2 (PCV2) Replication Page | 62 4. DISCUSSION For uniformity of results, it was necessary to have an even monolayer of cells per well and any ... rates. U0 126 is a MEK 1 /2 inhibitor found to inhibit PCV2 replication at EC50 of less than 10 µM (Wei and Cell Based Screening Assay for Inhibitors of Porcine Circovirus Type 2 (PCV2) Replication ... infection rates, but instead resulted to greater cell death. The number of cells attached to the bottom of the plate 24 hours post‐seeding was significantly less than the number of attached cells in plates seeded Cell Based Screening Assay for Inhibitors of Porcine Circovirus Type 2 (PCV2) Replication ...

Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf

... Bhagwat SV, Biswas G, Anandatheerthavarada HK, Addya S, Pandak W & Avadhani NG (199 9) Dual targeting property of the N-terminal signal sequence of P450 1A1 Targeting of heterologous proteins to ... open N-terminal signal domain is critical for protein targeting to the ER (Fig 2D) In contrast to the targeting of CYP 1A1 requiring N-terminal truncation, we have shown that intact CYP2B1 and CYP2E1 ... that xenobiotic-inducible CYPs such as rat CYP 1A1 , CYP2E1 and CYP2B1, and mouse CYP 1A1 , contain chimeric noncanonical -targeting signals that are capable of targeting proteins to both the ER and...

Tài liệu Báo cáo khoa học: The role of the ESSS protein in the assembly of a functional and stable mammalian mitochondrial complex I (NADH-ubiquinone oxidoreductase) pptx

... number of differences in the N-terminal domain (located on the matrix side) It is likely that the N-terminal domain is involved in protein protein interactions with other hydrophilic domains of neighboring ... chain was established with succinate and glycerol3-phosphate as substrates Complex II activity was determined after addition of succinate, followed by inhibition by malonate, and complex III activity ... Inspection of the amino acid sequences of the known mammalian ESSS proteins reveals a high degree of conservation in the C-terminal domain (including the transmembrane region), but a signiﬁcant...

Tài liệu Báo cáo khoa học: The role of the SEA (sea urchin sperm protein, enterokinase and agrin) module in cleavage of membrane-tethered mucins pdf

... further investigate the role of the SEA domain in cleavage of membrane-tethered mucins we carried out site-directed mutagenesis of the FRPG ⁄ SVVV site and evaluated hybrid mucins in which the SEA ... previously The fragments were then cloned into the SpeI ⁄ XhoI sites of the polylinker in MUC1FDTR The remaining portions of the polylinker introduced leucine and valine residues N-terminal to the MUC3 ... SEA modules and mucin cleavage T Palmai-Pallag et al Table Conservation of the G ⁄ S cleavage site sequence in MUC1 from several species, other membrane-tethered mucins and SEA domain containing...

Tài liệu Báo cáo khoa học: The molecular surface of proteolytic enzymes has an important role in stability of the enzymatic activity in extraordinary environments pptx

... mutations at these positions in Sendai should promote instability of the surface region As the C region of Sendai also would play an important role in restraining the conformational change of the surface ... temperature indicates the stability of the structure of the protein The difference of the increments between the inactive temperature and the Tm should indicate that the mutations improved the stability ... original activity after h and h of incubation, respectively The stability of Y269A/A271T/Q272R- and Y269A-ALP I in alkaline Ó FEBS 2002 The molecular surface in enzymatic stability in extraordinary environments...

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