Light sheet based fluorescence correlation and cross correlation spectroscopy for quantitative measurements of bio molecules in live cells

... LIGHT SHEET BASED FLUORESCENCE CORRELATION AND CROSS -CORRELATION SPECTROSCOPY FOR QUANTITATIVE MEASUREMENTS OF BIO- MOLECULES IN LIVE CELLS ANAND PRATAP SINGH (M.Sc Chemistry, BHU, INDIA) ... enzyme-kinetics and ligand-receptor stoichiometry [121, 122] and in vivo protein-protein interaction in live cells and even in live organisms [33, 123127] 2.5 Principles and Theoretical Aspects of ... microscopy) and camera based FCS and FCCS (uorescence cross -correlation spectroscopy) , which creates spatio-temporal diffusion and concentration maps of bio- molecules in vitro and in live cells Imaging...

Báo cáo y học: "PKC and PKA Phosphorylation Affect the Subcellular Localization of Claudin-1 in Melanoma Cells"

... junction barrier integrity (7) Conversely, activation of atypical PKC isoforms using bryostatin instead increased the expression of claudin-1 in the tight junction complex, and increased tight ... cell motility We have previously shown that increasing the levels of CLDN-1 increases the invasion of melanoma cells (4) To determine if the increase in claudin-1 needs to be cytoplasmic and not ... nuclear localization of claudin-1 was related to the increased invasive capacity of melanoma cells, we created a claudin-1 expression vector expressing claudin-1 containing a nuclear localization...

Tài liệu Báo cáo khoa học: Analyzing changes of chromatin-bound replication proteins occurring in response to and after release from a hypoxic block of replicon initiation in T24 cells pptx

... The alkaline sedimentation profiles of HeLa and CCRF cells after hypoxia and reoxygenation already revealed [4] that hypoxic incubation caused significant accumulation of initiation competent replicons, ... gassing first mitotic cells appear after about 13 h, their number increases within the next h and decreases again at longer incubation A similar increase of DNA synthesis occurs in the same cells ... contrast, in hypoxic chromatin only very little PCNA was detected However PCNA increased strongly as soon as after reoxygenation and continued to increase after 30 and h The pattern of chromatin-bound...

Báo cáo y học: " Comparison of brachial and carotid artery ultrasound for assessing extent of subclinical atherosclerosis in HIV: a prospective cohort study" pot

... (DEA) software (Montreal, Quebec) Average diameter of brachial artery (before and after dilation) was obtained from nine sequential images taken at rest and 12 taken during peak artery dilation ... L, Shaw AG, Busfield F, Haluska B, Barnett A, Kesting J, Short L, Marczak M and Shaw JT: Carotid artery intimal medial thickness, brachial artery flow-mediated vasodilation and cardiovascular risk ... Guidelines for the ultrasound assessment of endothelial-dependent flow-mediated vasodilation of the brachial artery: A report of the international brachial artery reactivity task force J Am Coll Cardiol...

The study of interactions of transmembrane receptors and intracellular signaling proteins in live cells by fluorescence correlation and cross correlation spectroscopy

... THE STUDY OF INTERACTIONS OF TRANSMEMBRANE RECEPTORS AND INTRACELLULAR SIGNALING PROTEINS IN LIVE CELLS BY FLUORESCENCE CORRELATION AND CROSS -CORRELATION SPECTROSCOPY LIU PING (M.Sc), CAS A THESIS ... C-terminus domain, to expose binding sites for Src-homology-2 (SH2) domain-containing proteins and phosphotyrosine-binding (PTB) domain-containing proteins Adaptor proteins with a SH2-binding domain ... ligands bind to the extracellular domains of two receptor subunits and form a 2:2 complex (42), resulting in activation of the intracellular kinases of the receptors Upon ligand binding, the...

Báo cáo y học: "Direct Vpr-Vpr Interaction in Cells monitored by two Photon Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Imaging" pps

... Figure Direct Vpr-Vpr interaction in HeLa cells visualized by FLIM Direct Vpr-Vpr interaction in HeLa cells visualized by FLIM Cells were transfected with the DNA construct encoding eGFP or eGFP-Vpr ... Sensitivity of CFP/YFP and GFP/mCherry pairs to donor photobleaching on FRET determination by fluorescence lifetime imaging microscopy in living cells Microsc Res Tech 2006, 69:933-939 Merzlyak EM, ... Clamme JP, Azoulay J, Mely Y: Monitoring of the formation and dissociation of polyethylenimine/DNA complexes by two photon fluorescence correlation spectroscopy Biophys J 2003, 84:1960-1968 Publish...

Application of bayesian model selection in fluorescence correlation spectroscopy (FCS) to WNT3EGFP secretion and diffusion in zebrafish embryos

... extended to the model determination of EGFP labeled proteins in living zebrafish embryos FCS has then been employed to investigate in vivo EGFP tagged Wnt3 (Wnt3EGFP) secretion and diffusion ... proteins remains problematic In this study, a Bayesian model selection approach has been applied to evaluate FCS data of fluorescent proteins in vitro and in vivo to address the issues of competing ... fitting to evaluated models 56 Figure 3.2 Bayesian analysis of Fluorescein with varying excitation intensity 57 Figure 3.3 Bayesian analysis of mixtures of Atto565 and Atto565-streptavidin...

Characterization of diffusion behavior of a novel extra cellular sphingolipid associated peptide probe by fluorescence correlation spectroscopy and imaging total internal reflection fluorescence correlation spectroscopy

... Characterization of Diffusion Behavior of a Novel Extra- cellular Sphingolipid Associated Peptide Probe by Fluorescence Correlation Spectroscopy and Imaging Total Internal Reflection Fluorescence ... cell membrane organization traced by SBD, two new biophysical tool Imaging Total Internal Reflection -Fluorescence Correlation Spectroscopy (ITIR-FCS) and Imaging Total Internal Reflection -Fluorescence ... Thorsten Wohland, and Rachel Kraut Alternate raft pathways cooperate to mediate slow diffusion and efficient uptake of a sphingolipid tracer to degradative vs recycling pathways Journal of Cell Science...

Quantification of biomolecule dynamics and interactions in living zebrafish embryos by fluorescence correlation spectroscopy

... fiber cells. We finally analyze the FCS data with the anomalous subdiffusion model and study the molecular crowdedness of cells in living embryos. Chapter 5 describes the direct quantification of protein‐protein interactions in living zebrafish embryos with ... use in zebrafish embryos and demonstrate several applications of FCS in living zebrafish embryos, showing that single molecule‐based studies in living organism are possible: The autofluorescence ... foreign genes in zebrafish embryos are discussed and compared with that in cell cultures. Secondly, the autofluorescence in living zebrafish embryos, in particular the autofluorescence ...

Investigation of microfluidics in channels and tissues by fluorescence correlation spectroscopy (FCS)

... dynamics including diffusion, local concentration, and binding rate (67; 68) Scanning FCS Since traditional point FCS is more suitable for fast-diffusing molecules, in case of slow-diffusing or ... Fluorescence correlation microscopy (FCM), a technique term ﬁrst introduced in 1995 by Terry and coworkers (93) which combines imaging techniques with FCS, identiﬁed points of interest within a sample and ... determine protein dynamics and molecular interactions The use of two SPCMs in combination with a 50:50 non-polarizing beamsplitter allows the cross correlation measurement of SPCM signals eliminating...

Báo cáo hóa học: "Robust System and Cross-Layer Design for H.264/AVC-Based Wireless Video Applications" pptx

... Proposed video and transport features for different applications with performance in terms of delay and average PSNR for different RLC-PDU loss rates and QCIF video sequence Foreman coded at 7.5 fps Video ... account multiple users in a wireless system are not further discussed For some specific video- related issues and system design of schedulers and network buffers we refer, for example, to [47] 5.3 ... throughput ηAL for different system designs for RLC-PDU loss rate of 1% (left-hand side) and 10% (right-hand side) For both cases, we assume that the considered user is also the worst-case user for which...

Investigation of peptide lipid interaction by fluorescence correlation spectroscopy

... and cell membrane organization investigated by imaging fluorescence cross‐ correlation spectroscopy. " Biophys J 97(9): 2630‐9. I Investigation of Peptide Lipid Interaction by Fluorescence Correlation Spectroscopy ... provides further guidelines for the rational design of antimicrobial peptides. VIII Investigation of Peptide Lipid Interaction by Fluorescence Correlation Spectroscopy List of Figures Fig 1.1 Schematic representation of the Gram‐negative bacteria cell wall. ... Phase of clinical trial Investigation of Peptide Lipid Interaction by Fluorescence Correlation Spectroscopy Table 1.1 Clinical developments of cationic antimicrobial peptides 14 Investigation of Peptide Lipid Interaction by Fluorescence Correlation Spectroscopy ...

Development of a fluorescence correlation spectroscopy method for the study of biomolecular interactions

... products labeled with the same fluorescent dye, the only way of differentiating the product from the reactant is when the product has a molecular mass that differs from the reactants by at least a factor ... of the dual-color complexes formed This easily distinguishes the products from the free reactants via the amplitude of the CCF, as compared to the weak dependence of the ACF with the mass of the ... (2.40) For fittings of cross -correlation amplitudes (in chapters 3—5), the software package Mathematica 5.0 (Wolfram Research Inc., Champaign, IL) was used to model the changes of CCF amplitudes...

A correlation based method for direction finding of multipath signals in frequency hopping systems

... communication systems In smart antenna systems, the direction of users is an important factor to increase the capacity, and an antenna array is usually used at the base station to estimate and track ... hopping signals, and a new method is proposed to track multipath frequency hopping signals In Chapter 2, several popular methods for DOA estimation are addressed including maximum likelihood methods, ... using DOA, TOA and TDOA The direction parameters are usually estimated with an antenna array which has a certain geometric shape, and the estimation task is performed by exploiting the data collected...

INVESTIGATION OF MEMBRANE PROPERTIES IN THE CENTRAL NERVOUS SYSTEM OF DROSOPHILA MELANOGASTER STUDIED BY FLUORESCENCE CORRELATION SPECTROSCOPY

... Summary The objective of this study is to apply biophysical fluorescence technique, i.e fluorescence correlation spectroscopy (FCS), in situ in the central nervous system of fruit fly (Drosophila melanogaster) ... neuronal membrane fluidity was being studied in situ in the central nervous system of Drosophila melanogaster embryos by FCS Our approach promises to shed light on the biophysical features of cellular ... this is the first time confocal FCS is being used to study membrane fluidity in situ in the central nervous system of Drosophila melanogaster embryos A study by Sergent et al indicated that increased...

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