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ACCURATE ALIGNMENT OF SEQUENCING READS FROM VARIOUS GENOMIC ORIGINS

ACCURATE ALIGNMENT OF SEQUENCING READS FROM VARIOUS GENOMIC ORIGINS

ACCURATE ALIGNMENT OF SEQUENCING READS FROM VARIOUS GENOMIC ORIGINS

... genome The challenges of alignment of SGS reads are composed of different error profiles of sequenced reads from different sequencing technologies, short read lengths (reads from SGS can be ~36 ... high volume of reads from various wet-lab/drylab origins accurately and efficiently Hence, a salvo of alignment methods was designed to handle these reads 3.1 The aim of all genomic alignment algorithms ... ACCURATE ALIGNMENT OF SEQUENCING READS FROM VARIOUS GENOMIC ORIGINS LIM JING QUAN (B.CompSc.(Hons), NUS) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY IN COMPUTER...
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Báo cáo y học: "Antimicrobial Susceptibilities of Brucella Isolates from Various Clinical Speciemens"

... hospital of the Medical Faculty of Van Yuzuncu Yil University (Van, Turkey) Identification methods: Identification of species was made on the basis of the requirement of CO2 for growth, production of ... susceptibility patterns of Brucella spp appear to vary geographically Therefore, we suggest, regional periodic assessment of susceptibility of strains to antimicrobials The results of this in vitro study ... Aksaray S, Yetener V, Akinci E, Colpan A, Erbay A Biotypes and antimicrobial susceptibilities of Brucella isolates Scand J Infect Dis 2003; 35(5):337-338 Kửse S, Kiliỗ S, Ozbel Y Identification of...
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Báo cáo y học:

Báo cáo y học: " Simultaneous alignment of short reads against multiple genomes" pps

... formation of branches, which constitute the parallel blocks that account for the nonlinearity (a) Short reads Alignments (b) Short reads Alignments (c) Short reads Alignments Figure alignments against ... methods Compared with the "reference only" alignments, the graph alignments increased the number of recovered SNPs by 15%, of deletions by 22.6%, and of insertions by 37.2% (Table 1) In particular, ... against multiple genomes Efficient Efficient alignments against multiple genomes (a) Only reads that are sufficiently similar can be aligned against a single reference (b) Separate alignment against...
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Báo cáo y học: " Enrichment of sequencing targets from the huma" pptx

... out the less-likely possibility of a systematic error in the sequencing In 18 of the discrepancies, the replicate sample was unable to make a high-quality call The majority of these positions (72%) ... comparison of their coverage distribution, we normalized the coverage by dividing the observed coverage of each base by the mean coverage of all the targeted bases For all four samples, the peak of the ... 48% 0.1% Before enrichment After enrichment Figure Efficiency of target enrichment Efficiency of target enrichment The pie chart on the left illustrates the relative percentages of the targeted...
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Báo cáo khoa học: The fabp4 gene of zebrafish (Danio rerio) ) genomic homology with the mammalian FABP4 and divergence from the zebrafish fabp3 in developmental expression pot

Báo cáo khoa học: The fabp4 gene of zebrafish (Danio rerio) ) genomic homology with the mammalian FABP4 and divergence from the zebrafish fabp3 in developmental expression pot

... 17 and 24 hpf (A) Distribution of fabp3 transcripts (A1, A 2) in the diencephalon (Di), hindbrain (Hb), spinal cord (Sc), and somites (So), and fabp4 transcripts (A3, A 4) in the retina (Re) of ... in the specificity and affinity of ligand binding by mammalian FABP4 [23] 1624 The phylogenetic relationship of zebrafish fabp4 and the H6-FABP gene from Antarctic fishes was determined using amino ... 4) The phylogenetic analysis resolved fish FABP4 (and H6-FABPs) and FABP3 (and H8-FABPs) as proteins encoded by distinct genes, and suggested that these genes are orthologs of mammalian FABP4 and...
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Báo cáo khoa học: Purification, characterization, cDNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, Psacothea hilaris ppt

Báo cáo khoa học: Purification, characterization, cDNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, Psacothea hilaris ppt

... PCR and sequencing A QuickPrepÒ Micro mRNA Purification Kit (Amersham Bioscience) was used for isolation of mRNA from P hilaris larval midguts First-strand cDNA synthesis from the isolated mRNA and ... degradation activity of P hilaris cellulase against crystalline cellulose, Avicel (Merck) was used under the same conditions as the CMCase assay Optimal pH for P hilaris cellulase activity against ... N-Terminal amino-acid sequencing analysis indicated that the mature protein of P hilaris cellulase was a truncated form, which lacked a signal peptide composed of the first 21 amino acids Therefore, the...
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Báo cáo khoa học:

Báo cáo khoa học: " Early growth performance of salal (Gaultheria shallon) from various North American west-coast locations" pot

... (1) with zijk relative growth increment of the plant volume from 1992 to 1993 of the k-th plant of the i-th seedlot in the j-th block; ( 92 v ijk ) volume of the k-th plant of the i-th seedlot in ... For explanation of the plot see legend to figure 2a Growth performance of Gaultheria shallon 603 Table II Summary of the p-values of all the performed ANOVAs Variables Source of variability Seedlots ... by the results of the analyses of variance (table II) However, only the volumes of plants grown from seedlots and 23 were significantly different from more than one of those grown from the other...
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Báo cáo khoa học:

Báo cáo khoa học: "Differences in vegetation cover resulting from various methods of site preparation for pine plantations in South Africa" potx

... shown in table IV for each of the surveys The vegetation cover was best controlled in disked plots Disking combined with total weed control reduced the vegetation to 2.9% in cover, 0.1 % in height, ... the seedling grade on vegetation regeneration and growth is minimal within the st year after planting Soil cultivation treatments included pitting, augering, ripping (subsoiling), and ripping and ... article examines changes in species composition, height, area cover and biomass of competing vegetation in response to forest regeneration methods applied after harvesting the first rotation of trees...
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Báo cáo y học:

Báo cáo y học: "Improved variant discovery through local re-alignment of short-read next-generation sequencing data using SRMA" pot

... Page 12 of 12 doi:10.1186/gb-2010-11-10-r99 Cite this article as: Homer and Nelson: Improved variant discovery through local re-alignment of short-read next-generation sequencing data using SRMA ... Local re-alignment of simulated data Local re-alignment of empirical data To assess the performance of local re-alignment on a dataset with a known diploid sequence, two whole genome human re -sequencing ... respectively, and after SRMA were 0.434, 0.538, and 0.328, respectively This demonstrates the ability of SRMA to improve variant calling, especially for indels To further examine the accuracy of the variant...
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báo cáo khoa học:

báo cáo khoa học: " Microsatellite analysis of Damask rose (Rosa damascena Mill.) accessions from various regions in Iran reveals multiple genotypes" ppsx

... same microsatellite profile This group included all accessions from the main rose oil production sites of Damask rose in Iran The pattern of this group was identical to that of an accession from ... that the origin and centre of diversity of Damask roses can be found in this region In Iran, cultivation and consumption of Damask roses has a long history Crude distillation of roses was probably ... dominant scores of microsatellite alleles, among all accessions of Figure rose included in this genetic Damask 1a UPGMA clustering of Dice genetic similarities based on dominant scores of microsatellite...
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Báo cáo y học:

Báo cáo y học: "Discovery of biological networks from diverse functional genomic data" ppt

... networks from diverse genomic data lies in the heterogeneity and high noise levels in large-scale data sets High-throughput data by nature are often noisy and simple combinations of results from ... network discovery Previous work in identifying groups of genes involved in specific biological pathways from interaction networks has focused on mainly binary http://genomebiology.com/2005/6/13/R114 ... whereas only 25 were identified by either physical or genetic evidence, and only 18 by microarray evidence alone Different evidence types have varying degrees of relevance for different pathways -...
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Fast and accurate mapping of next generation sequencing data

Fast and accurate mapping of next generation sequencing data

... due to sequencing errors For NGS sequencers like Illumina and SOLiD, the majority of sequencing errors are of this type The first contribution of this thesis is the introduction of a fast and memory-efficient ... overview of the importance and applications of genomic sequencing We will now present a review of the technologies behind genome sequencing 2.4.1 Sanger Sequencing Sanger sequencing uses the idea of ... from an algorithmic point of view, processing the output of sequencing machines pose two distinct challenges; the volume of the data and sequencing errors The volume of the data will keep on increasing...
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Accurate and sensitive determination of selected contaminants from food packaging materials

Accurate and sensitive determination of selected contaminants from food packaging materials

... implementation of food safety policies This thesis will cover two broad areas of contaminants from food contact materials: Determination of the migration of bisphenolic monomers from canned coatings into food, ... migration of various types of toxic contaminants from food packaging materials into oily, aqueous and acidic food matrices The first part of the project focuses largely on the development and optimization ... Migration of monomers / additives from polymers used in food contact materials 1.6 State -of- the-art analytical methods for determining amount of 18 contaminants from food packaging materials...
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Removal of congo red from wastewater by adsorption onto waste red mud

Removal of congo red from wastewater by adsorption onto waste red mud

... Namasivayam, and C and Technol., by by solutions K of waste congo slurry red J 53 : 153 - 157 (1992) N Removal waste : 32 - 42 N Removal celluosic Removal biogas by Technol Muniasamy, (1996) T ... Prot R wastewater and by hydroxide Yamuna, R adsorption Waste T on Dye waste Management, : 14 (1994) S., Prasad, G and chrome dye from aqueous : ash fly by : Indian - Jeyakumar, removal red Adsorption ... removal The be from effectively of congo adsorption isotherms byproduct, a bauxite used as adsorbent for red from wastewaters followed The an processing both Langmuir Langmuir adsorption and...
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Báo cáo y học:

Báo cáo y học: "ISOLATION OF CHLAMYDIA PNEUMONIAE FROM SERUM SAMPLES OF THE PATIENTS WITH ACUTE CORONARY SYNDROME"

... endothelium (20) In the present paper we report, that viable elementary bodies of C pneumoniae with typical electron microscopic structure can be isolated from the serum samples of the patients with ... patients with acute coronary syndrome Furthermore, using combination of bacteriological and PCR-based methods we show herein that patients with acute coronary syndrome have higher C pneumoniae detection ... obtained from the initial group, serum specimens obtained from the patients with ACS had a positive TaqMan PCR assay with variations in bacterial load from 200 to 2000 copies/ml of serum However serum...
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