THE MECHANISM OF PPARN3 MEDIATED DOWN REGULATION OF SODIUM HYDROGEN EXCHANGER 1 (NHE1) GENE EPXRESSION AND ITS INHIBITION BY ESTROGEN RECEPTOR n1 5
THE MECHANISM OF PPARN3 MEDIATED DOWN REGULATION OF SODIUM HYDROGEN EXCHANGER 1 (NHE1) GENE EPXRESSION AND ITS INHIBITION BY ESTROGEN RECEPTOR n1 5
... PPARγ -mediated NHE1 down- regulation From the results shown above, we concluded further that the DNA binding ability of ERα is involved in inhibiting PPARγ -mediated NHE1 repression 15 1 15 2 Figure ... 30% reduction in NHE1 mRNA in ER positive MDA-MB-2 31 cells It is interesting that in the absence of fulvestrant, 1 M of 15 d-PGJ2 brought down NHE1 mRNA to 50 % of the control 14 2 However, after ... alone Together, these data confirm that re-expression of ERα in MDA-MB-2 31 and its activation by estrogen present in serum blocks PPARγ -mediated down- regulation of NHE1 expression 14 3 Figure...
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THE MECHANISM OF PPARN3 MEDIATED DOWN REGULATION OF SODIUM HYDROGEN EXCHANGER 1 (NHE1) GENE EPXRESSION AND ITS INHIBITION BY ESTROGEN RECEPTOR n1 1
... 1. 1.5 Ligands and physiological functions of PPARα and PPARδ 1. 1.6 Ligands of PPARγ 1. 1.7 PPARγ and adipogenesis 10 1. 1.8 PPARγ and insulin sensitization 12 1. 1.9 ... PPARs and cancer 13 1. 1 .10 PPARγ and breast cancer 15 1. 2 ESTROGEN RECEPTORS (ERS) 18 1. 2 .1 Identification and structures of ERs 18 1. 2.2 Mechanism of action of ... PPARγ -MEDIATED REGULATION OF NHE1 68 3A .1 PPARγ AND THE EXPRESSION OF NHE1 68 3A1 .1 Identification of putative PPRE on NHE1 promoter 68 3A1.2 Down- regulation of NHE1 by PPARγ ligands...
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THE MECHANISM OF PPARN3 MEDIATED DOWN REGULATION OF SODIUM HYDROGEN EXCHANGER 1 (NHE1) GENE EPXRESSION AND ITS INHIBITION BY ESTROGEN RECEPTOR n1 2
... ROS/RNS 10 5 contributes approximately 20 % of the overall down- regulation of NHE1 transcription by 5M of 15 d-PGJ2 10 6 Figure 11 : ROS/RNS contributes to down- regulation of NHE1 (A) MCF-7 (3 X105 cells/6-well ... (Figure 11 C) In contrast, 15 d-PGJ2 caused 61% reduction in NHE1 mRNA in the absence of NAC We reasoned that this difference in magnitude of down- regulation in NHE1 expression by 15 d-PGJ2 in 10 3 the ... confirm the role of PPARγ receptor in PPARγ ligand- 88 mediated down- regulation of NHE1 gene expression Besides, the presence of the putative PPRE on NHE1 promoter and PPARγ ligands’ ability to down- regulate...
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THE MECHANISM OF PPARN3 MEDIATED DOWN REGULATION OF SODIUM HYDROGEN EXCHANGER 1 (NHE1) GENE EPXRESSION AND ITS INHIBITION BY ESTROGEN RECEPTOR n1 4
... EFFECT OF PPARγ ON NHE1 Given that the presence of estrogen blocked the down- regulation of NHE1 expression induced by PPARγ lgiands, we next investigated the role of estrogen receptor in PPARγ -mediated ... protein and mRNA is mediated by estrogen recptor alpha 13 1 Figure 19 : Re-expression of ERα blocks PPARγ -mediated down- regulation of NHE1 expression in MDA-MB-2 31 cells (A) MDA-MB-2 31 (2X105 cells/6-well ... abrogates the inhibitory effect of E2 on PPARγ -mediated down- regulation of NHE1 Since re-expression of ERα and its activation by E2 in ER negative MDA-MB2 31 cells rescued the inhibition of NHE1 expression...
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THE MECHANISM OF PPARN3 MEDIATED DOWN REGULATION OF SODIUM HYDROGEN EXCHANGER 1 (NHE1) GENE EPXRESSION AND ITS INHIBITION BY ESTROGEN RECEPTOR n1 6
... basis and biological significance." Oncogene 25( 51) : 67 31- 67 48 Bunone, G., P A Briand, et al (19 96) "Activation of the unliganded estrogen receptor by EGF involves the MAP kinase pathway and direct ... hepatotoxicity: another lesson from troglitazone." Diabetologia 51( 11) : 210 8 211 6 Jurka, J and A Milosavljevic (19 91) "Reconstruction and analysis of human Alu genes." J Mol Evol 32(2): 10 5 -12 1 Kallinowski, ... Barouki (19 98) "Down- regulation of cytochrome P450 1A1 gene promoter by oxidative stress Critical contribution of nuclear factor 1. " J Biol Chem 273( 41) : 269 69- 269 76 Morrison, R F and S R Farmer (19 99)...
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THE MECHANISM OF PPARN3 MEDIATED DOWN REGULATION OF SODIUM HYDROGEN EXCHANGER 1 (NHE1) GENE EPXRESSION AND ITS INHIBITION BY ESTROGEN RECEPTOR n1 3
... to 15 d-PGJ2, while 15 d-PGJ2 resulted in the maximal reduction in the number of colonies formed by MDA-MB-2 31 cells expressing the highest level of PPARγ receptor 11 2 Figure 14 : PPARγ agonists ... cells and only a 36 % down- regulation in T47D cells (Figure 14 D) Interesetingly, the extent of inhibition on cologenic ability of different breast cancer cells mirror-imaged the level of PPARγ receptor ... reseeded at a density of 1. 0 X104 cells /10 0mm dish After 14 days of incubation, cells were stained with crystal violet (C) MDA-MB-2 31 (2 X 10 5 cells/6-well dishes) and T47D (3 X105 cells/6-well dishes)...
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Tài liệu Báo cáo khoa học: Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase docx
... mgÆmL)1 BSA and stored at )20 °C Electrogenic BSP uptake inhibition by antibodies The kinetics of bilitranslocase transport activity inhibition by antibodies were examined by preincubating 24 lL ... addition of protein (2.6 ± 0.04 lmolÆmin)1Æmg protein)1, with lg valinomycin), as well as of K+ (6.25 ± 0.16 unitsÆmEq)1 K+, with lg valinomycin) and valinomycin (0.51 ± 0.03 unitsÆlg)1 valinomycin, ... also investigated in carnation petal microsomes by preincubating them with antibody A in the presence of increasing concentrations of bilirubin BSP uptake was assayed to track the progress of the...
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Human Physiology: The Mechanism of Body Function - part 1 pdf
... Endocrine/Explorations/Endocrine Function Chapter 11 1 1- 1 Muscular/Anatomy/Skeletal Muscle 1 1- 3 Muscular/Histology/Cardiac Muscle Muscular/Histology/Smooth Muscle xxvi 1 1- 4 1 1- 5 1 1- 6 1 1- 8 1 1- 1 2 1 1- 1 5 1 1- 1 8 1 1- 1 9 1 1- 2 0 1 1- 3 1 ... Tape Tape Chapter 11 1 1- 8 Tape 1 1- 1 2 Tape 1 1- 1 6 Tape Chapter 15 1 5-6 Tape 1 5-8 Tape 1 5-2 5 Tape Chapter 16 1 6-6 Tape 1 6 -1 4 Tape Chapter 6 -1 6 -1 2 6 -1 8 6 -1 9 1 0-2 Tape 1 0-7 Tape 1 1 Module Module Module ... Chapter 12 1 2-2 Nervous/Exploration/Motor and Sensory Pathways 1 2-8 Nervous/Explorations/Reflex Arc Chapter 13 1 3 -1 5 Nervous/Anatomy/Gross Anatomy Chapter 14 1 4 -1 1 4-7 1 4-8 1 4 -1 2 1 4 -1 4 1 4 -1 5 1 4 -1 6 1 4-2 0...
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Báo cáo y học: " Mechanism of HIV-1 Tat RNA translation and its activation by the Tat protein" ppsx
... Tat1 and Tat2 RNAs in the untreated RRL system Translation of Tat1 and Tat2 RNAs in the untreated RRL system The top three lanes depict the Glob-Fluc, UTRTat1-Rluc and UTRTat2-Rluc RNAs used in the ... one of the DNA fragments corresponding to the complete 5' UTR of Tat1 and Tat2 RNAs, to the deleted 5' UTR (delta R-U5) of Tat1 and Tat2 RNAs, to the 5' UTR of the viral genomic RNA, and to the ... http://www.retrovirology.com/content/6/1/74 Figure Translation of the Tat RNA in the RRL system Translation of the Tat RNA in the RRL system A Structure of the recombinant Glob- and EMCV RNA templates...
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Báo cáo khoa học: Binding of the viral immunogenic octapeptide VSV8 to native glucose-regulated protein Grp94 (gp96) and its inhibition by the physiological ligands ATP and Ca2+ Ming Ying and Torgeir Flatmark pot
... RGYVYQGL (VSV8) and physiological low-molecular mass ligands of the ER lumenal compartment (Ca2+ and ATP) , known to regulate the function of Grp94 as a protein chaperone [4,11,12] Thus, the SPR binding ... ligands of the chaperone in the ER lumenal compartment which may perturb this interaction Binding of the viral immunogenic octapeptide VSV8 to native rGrp94 Native glycosylated rat Grp94 (rGrp94) and ... low-affinity binding sites for VSV8 Binding of ATP, MgATP and NECA to rGrp94 The function of Grp94 as a protein chaperone is reported to be sensitive to ER lumenal ATP levels [11] The chaperone...
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Regulation of na+ h+ exchanger 1 (NHE1) stability in PTEN mouse embryonic fibroblasts
... exchanger- 1 (NHE1) 1. 1 .1 NHE1 isoforms and NHE1 structure 1. 1.2 Regulation of NHE1 1. 1.3 Physiological function of NHE1 1. 1.4 Role of NHE1 in cancer progression 11 ... formation of Hsp90/Akt/NHE1 complex in MCF10AT1 12 4 3.3.3 Akt regulates NHE1 protein expression in MCF10CA1a 13 1 3.3.4 NHE1 interacts with Akt in MCF10CA1a and not MCF10CA1h 13 4 3.3.5 ... 14 4 CHAPTER 4: DISCUSSION 15 1 4 .1 NHE1 is a potential client protein of Hsp90 in MEFPTEN-/- cells 15 1 4 .1. 1 Increased stability of NHE1 protein in MEFPTEN-/- cells in comparison...
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Redox regulation of estrogen receptor alpha and sodium hydrogen exchanger 1 gene expression by hydrogen peroxide
... REGULATION OF NHE1…………… 11 0 3B .1 DOWN -REGULATION OF NHE1 BY H2O2 11 0 3B .1. 1 Identification of crucial region in the promoter of NHE1 11 0 3B .1. 2 H2O2 down-regulates 0 .15 kb proximal NHE1 promoter ... signaling 21 1.3.4 Regulation of estrogen receptors 23 1. 3.5 Estrogen and estrogen receptors in human breast cancer 27 v 1. 4 THE SODIUM HYDROGEN EXCHANGER 29 1. 4 .1 pH regulation ... turnover 16 1. 3 ESTROGEN RECEPTOR 17 1. 3 .1 Estrogen and estrogen receptors: Introduction 17 1. 3.2 Estrogen receptor and genomic signaling 20 1. 3.3 Membrane and cytoplasmic...
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Báo cáo khoa học: Characterization of testis-specific serine–threonine kinase 3 and its activation by phosphoinositide-dependent kinase-1-dependent signalling doc
... phosphorylation of GST–TSSK3 with [32 P]ATP[cP] The phosphorylation reactions of GST–TSSK3K39R by Myc-PDK1 [32 ] immunoprecipitated from 293T cells and of HA–TSSK3K39R immunoprecipitated from 293T cells, by ... maximum activation and ⁄ or activation by PDK1 of TSSK3, as suggested for PKCf phosphorylation and activation by PDK1 [35 ] Thus we hypothesize that in order to efficiently recruit PDK1 to TSSK3, cofactors ... the regulation of TSSK3 activity and suggest a similar mechanism of activation to that of the AGC kinase family For a number of AGC kinases the 3- phosphoinositide-dependent protein kinase- 1 (PDK1)...
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Báo cáo Y học: The mechanism of nitrogen monoxide (NO)-mediated iron mobilization from cells NO intercepts iron before incorporation into ferritin and indirectly mobilizes iron from ferritin in a glutathione-dependent manner pot
... isonicotinoyl hydrazone class as effective antiproliferative agents II The mechanism of action of ligands derived from salicylaldehyde benzoyl hydrazone and 2-hydroxy-1-naphthylaldehyde benzoyl hydrazone ... controls Apolactoferrin was from Calbiochem A polyclonal rabbit anti-(human ferritin) Ig was from Roche Diagnostics All other chemicals were of analytical reagent quality The NO- generators and other ... identifying the exact molecular nature of the Fe released by NO and the transporter involved are underway Finally, out current results may be important in understanding the cytotoxic actions of NO...
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Báo cáo khoa học: The interaction of the Escherichia coli protein SlyD with nickel ions illuminates the mechanism of regulation of its peptidyl-prolyl isomerase activity ppt
... between the nickel ion and the protein is the key feature of the binding process Further evidence that SlyD interacts with nickel ions with a : stoichiometry is provided by the analysis of the changes ... Structure and interactions of SlyD L Martino et al The constant of binding of the reaction Kb is dened as: Kb ẳ ẵPL ẵP ẵL 3ị The total concentrations of the protein [P]tot and the nickel ions [L]tot ... Structure and interactions of SlyD A B Fig Structure mapping of the chemical shift perturbations for the PPIase domain of SlyD upon nickel ion binding The positions of residues that disappear in the 15...
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Từ khóa: Báo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018Một số giải pháp nâng cao chất lượng streaming thích ứng video trên nền giao thức HTTPNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinChuong 2 nhận dạng rui rochuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)BÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namMÔN TRUYỀN THÔNG MARKETING TÍCH HỢPQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ