... oxaloacetate was 3.6-fold greater t hanthat of malate and 21-fold greater t han that of the sum of citrate and isocitrate. I n addition,14CO2-labeling indicatedthat oxaloacetate was one of ... carbon dioxide-fixing enzyme in the reductive tricarboxylic acid cycle Tadayoshi Kanao, Mineko Kawamura, Toshiaki Fukui, Haruyuki Atomi and Tadayuki ImanakaDepartment of Synthetic Chemistry and ... purification of the recombinant enzyme In order to c onstruct an expression vector for Cl -idh,twooligonucleotides (sense, 5Â -A AAAACATATGGCAAGCAAATCGACCATCATCTACAC-3Â, and antisense, 5Â-AAAAAGGATCCCGGCTGAAAACCGGGCTGCATTA-3Â)were...