... primer),SIV2-D 5’ GGCACTATTGGAGCTAAGAC 3’ (reverseprimer), SIV-P 6FAM-AGATTTGGATTAGCA-GAAAGCCTGTTGGA-TAMRA (TaqMan probe). Thesignal was finally compared to a standard curve of known concentrations from ... BIOQUAL, Inc. Rockville, MD, according tostandards and guidelines as set forth in the Animal Wel-fare Act and The Guide for the Care and Use of Laboratory Animals, as well as according to animal ... subcutaneously with PMPA, 20 mg/kg/day, and FTC, 50 mg/kg/day.Quantitative assay for SIVmac251 viral RNA levelsFor measurement of plasma SIVmac251 RNA levels, a quantitative TaqMan RNA reverse...