...
(Hickey
et
al,
1990).
Original
article
Molecular
cloning
and
partial
sequence
characterization
of
the
duplicated
amylase
genes
from
Drosophila
erecta
L
Bally-Cuif,
V
Payant,
S ...
between
the
2
copies
of
the
gene
within
a
locus.
We
cloned
both
copies
of
the
amylase
gene
from
D
erecta
and
sequenced
part
of
the
coding
region...
... corres-
ponding to the first 18 residues of the N terminus and
the last 16 residues of the C terminus of the predicted
mature protein. No peptide corresponding to the predic-
ted transit peptide sequence ... of
Synechococcus and Prochlorococcus, and the other
consisting of Anabaena–Nostoc, Synechocystis, and
Thermosynechococcus. These two clades correspond to...
... of the
53 kDa spot (5.3) was identical to a pI value calcu-
lated from the amino acid sequence of GmPDIL-3a,
and the isoelectric point of the 57 kDa spot (5.1) was
consistent with that from the ... organelle and membrane proteins are synthe-
sized and folded with the assistance of molecular chap-
erones and other folding factors in the endoplasmic
reticul...
... b-conglycinin during
the course of the folding process of these proteins.
Results
Cloning and expression of GmPDIL-1 and
GmPDIL-2
To clone the soybean ortholog of Arabidopsis
PDI-like 1-1 and PDI-like ... eighth
and ninth introns of GmPDIL-1 had the consensus
sequence CTNAN [34]. In the case of GmPDIL-2,
10 of the 11 introns had TTNAN, and the eighth...
... of
CYP719A3, the amount of nandinine produced was estima-
ted by using the calibration curve of (S)-scoulerine (pmol
versus peak area) and the absorption ratio of (S)-scoulerine
and (S)-nandinine ... samples and open circles are untreated controls. The relative expression level shows the
values standardized by that of control (0 h sample) as 1. The error bar indica...
... [15],
suggesting the existence of additional OSCs. As part of an
effort to understand the molecular mechanism of the
triterpene cyclization reactions, as well as the molecular
evolution of these proteins ... with
Kpn I and XbaI, and ligated to the sites of pYES2
(Invitrogen), to obtain pYES2-LcIMS1 in which the ORF
of the cDNA was ligated to the GAL1 promot...
... the
N-terminal sequence of the human b
1
. However, the
C-terminal sequence was significantly different from that
of the human b
1
, suggesting that it might encode a novel
splice variant of the ... lm(C).
Ó FEBS 2003 Cloning and characterization of Na
+
channel b
1B
(Eur. J. Biochem. 270) 4767
Fig. 4. The effect of b
1B
and b
1
subunits on the function an...
... suggest that the complete silencing of
the gene transcript could not be achieved due to dilu-
tion of dsRNA in the feeding stage of the female tick
[31]. On the other hand, Acosta and coworkers
obtained ... concentration
of dsRNA, the region of homology between the
dsRNA and the target, as well as other lesser know
mechanisms [29]. In recent investigations it was...
... work, we
describe the cloning of the gene sequence of BmKIM, the
functional expression of the recombinant toxin in Escheri-
chia coli and its effect on the sodium channels of neurons
and ventricular ... as
primers, and the BmKIM genomic DNA as template for
PCR, the nucleotide sequence obtained was the same as the
cDNA. Therefore, there is only one intron...
... cycle of dynein, bind-
ing of ATP to the primary ATPase site of AAA1
causes dissociation of dynein from the microtubule,
and binding of the MTBD to the microtubule acceler-
ates the dissociation of ... around the hinges (A). Binding of ATP to the trailing head
(red) releases the MTBD from the microtubule and then (B) the head is pulled forward by the...