Báo cáo sinh học: "Electroporation by nucleofector is the best nonviral transfection technique in human endothelial and smooth muscle cells" pdf
Báo cáo sinh học: "Electroporation by nucleofector is the best nonviral transfection technique in human endothelial and smooth muscle cells" pdf
... AccessMethodologyElectroporation by nucleofector is the best nonviral transfection technique in human endothelial and smooth muscle cellsNina Iversen*, Baard Birkenes, Kari Torsdalen and Srdjan DjurovicAddress: ... participated in the design and coordination of the study, established the transfection methods electropora-tion, nucleofection and PCI, and supervised the followingoptimalization of these methods. ... (Figure 2).After the voltage settings had been established the optimalresistance and capacitance were found. For CoA and AoSMC the best resistance setting was found to be in the area725–900Ω...
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Báo cáo y học: " Real time analysis of b2-adrenoceptor-mediated signaling kinetics in Human Primary Airway Smooth Muscle Cells reveals both ligand and dose dependent differences" ppt
... esponse by the probe (i.e. an increase in the emission ratio from base-line) and (2) the rate of this response. Analyses involvedcomparing responses to isoproterenol and a range ofclinically ... statesresulting in the favouring of one downstream pathwayover another which has intriguing therapeuticimplications.Despite the recent expansion of studies investigatingligand-directed signaling in ... recom-binant b2-adrenoceptor activation in HEK293 and CHOcells following exposure to both endogenous (epinephr-ine and norepinephrine) and synthetic (isoproterenol,fenoterol and terbutaline)...
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Báo cáo sinh học: "Estimation by simulation of the efficiency of the French marker-assisted selection program in dairy cattle (Open Access publication)" ppsx
... variance explained by this QTL. Thisweighted mean information content is presented in Table III for candidates ofyears 2004 and 2006 and for their sires and dams. For all the traits, informationcontent ... Tab. I).Therefore, the MAS evaluation is more demanding in computational terms but the information on QTL is increasing with time. More families are genotyped and QTL transmission is better ... shown, the gain of efficiency achieved by improving the accuracyof the pid is important but to obtain even larger gains, other MAS strategiesmust be applied (such as the use of linkage disequilibrium).Some...
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Báo cáo khoa học: Active c-secretase is localized to detergent-resistant membranes in human brain pptx
... active siteof the protease [8]. Assembly of the complex is initiated in the ER, where Aph-1 and nicastrin interact, followed by binding of PS. Thereafter, Pen-2 binds to the com-plex and facilitates ... better in SH-SY5Y cellsthan in brain samples. Difficulties in obtaining pureDRM fractions from brain tissue are probably due to the heterogeneity of the sample and high levels ofmyelin. In the ... APP and APP-CTFswere distributed to fractions 4 and 5. The procedurewas repeated using rat brain, and the results were in line with those obtained for human brain (Fig. 2B,D).However, in rat...
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báo cáo khoa học: "Overexpression of LCMR1 is significantly associated with clinical stage in human NSCLC" ppt
... important clinico-pathological role in the lung cancer. It will be of consid-erable interest to further understand these interactions and elucidate the intrinsic mechanism s, since one of the most ... analysis of LCMR1 in normal human tissues. ZL prepared GST-LCMR1 protein and antibody. CL participated in the qPCR and drafted the manuscript. ZL and XM performed immunohistochemistry analysis. ... CL and YL carried out qPCR. YZ, ZY, and PW collected the cases and sections. LCparticipated in the design and coordination and supervised the whole study.All authors read and approved the final...
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Báo cáo toán học: "Tight Upper Bounds for the Domination Numbers of Graphs with Given Order and Minimum Degree, II." pdf
... δ) is a non-increasing function in δ. We are interested in determining the numbers δk(n)whereδk(n)=max{δ |γ(n, δ) ≥ k},k≥ 1.Since the domination number of an (n, δ)-graph G is 1 if and ... +1≥√2n −1.Since this is larger that the previous bound, we obtain the desired lower bound for |T |. The following theorem is immediate from Lemmas 3 and 5.Theorem 8 If T ⊂ Zn, n ≥ 6 is a symmetric, ... Actually there were six undecided entries in the table given in [2], three of which are decided in Section 5 of this paper. The remainingthree unknown entries are marked by asterisks. The values...
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Báo cáo sinh học: " Liver mitochondrial dysfunction is reverted by insulin-like growth factor II (IGF-II) in aging rats" doc
... in the pathogenesis of disease and aging [7,8].Normal mitochondrial function is a critical place in maintaining cellular homeostasis because mitochondriaproduce ATP and they are the major intracellular ... is char-acterised by a decrease in lean body mass and anincrease in adipose mass, osteopenia, muscle atrophy,reduced exercise tolerance and changes in the plasmalipoprotein profile [2]. These ... gradient (result-ing in an increase of MMP) and ATP synthesis and reduced free radical generatio n by mitochondria, protonleak rate and the vulnerability for pore opening in ANTwhich was associated...
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báo cáo sinh học:" How labour intensive is a doctor-based delivery model for antiretroviral treatment (ART)? Evidence from an observational study in Siem Reap, Cambodia" potx
... operator and six PLWHAs – two involved in counselling, two in keeping files and guiding patients to the doctors, and two in organizing home visits of irregular and defaulter patients. In August ... reduction in length of consultations. The most impor-tant gains were in the pre-ART and in ART initiationphases, with lesser gains in the ART follow-up phases. In the Siem Reap clinic, these reductions ... assumptions for a district-wide ART scale-up may notbe realistic, but the extrapolation is mainly intended toreveal the stakes in the domain of human resources. Thereare many districts in sub-Saharan...
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Báo cáo sinh học: "Protein kinase CK2a is overexpressed in colorectal cancer and modulates cell proliferation and invasion via regulating EMT-related genes" docx
... JZ and HLperformed the statistical analysis and drafted the manuscript. DW and LLhelped in drafting the manuscript and contributed specific information and critical analysis throughout the ... 100%), according to the percen-tages of the posit ive staining areas relative to the entirecarcinoma-involved area or, for the normal samples, the entire section. The sum of the intensity and extentscores ... on the basis of his-tological and clinical findings at the Nanfang Hospitalbetween 2005 and 2007. Prior patient consent and approval from the Institute Research Ethics Committeewere obtained...
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Báo cáo sinh học: "Bisphosphonate-associated osteonecrosis of the jaw is linked to suppressed TGFb1-signaling and increased Galectin-3 expression: A histological study on biopsies" pot
... interpreted the data and harvested the samples. KAestablished the immunohistochemistry, analyzed the tissue samples,interpreted the data, and performed the histopatholgic analysis of BRONJ-related and ... mucoperiostealtissue in BRONJ tissues might be explained by the increased Galectin-3, which is known to mediate inhi-bition of intraoral inflammation [1,16]. Galectin-3 wasshown to specifically inhibit ... cytoplasmic immunohistochemicalstaining and the spatial pattern of expression, the label-ing index was determined as the number of positivelystained cells per total cells in the visual field. Multiplemeasurements...
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