... RNA in bovine esopharyngeal fluid as determined by real-time RT-PCR. Report of the Session of the Research Group of the Standing Tech-nical Committee of the European Commission for the Control ... RNA.Amplification was performed in a 25 μL reaction mixusing the following program: 42°C of cDNA synthesis for 1 hour, 95°C for 15 min, 30 cycles of 1 min at 95°C, 1min at 55°C, and 1 min at 72°C and ... competing inter-ests.Authors' contributions The corresponding author, Sazaly AbuBakar is the princi-pal investigator of the study, was involved in the design,supervision, data analyses and...