Báo cáo y học: " Gene expression profiling of human prostate cancer stem cells reveals a pro-inflammatory phenotype and the importance of extracellular matrix interactions" pps
Báo cáo y học: " Gene expression profiling of human prostate cancer stem cells reveals a pro-inflammatory phenotype and the importance of extracellular matrix interactions" pps
... weredominated by the signaling of inflammatory cytokinesthrough the JAK-STAT (Janus activated kinase-signal trans-ducer and activator of transcription) pathway and the interac-tion of cell surface ... same system, as the focal adhesion pathway is activated by extracellular matrix- receptor interaction. Changes in extracellular matrix and associated proteins have been reported in the metastaticprogression ... significantlydifferent in prostate cancer stem cells. Functional annotation of this signature identified the JAK-STAT pathway and focal adhesion signaling as key processes in the biology of cancer stem...
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Báo cáo y học: "Gene expression profiling in murine autoimmune arthritis during the initiation and progression of joint inflammation" pps
... results obtained with RNA from normal paws of naive BALB/cSCID mice (AN), clinically normal pre-arthritic paws (PA), acutely arthritic paws (AA) and chronically inflamed paws (CA).Available online ... progression and effi-cacy of treatment, or to predicting the prognosis of the dis-ease. The major obstacles in the human studies are the relatively late diagnosis of RA, the large variety of cell types (cells ... experimental processes and in the finalization of the manuscript. EGB controlled Affymetrix hybridization and scanning experiments, managed preliminary data analysis and finalized the manuscript....
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Báo cáo y học: "Gene expression profiling in the synovium identifies a predictive signature of absence of response to adalimumab therapy in rheumatoid arthritis" doc
... 5'-ggtcatggagatgtc-cgtaa-3' and 3'-accaatagagagaccaggaagaa-5'; GTSE1: 5'-acgtgaacatggatgacccta-3' and 3'-gttcgggaaccggattattta-3';CDC2: 5'-ggtcaagtggtagccatgaaa-3' ... 5'-ggtcaagtggtagccatgaaa-3' and 3'-ccaggagggata-gaatccaag-5'; and MKI67: 5'-ccccaaccaaaagaaagtctc-3' and 3'-gactaggagctggagggctta-5'.Histopathology and immunohistochemistry on paraffin-embedded ... 5'-ttcttggaggatgcagctaaa-3' and 3'-aagcccaaccaacaaagagtt-5'; IL-6: 5'-gcccagctatgaactccttct-3' and 3'-tgaagaggtgagtggctgtct-5'; INDO: 5'-ggtcatggagatgtc-cgtaa-3'...
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Báo cáo y học: "Gene expression profiling in circulating endothelial cells from systemic sclerosis patients shows an altered control of apoptosis and angiogenesis that is modified by iloprost infusion" potx
... endothelial cells RNA extraction. She also provided the statistical analysis. MD and PAperformed the gene array analysis. RA helped in the flow-cytometric analysis of endothelial cells. LC and PA were responsible ... K,Imai H, Harada M, Osanai H, Kondo T, Murohara T: The impact of the capability of circulating progenitor cell to differentiate on myocardialsalvage in patients with primary acute myocardial ... usingTaqMan SDS analysis software. For each amount of RNA tested triplicate Ct values were a veraged. BecauseCt values vary linearly with the logarithm of the amount of RNA, this average represents...
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Báo cáo y học: " Gene expression profiling of Hfe-/- liver and duodenum in mouse strains with differing susceptibilities to iron loading: identification of transcriptional regulatory targets of Hfe and potential hemochromatosis modifiers" pdf
... chain reaction; SAM, statistical analysis of microarrays; TCA, tricarboxylic acid.Table 8Sequences of the primers used for validation of microarray results by real-time PCR Gene GeneBankaccessionForward ... AF_5034445'-AAG CAG GGC AGA CAT TGC GAT-3' 5'-CAG GAT GTG GCT CTA GGC TAT GT-3' 1.98Hamp2 AY_2328415'-AAG CAG GGC AGA CAT TGC GAT-3' 5'-GGA TGT GGC TCT AGG CTC TCT ATT-3' ... conceptual expertise for functionalannotation, and MM for statistical analysis. All authors read and approved the final version of the manuscript.Additional data files The following additional data...
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Báo cáo y học: "Gene expression profiling reveals novel TGFβ targets in adult lung fibroblasts" docx
... for-ward AAC AAG AAC TAC GCA ATG GGG and reverse GTCCTC TGT TCA TAG CCT TCT G; nuclear receptor co-repressor2 (NCOR2): forward CAG CAG CGC ATC AAG TTC AT and reverse GTA ATA GAG GAC GCA CTC AGC; ... fibrosis. A combination of the Affymetrix change algorithm (Microarray Suite5) and of analysis of variance models was used to identify TGFβ-regulated genes. Additional criteriawere an average up- ... and participated in the microarray work, performed immunohistochemistrystaining, and drafted the manuscript. DJA participated in the design and coordination of the study and in the prep-aration...
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Báo cáo y học: "Degradome expression profiling in human articular cartilage" potx
... articular cartilage and has beenshown to play a role in collagen cleavage in at least a subset of OA patients [5].Cathepsin O and cathepsin Z are also highly expressed genesin cartilage and, again, ... Osteoarthritis Cartilage 2009, 17:547-555.45. Nakayama Y, Nara N, Kawakita Y, Takeshima Y, Arakawa M, KatohM, Morita S, Iwatsuki K, Tanaka K, Okamoto S, Kitamura T, Seki N,Matsuda R, Matsuo ... compared with normal, with CFI, C2 and DF increas-ing and CFB decreasing. In the complement pathway, C2 ispart of the classical pathway and CFB and DF are part of the alternative pathway with...
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báo cáo hóa học:" Gene expression profiling for molecular distinction and characterization of laser captured primary lung cancers" ppt
... instruc-tions of the manufacturer, using commercially availableassays-on-demand (Applied Biosystems, Applera Deut-schland GmbH, Darmstadt, Germany). Ct values were cal-culated by the ABI PRISM software, ... statistical analysis The quality control, normalization and data analysis, wereassured with the affy package of functions of statisticalscripting language 'R' integrated into the Bioconductorproject ... isolation, cRNA labeling and hybridization to microarrays The described procedures strictly adhered to the guide-lines from the Microarray Gene Expression Data Society and the MicroArray Quality...
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Báo cáo y học: "Gene expression induced by interleukin-17 in fibroblast-like synoviocytes of patients with rheumatoid arthritis: upregulation of hyaluronan-binding protein TSG-6" doc
... transcription system. The recombinant RNAwas quantified by the measurement of A 260 and used as a standard (after cDNA synthesis) in the quantitativeRT–PCR reaction.Quantitative PCR analysisFor quantification, ... Toivanen P: Expression of the myc-family proto-oncogenes and relatedgenes max and mad in synovial tissue. Scand J Rheumatol1999, 28:314-318.37. Hashiramoto A, Sano H, Maekawa T, Kawahito Y, ... CCA GGC TTC CCA AAT GAG TAGRO-β 2 GGC CAT TTT CTT GGA TTC CT3 CCG AAG TCA TAG CCA CAC TCIL-6 2 GAA GAG CCC TCA GGC TGG ACT G3 AGT AAC TCC TTC TCC ACA AGC GCIL-8 2 TCT CAG CCC TCT TCA AAA ACT...
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Báo cáo y học: "Gene expression profile and synovial microcirculation at early stages of collagen-induced arthritis" pot
... the overlying softtissues, the patella tendon was transversally cut and the prox-imal and distal part carefully mobilized. After exposure, the 'Hoffa's fatty body' was superfused ... Feldmann M, Brennan FM, Williams RO, Woody JN, Maini RN: The transfer of a laboratory based hypothesis to a clinically usefultherapy: the development of anti-TNF therapy of rheumatoidarthritis. ... resistant FVB animals markedly. The inflam-matory cell response, as indicated by the enhanced activation and interaction of leucocytes with the microvascular endothe-lium, was mirrored by the expression...
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