... Phusion polymerase (Finnzyme, Finland). PCR cyclecondition was: 1 cycle at 98°C for 30 sec, followed by 30cycles at 98°C f or 10 sec, 61°C for 30 sec, 72°C for 15sec and a final cycle at 72°C ... spsim@fmhs.unimas.myFaculty of Medicine and Health Sciences, Universiti Malaysia Sarawak, Lot 77,Seksyen 22 KTLD, Jalan Tun Ahmad Zaidi Adruce, 93150 Kuching, Sarawak,MalaysiaYee and Sim Journal ... at higher density(lanes 2, 3, 5 and 6) as compared to those seeded a tlower density (lanes 1 and 4). Subsequently, Southernhybridization was perf ormed using a DNA probe hybri-dizing to the...