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Báo cáo y học: "Gene prediction: compare and CONTRAST" pptx

Báo cáo y học:

Báo cáo y học: "Gene prediction: compare and CONTRAST" pptx

... prediction over time. The genesensitivity and specificity and the exon sensitivity and specificity on theEGASP test set [5] are shown for several programs by year of initialpublication. Included ... sequences.By comparison, the accuracy of the Ensembl evidence-based genepredictions used in the EGASP experiment at the gene level were 71.6%sensitivity and 67.3% specificity and 77.5% sensitivity and ... splice sites and nearly universal start and stop codon sequences. These signals are highly conserved,are relatively easy to model, and have been the focus of anumber of algorithms trying to locate...
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Báo cáo y học:

Báo cáo y học: "Gene expression profile and synovial microcirculation at early stages of collagen-induced arthritis" pot

... wasrecently shown to bind the P-selectin glycoprotein ligand 1 –a molecule that plays a role in homing of T lymphocytes [32].The role played by the cytokine receptor IL-13 receptor α1 inarthritis ... postcapillary synovial venulesLeucocytes rolling along the endothelium of postcapillary synovial venules. Shown are the proportions of leucocytes rolling along the endothelium of postcapillary synovial ... mono-cytes, B cells and activated synoviocytes is triggered. This cel-lular infiltration, together with production of a complex array ofcytokines and other soluble mediators, contributes to synovialproliferation,...
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Báo cáo Y học: Cloning, chromosomal localization and characterization of the murine mucin gene orthologous to human MUC4 pdf

Báo cáo Y học: Cloning, chromosomal localization and characterization of the murine mucin gene orthologous to human MUC4 pdf

... extractedfrom parotid, submaxillary gland, salivary glands, trachea,stomach, liver and gallbladder, duodenum and intestine and kidney by using guanidine hydrochloride as previouslydescribed [13].RT-PCR ... map,subclone and sequence; (c) 5¢ and 3¢ ends are usually verysimilar between genes from the same class; and (d) clonesmay show instability presumably due to the repetitivestructure of sequences and ... duodenum and intestine (lane 7) and kidney (lane 8) was used. (A) One band of324 bp is obtained with the couple of primers NAU764 and NAU726.(B) The efficiency of the cDNA synthesis was estimated by...
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Báo cáo y học:

Báo cáo y học: "Gene expression profiling in murine autoimmune arthritis during the initiation and progression of joint inflammation" pps

... were collected, and live cells (lymphocytes) wereseparated on Lympholyte-M (Cedarlane, Ontario, Canada).Finally, 2 × 107 lymphocytes were injected intraperitoneallyon days 0 and 7 into recipient ... Proteoglycan-inducedpolyarthritis and spondylitis adoptively transferred to naive(nonimmunized) BALB/c mice. Arthritis Rheum 1990,33:866-876.31. Mikecz K, Glant TT: Migration and homing of lymphocytes tolymphoid and synovial ... expression by providing genetic and clinical homogeneity, and an opportunity to monitor theonset and progression of the disease [12-20]. DNA micro-array technology was successfully applied to...
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Báo cáo y học: "Characterization of histopathology and gene-expression profiles of synovitis in early rheumatoid arthritis using targeted biopsy specime" pot

... study, we analyzed gene-expression profiles in the syn-ovial lining tissues in situ in early RA using synovial specimensobtained by targeted biopsy, followed by LCM and cDNAmicroarray analyses. ... in early RA. That is, we prepared synovialspecimens from early RA by targeted biopsy under arthros-copy, and analyzed gene-expression profiles in the synovial lin-ing tissues selected by LCM ... changes in the synovium of patientswith early RA may improve our understanding of this diseaseentity and aid diagnosis in the future.Biopsy targeting of articular lesions in synovial tissues...
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Báo cáo y học: "Retinoid X receptor and peroxisome proliferator-activated receptor-gamma agonists cooperate to inhibit matrix metalloproteinase gene expression" potx

... 4Protein levels and collagenolytic activity more strongly inhibited by dual treatment with LG268 and rosiglitazoneProtein levels and collagenolytic activity more strongly inhibited by dual treatment ... a polyclonal MMP-13 antibody generously provided byPeter Mitchell (Pfizer Inc, New York, NY, USA). Protein bandswere visualized by incubation with a goat anti-rabbit secondaryantibody conjugated ... analysis and contributed equally to thiswork. YR was responsible for acquisition of data, analysis and interpretation of data, and manuscript preparation. MBS wasresponsible for study design and...
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Báo cáo y học:

Báo cáo y học: "Gene expression and activity of cartilage degrading glycosidases in human rheumatoid arthritis and osteoarthritis synovial fibroblast" potx

... cartilage-degradingglycosidases was moderately downregulated byproinflammatory cytokines including TNFα, IL-1β and IL-17.Conclusions According to our present data, glycosidasesexpressed by synovial membranes and ... MMPs,proinflammatory cytokines and chemokines [13].There is increasing evidence that SFs are key players in thepathogenesis of RA by invading and eroding hyaline cartilage.SFs, activated locally, produce ... stillpoorly understood. Enzymes that collaborate to determine thefinal structures of glycans are glycosyl transferases and gly-cosidases. The significance of glycosidases has been recentlysuggested...
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Báo cáo y học:

Báo cáo y học: "BioGPS: an extensible and customizable portal for querying and organizing gene annotation resources" doc

... symbol is not the most com-monly used symbol in the literature (for example, TP53 and P53). Finally, new online resources are continually beingdeveloped, and staying abreast of these tools and ... assays)https://products.appliedbiosystems.com/ab/en/US/adirect/ab?cmd=search&type=GEKEY&filter=GENE_SYMBOL&limit_to=ALL&species=H_SAPIENS,M_MUSCULUS,R_norvegicus&keyword={{Symbol}}Cell Signaling Techologyhttp://www.cellsignal.com/search/keyword.action?keyword={{Symbol}}GeneTex antibodieshttp://www.genetex.com/commerce/catalog/srhkeyword.cz?keyword= {{Symbol}}Invitrogen ... plugin library additionallyallows users to rank plugins by utility, as measured by theentire community's usage of each plugin in BioGPS layouts.Although BioGPS superficially is a simple...
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Báo cáo y học:

Báo cáo y học: " High cell density and latent membrane protein 1 expression induce cleavage of the mixed lineage leukemia gene at 11q23 in nasopharyngeal carcinoma cell line" ppt

... Phusion polymerase (Finnzyme, Finland). PCR cyclecondition was: 1 cycle at 98°C for 30 sec, followed by 30cycles at 98°C f or 10 sec, 61°C for 30 sec, 72°C for 15sec and a final cycle at 72°C ... spsim@fmhs.unimas.myFaculty of Medicine and Health Sciences, Universiti Malaysia Sarawak, Lot 77,Seksyen 22 KTLD, Jalan Tun Ahmad Zaidi Adruce, 93150 Kuching, Sarawak,MalaysiaYee and Sim Journal ... at higher density(lanes 2, 3, 5 and 6) as compared to those seeded a tlower density (lanes 1 and 4). Subsequently, Southernhybridization was perf ormed using a DNA probe hybri-dizing to the...
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Báo cáo y học:

Báo cáo y học: "Gene polymorphisms in APOE, NOS3, and LIPC genes may be risk factors for cardiac adverse events after primary CABG" pdf

... chromosome7q36.Hepatic lipase (LIPC) is a lipolytic enzyme synthesized inhepatocytes playing a major role in HDL metabolism. Ittakes part in hydrolysis of triacylglycerides and phosphol-ipids of HDL2 into ... events.Arterial Hypertension was evident at the time of primarysurgery in 73% of our patients and did significantlyTable 1: Demographics of patients and medical therapy at the time of primary CABGPatients ... sec-ondary CAD prevention after primary coronary arterybypass grafting (CABG). Appearance of cardiac adverseevents after primary CABG is frequent and leads to recur-rent angina, myocardial...
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