... grouping of objects. They have two distinct types of
nodes, and edges only connect a node of one type to a node of the other. For example, the proteins of a biological system
could be the nodes of ... are the yeast two-
hybrid (Y2 H) system [23] and affinity purification followed
by mass spectrometry (AP-MS) [24]. The Y2 H system assays
whether proteins can physically i...
... Boston, USA)
presented the results of a thorough benchmarking of the
yeast two-hybrid system. He convincingly showed that
interactions discovered in high-throughput yeast two-hybrid
screens were ... much about the abstract properties of
biological networks, often with little application to day-to-day
biology, and the achievement of amazing milestones such as
the first draf...
... anticipated. These factors significantly
reduced the power of the study. Based on their findings, the
authors estimate that more than 100 hospitals would be
needed to show the 30% difference they sought. ... goal of identifying
and treating patients early in the course of their illness is
preferable to waiting until more serious signs and symptoms
have developed. Certain...
... although the HGGG loop was a
critical structural element for the catalytic efficiency of the
enzyme, the cold adaptation of the enzyme could not be
attributed to the presence of the Gly cluster ... than
the native cold adapted enzyme (Table 1). The mutant
G261A /Y2 69A exhibits an E
a
almost the same as in the case
ofthenativeenzyme(Table1).
Thermal inactivation of...
... the content of secondary structure only after the
disruption of the native tertiary structure of RNase A
(Figs 2–4). Interestingly, the preservation of the tertiary
structure coincides with the ... hydro-
chloride (GdnHCl), urea or elevated temperatures, they
destroy the tertiary structure of proteins which results in the
loss of enzymatic activity. Regarding the...
... when studying the catalytic
efficiency for these mutants in the diaphorase reaction, all
of them yield values very close to those of the wild-type
enzyme at the different ionic strengths assayed (within ... FNR the side-chain of E139 might influence the
properties of the flavin, assuming some of the functions
carried out by E301 in the wild-type enzyme [22]. In this
c...
... complement of chromosomes.
The fidelity of chromosome transmission is enhanced not
only by the geometry of stable centromere–MT interac-
tions, but also by the action of a centromere-based
regulatory system ... way, the critical connections that hold sisters
together from S phase through early mitosis are literally cut
by Ômolecular scissorsÕ in the form of Esp1/separase at...
... allergies are ‘ the most common
chronic condition in Canadians 12 years of age and
older.’’
1
In one study, 42% of children were diagnosed
with AR by the age of 6 years. The prevalence of AR has
increased ... for the
clinician to be comfortable with the side-effect profile and be able to discuss potential safety concerns regarding these therapies.
Among the safety concern...
...
1
Institute of Psychiatry, University of Bologna, Italy,
2
Department of Psychology, Catholic University, Milan, Italy and
3
Department of
Psychiatry, San Raffaele Scientific Institute, Milan, Italy
Email: ... bipolar
disorder of Mr. A daughter strengthen the genetic hypoth-
esis. On the contrary, only Mrs. B mother aunt was
affected by depression and anxiety, pharmacologica...
... for
ankylosing spondylitis and rheumatoid arthritis, an absence of
fibromyalgia, and a temporal increase in the prevalence of
gout. What should we make of all of these estimates and why
are they important ... regarding
rheumatic disease etiology.
Zeng and colleagues reviewed diverse studies, many of them
using the Community Oriented Program for the Control of
Rheumatic Disea...