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Báo cáo y học: " Model-based analysis of two-color arrays (MA2C)" ppsx

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Báo cáo y học: "Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells"

... to identify key molecules in the course of hy-poxia-induced apoptotic pathways. Such analysis of trophoblastic cells will, in the near future, yield in-sights into the mechanisms of preeclampsia ... biology and biochemistry have enabled analy-sis of the expression profiles of numerous proteins at once. In this study, we looked at hypoxia-induced apoptosis and, by using the Western array ... probably by inhibiting the expression of Bcl-2 and by modulating phosphorylation of JNK. Table 2 Altered protein expression of JAR cell with anti-Bag-1 antisense oligo nucleotide induced by hypoxia...
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Báo cáo y học: " Mutation Analysis of hCDC4 in AML Cells Identifies a New Intronic Polymorphis"

... of hCDC4 can also be observed in Acute Myeloid Leukemia (AML) or high risk Myelodysplastic Syndrome (MDS). Therefore we carried out a mutational analysis of the hCDC4 gene in 35 samples of ... Results Mutational analysis of hCDC4 in AML In this study the common exons 2 to 11 and the three known transcript variants of exon 1 of hCDC4 (Figure 1A) were analyzed by direct DNA sequencing ... Sequence analysis of hCDC4 exon 10 identifies a new intronic SNP During the analysis of exon 10 and adjacent intronic sequences we could for the first time identify a new single nucleotide polymorphism...
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Báo cáo y học: " Transcriptome analysis of murine thymocytes reveals age-associated changes in thymic gene expression"

... levels of many of these genes identified in the array analysis. Moreover, Western blot analysis of thymo-cyte lysates also confirmed that IgM increases with age (Figure 3). Flow cytometry analysis ... and array analysis. For each array sample, the RNA was prepared from the purified thymocytes. The thymocytes were processed by using the RNEasy Mini Kit (Qiagen, Valencia, CA). Quality and ... the thymus (as previously shown by flow cytometry, Figure 4), it appears that the increased production of immunoglobulin detected in DNA microarrays and PCR within the aged thymocytes is...
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Tài liệu Báo cáo Y học: Functional analysis of DM64, an antimyotoxic protein with immunoglobulin-like structure from Didelphis marsupialis serum pdf

Tài liệu Báo cáo Y học: Functional analysis of DM64, an antimyotoxic protein with immunoglobulin-like structure from Didelphis marsupialis serum pdf

... potentiated by enzymatic degradation of phospholipids [7,44].The capacity of DM64 to inhibit the myotoxicity inducedby myotoxins I (Asp49) and II (Lys49) from B. asper venomwas analyzed in vivo ... threedomains and are present in the inhibitory receptors of Fig. 6. Inhibition of in vitro cytotoxicity of myotoxins I or II by DM64.Cytotoxicity was analyzed in vitro using C2C12 skeletal muscle ... aromatic residues phenylalanine and tyrosine typical of the Ig-fold are bold in each domain.Fig. 5. Inhibition of in vivo myotoxicity of myotoxins I or II by DM64.Groups of four mice were injected...
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Tài liệu Báo cáo Y học: Functional analysis of a small heat shock/a-crystallin protein from Artemia franciscana docx

Tài liệu Báo cáo Y học: Functional analysis of a small heat shock/a-crystallin protein from Artemia franciscana docx

... H.S., Han, Y. & Fung, B.K K. (2000)Subunit e xchange of small h eat sho ck proteins. Analysis of olig-omer formation of aA-crystallin and Hsp27 by fluorescence res-onance energy transfer ... chaperoning in vitro [66], and disassembly of active unitsfrom an oligomeric (storage) state of a-crystallin wasproposed, upon structural analysis of aA-crystallin by site-directed spin labelling, ... variants produced byp26-ND60 and p26-alpha were pr esent but recognizedpoorly by antip26 antibody. To determine if recognition of p26-ND60 and p26-alpha encoded polypeptides by antibodyto p26 was...
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Báo cáo Y học: The analysis of the fine specificity of celiac disease antibodies using tissue transglutaminase fragments pot

Báo cáo Y học: The analysis of the fine specificity of celiac disease antibodies using tissue transglutaminase fragments pot

... formation of gamma glutamyl-lysine bonds [4].Furthermore, tTG plays a role in the transduction of extracellular signals, mediated by its additional GTP-hydrolyzing activity [5], analogous to that of ... member of a family of seven isoforms of enzymesinvolved in protein cross-linking, including prostatic TGaseand factor XIII. tTG is a Ca2+-dependent ubiquitousintracellular enzyme that catalyzes ... H2O2and TMB.Fig. 2. Western blots of 12 purified tTG fragments. (A) Primary antibody, mAb His-probe. (B) Primary antibody, mAb CUB 7042. Secondaryantibody, goat anti-(mouse Ig) conjugated with...
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Báo cáo Y học: Structural analysis of Francisella tularensis lipopolysaccharide potx

Báo cáo Y học: Structural analysis of Francisella tularensis lipopolysaccharide potx

... 2-N-(3-O-acyl4-acyl2)-b-GlcN-(1–6)-2-N-acyl1)3-O-acyl3-GlcN whereacyl1,acyl2and acyl3are 3-hydroxyhexadecanoic or 3-hy-droxyoctadecanoic acids, acyl4is tetradecanoic or (minor)hexadecanoic ... Acyl1,acyl2and acyl3residues had hydroxy or acyloxy groups at C-3 (13C signals of C-3 at 69.0–72.4 p.p.m.), while acyl4had no substituents.The signals of acyl chains could only be identified ... N-acylated with acyl2. All acyl C-1 signals wereidentified from H-2:C-1 HMBC correlations. C-1 of acyl2gave HMBC correlation to H-2 of GlcN B; C-1 of acyl4showed correlation to H-3 of acyl2....
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Báo cáo Y học: Structural analysis of deacylated lipopolysaccharide of Escherichia coli strains 2513 (R4 core-type) and F653 (R3 core-type) pot

Báo cáo Y học: Structural analysis of deacylated lipopolysaccharide of Escherichia coli strains 2513 (R4 core-type) and F653 (R3 core-type) pot

... chemical analysis of 7-O-(2-amino-2-deoxy-a-D-glucopyranosyl-L-glycero-D-manno-heptose as a constituent of the lipopolysaccharides of theUDP-galactose epimerase less mutant J-5 of Escherichia ... describedpreviously [13].Preparation of deacylated LPS of E. coliF653LPS (2.11 g) was de-O-acylated by mild hydrazinolysis(yield 1.425 g) and 902.5 mg were further subjected toalkaline de-N-acylation ... of carbon. Additionally,long-range correlations between protons of adjacent sugarresidues across the glycosidic bond established theirsequence; this was confirmed by the analysis of a NOESYspectrum...
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Báo cáo Y học: Kinetic analysis of hydroxylation of saturated fatty acids by recombinant P450foxy produced by an Escherichia coli expression system docx

Báo cáo Y học: Kinetic analysis of hydroxylation of saturated fatty acids by recombinant P450foxy produced by an Escherichia coli expression system docx

... 2002Kinetic analysis of hydroxylation of saturated fatty acidsby recombinant P450foxy produced by anEscherichia coliexpressionsystem7Tatsuya7Kitazume1, Akinori Tanaka1, Naoki Takaya1, ... fundamentally asdescribed previously [15] by gas chromatography (GC) andGC-electron impact-mass spectrometry (GC-EIMS). Priorto analysis, the carboxyl group of the products was blockedby methylation ... University of Tokyo, JapanCytochrome P450foxy (P450foxy, CYP505) is a fused pro-tein of cytochrome P450 (P450) and its reductase isolatedfrom the fungus Fusarium oxysporum, which catalyzes thesubterminal...
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Báo cáo Y học: Conformational analysis of opacity proteins from Neisseria meningitidis ppt

Báo cáo Y học: Conformational analysis of opacity proteins from Neisseria meningitidis ppt

... M., Meyer, T.F.& Gray-Owen, S.D. (2000) Carcinoembryonic antigen familyreceptor specificity of Neisseria meningitidis Opa variantsinfluences adherence to and invasion of proinflammatory cyto-kine-activated ... described by Davies et al. [22]. The expression of wild-type OpaJ129 was determined by Western blottingusing monoclonal antibody 15-1-P5.5 [18]. The expression of wild-type OpaB128 was determined by ... reached anoptical density of 660 nm (D660) of 0.6, isopropyl thio-b-D-galactoside (IPTG) (Boehringer Mannheim, Germany)was added to a final concentration of 1 mM.After3hofincubation at 37...
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