Báo cáo y học: " Cloning and characterization of microRNAs from wheat (Triticum aestivum L )" docx
Báo cáo y học: " Cloning and characterization of microRNAs from wheat (Triticum aestivum L.)" docx
... *Key Laboratory of Crop Heterosis and Utilization (MOE) and State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of ... below spikes, leaves, flag leaves, roots and spikes, were probed with labeled oligonucleotides. The tRNA and 5S RNA bands were visualized by ethidium bromide staining of polyacrylamide gels and ... across all major lineages of plants, including mosses, gymnosperms, monocots and dicots; for example, AthmiR166, miR159 and miR390 areconserved in all lineages of land plants, including bryophytes,lycopods,...
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Báo cáo Y học: Cloning and characterization of novel snake venom proteins that block smooth muscle contraction pptx
... resulting from application of calciseptine, a well-characterized neurotoxin from blackmamba (Dendroaspis polylepis polylepis).Calciseptineisaknown blocker of L- type Ca2+channels, a property thatunderlies ... mMdithiothreitol in the presence of 0.5MTris/HCl,pH 8.5, 6Mguanidine hydrochloride, and 2 mMEDTA ina volume of 0.5 mL. Three microliters of 4-vinylpyridinewere then added, and alkylation was allowed ... include the conotoxins from cone snails, agatoxins from spiders, and scorpion toxins [11–16]. These toxins exerttheir potentially lethal effects by specifically and potentlyblocking a variety of...
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Báo cáo Y học: Cloning and characterization of the mammalian-specific nicolin 1 gene (NICN1) encoding a nuclear 24 kDa protein doc
... Nuclear import of glycoconjugates is distinct from the classical NLS pathway. J. Cell Sci. 108, 1325–1332.SUPPLEMENTARY MATERIALThe following material is available from http://www.blackwell-science.com/products/journals/suppmat/EJB/EJB3232/EJB3232sm.htmFig. ... wasadded.Biosynthetic labeling of transfected cells and immunoprecipitation of cell extractsCOS-1 cells were cultured in DMEM supplemented with10% fetal calf serum, 50 UÆmL)1penicillin and 50 mgÆmL)1streptomycin ... Hassan Y. Naim2 and Tosso Leeb11Institute of Animal Breeding and Genetics and 2Institute of Physiological Chemistry, School of Veterinary Medicine Hannover;3Institute of Molecular Biology,...
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Tài liệu Báo cáo khoa học: Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum) ppt
... by immunofluorescence labelling and confocal microscopyLocalization of PsCIPK and PsCBL was analysed byimmunofluorescence labelling of tobacco BY2 cells fol-lowed by confocal microscopy. Cell ... partiallycloned by PCR and then followed by isolation of full-lengthclones by library screening. For partial cloning of pea CIPK(PsCIPK) or pea CBL (PsCBL), all the known sequences of AtCIPK ... and confocal microscopyExponentially growing tobacco BY2 suspension cells werefixed in 4% formaldehyde and permeabilized by cellulase and layered onto poly L- lysine-coated cover slips. The cellswere treated...
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Tài liệu Báo cáo khóa học: Cloning and characterization of two distinct isoforms of rainbow trout heat shock factor 1 ppt
... in a total volume of 50 lL with KOD-Plus-DNAPolymerase (Toyobo) using 1 lL of the plasmid RTG-2cDNA library described above as the template. The PCRconsisted of one initiation cycle of 2 min ... digoxigenin;HA, hemagglutinin; EMSA, electrophoretic mobility shift assay;EGS, ethylene glycol bis (succinimidyl succinate); DBD,DNA binding domain; HR, hydrophobic heptad repeat;NLS, nuclear localization ... with RNAisolated from RTG-2 cells as described below. Approxi-mately 1.2 · 106plaques were screened at 2 · 105plaquesper 140 · 100-mm plate by hybridization of duplicatenitrocellulose membranes...
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Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx
... catalysis; X-ray structure.Chitin, b-(1,4)-linked poly (N-acetylglucosamine), is one of the most abundant polymers in nature. It is a majorcomponent of the cell wall of yeast and other fungi, and ... Heveabrasiliensis. It is located in so-called lutoid bodies, which arelow pH vacuolar organelles filled with hydrolytic enzymes and lectins [7]. These lutoid bodies are believed to play animportant ... Asp125Ala/Tyr183Phe doublemutants, and the Asp125Ala/Glu127Ala/Tyr183Phe triplemutant. The single Asp125Ala and Glu127Ala mutants hadapproximately 2% of the wild-type hevamine activity.Mutants...
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Báo cáo khoa học: Cloning and characterization of the genes encoding toxic lectins in mistletoe (Viscum album L) pot
... MLIII,respectively.Materials and methodsPlant materialYoung leaves of mistletoe (Viscum album L) were harvestedat the end of May from plants growing on a poplar tree(Populus alba L) in the Poltavskaya ... the MLpvariants and ricinThe MLp variants have a high overall sequence similarityto structurally and biologically related ricin D [26]. TheA-chains of ML1p, ML2p and ML3p have 40(54–57%) of identity ... ML2p and ML3p toMLII and MLIII. The ML1p, ML2p, ML3p and ML3.1pA-chains were expressed in E. coli in soluble and biologic-ally active form. The recombinant ML3p A-chain wasrecognized by the...
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Báo cáo Y học: Cloning and expression of sterol D14-reductase from bovine liver potx
... N-terminus of the protein (Fig. 3).Cellular localization. The c ellular localization of myc-tag-D14-SR was examined in transiently t ransfected COS-7cells. Double immuno¯uorescence analysis of cells ... Biochemical Sciences ÔA. Rossi FanelliÕ, UniversitaÁÔLa SapienzaÕ Roma, Italy;4Department of Biochemical Sciences and Molecular Biotechnology, University of Perugia, ItalyBiosynthesis of cholesterol ... Agnese Della Fazia4 and Giuseppe Servillo41Department of Internal Medicine, University of Perugia, Italy;2Department of Pharmacological Sciences, University of Milan,Italy;3Department of Biochemical...
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Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc
... tohomogeneity by HPLC and digested with t rypsin. Thetryptic peptides were separated by HPLC and some of themwere totally or partially analyzed by Edman sequencing and/ or by MALDI-TOF mass spectrometry ... protein of Brassica, spinach, barley and A. thaliana,PR1ofPhaseolus and MHF9 of A. thaliana.These proteins b elong to the 2Cys-Prx subfamily. A ll plan t2Cys-Prx proteins, except BAS1 of barley, ... 106cellsámL)1.Puri®cation of Ch-Prx1Puri®cation of the native protein from Chlamydomonascells. Chlamydomonas CW15 ce lls were grown to a celldensity > 5 ´ 106cells pe r mL. Ce lls were pelleted,resuspended...
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Báo cáo Y học: Expression and characterization of recombinant vitamin K-dependent c-glutamyl carboxylase from an invertebrate, Conus textile doc
... and 20%(v/v) glycerol and sonicated. COS7 cells (5 · 106cells) werewashed with NaCl/Pi, trypsinized and collected in NaCl/Pi(pH 7.2) containing 20% glycerol and 1 · PIC. Cells werehomogenized ... carboxylase activity and vitamin K-dependent epoxidase activity. The recombinant carboxylasehasamolecularmassof 130 kDa. The recombinant Conuscarboxylase carboxylated Phe-Leu-Glu-Glu-Leu and ... source of Conus carboxylase free of endogenous carboxylase and carboxylase substrates.Molecular mass analysis of the recombinantConusvitamin K-dependent carboxylaseThe molecular mass of the...
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