... studying the properties of these receptors todetermine the level of conservation of their function and theirrole in the complex development of this parasite. One of these, SmRXR, has been the ... of protein synthesis characteristic of these stages. Interest-ingly, the levels of detected protein vary considerablythroughout the life cycle, in a manner different from the mRNA levels. The ... out by removing aliquots of the polymerase reaction every four cycles starting at the eighth cycle, dot-blotting the samples on to a charged nylonmembrane and hybridizing exactly as previously...