Báo cáo y học: "enetic variability and population structure of endangered Panax ginseng in the Russian Primorye" doc

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... staining of cystatin F and ER.(A) Cystatin F was stained with a human cystatin F specific polyclonal antiserum and aFITC labelled secondary antibody (green sig-nal). (B) Following the cystatin F staining, ... partially explain the restricted and regulated cystatin F expression. The reasons why cystatin F in U937 cells is transported in a different way than the major type 2 cystatin of mammaliantissues, cystatin ... cystatin F in 2- or 4-dayculture medium). By contrast, U937 cells contained only3–4% of the related inhibitor, cystatin C. Cystatin F purifiedfrom lysates of U937 cells showed three major forms...

... Christian-Albrechts-University of Kiel, GermanyTo gain insight in the subcellular localization of tumor necrosis factor receptor-associated factor (TRAF4) weanalyzed GFP chimeras of full-length TRAF4 and variousdeletion ... Intracellular localization and transcriptional regulation of tumor necrosis factor (TNF) receptor-associated factor 4 (TRAF4) Heike Glauner1, Daniela Siegmund1, ... to H. Wajant, Institute of Cell Biology and Immunology, University of Stuttgart, Allmandring 31,70569 Stuttgart, Germany.Fax: + 49 711 685 748 4, Tel.: + 49 711 685 744 6,E-mail: harald.wajant@po.uni-stuttgart.deAbbreviations:...

... Processing, stability, and kinetic parameters of C5a peptidase from Streptococcus pyogenes Elizabeth T. Anderson1, Michael G. Wetherell1, Laurie ... by an exogenous proteasestreptopain.Proteolytic activity and thermal stability of the C5a peptidase The earlier reported highly restricted substrate specificity of C5a peptidase for human C5a ... temperature from 5 °Cto40°Cresultedina10-foldincrease of the relative proteolytic activity of C5a peptidase Fig. 2. Incubation of S512A C5a peptidase precursor with wild-type C5a peptidase (panel A) and...

... charge.In summary, we present evidence for a very strongsimilarity of HCII from human serum and its r ecombinantcounterpart from CHO cells with respect to tyrosine sulfation and N-glycosylation. The ... inhibitors of tyrosine s ulfation and N-glycosylation under conditions that allowed partialinhibition of these modifications. A fter dialysis, the condi-tioned medium was fractionated on heparin Sepharose ... (lgper106 cells per day) wascalculated.Treatment of HCII expressing CHO cells with inhibitors of N-glycosylation and tyrosine sulfation Recombinant CHO cells producing human HCII weregrown...

... http://arthritis-research.com/content/9/4/R84Page 5 of 10(page number not for citation purposes)Figure 2Adhesion assay and number and morphology of colony forming units (CFU)Adhesion assay and number and morphology of colony forming units ... articleReduced number and impaired function of circulating progenitor cells in patients with systemic lupus erythematosusJan Renier AJ Moonen1, Karina de Leeuw2, Xavier J Gallego Y van Seijen1, ... 7 of 10(page number not for citation purposes)The decrease in the number of circulating progenitor cells in patients with SLE is similarly seen in other diseases with vas-cular inflammatory...

... 29:162http://www.jeccr.com/content/29/1/162Page 8 of 8 RESEA R C H Open Access XAF1 expression and regulatory effects of somatostatin on XAF1 in prostate cancer cellsZhaoquan Xing1†, Zunlin Zhou2†, Rong Yu3,4, ... occur during the development of prostate cancer. It is interesting to evaluate the potential regulatory effects of somatostatin on XAF1 expression during the development of prostate cancer cells.Methods: ... 2001,28:836-846.doi:10.1186/1756-9966-29-162Cite this article as: Xing et al.: XAF1 expression and regulatory effects of somatostatin on XAF1 in prostate cancer cells. Journal of Experimental &Clinical Cancer Research 2010 29:162.Submit...

... article as: Ling et al.: Marked disability and high use of nonsteroidal antiinflammatory drugs associated with knee osteoarthritis in rural China: a cross-sectional population-based survey. ArthritisResearch ... interviewer-based questionnaire, evaluating knee pain and associated disability, analgesia, and health services use, and obtained bilateral standardized weight-bearing knee ra diographs.Results: Of the ... verydissimilar.ConclusionsGiven the high prevalence of knee pain in Wuchuan with an associated increased physical disability, NSAIDs, and health services use, we suggest that knee pain and symptomatic...

... 5 of 15 RESEARCH ARTICLE Open AccessRelative percentage and zonal distribution of mesenchymal progenitor cells in human osteoarthritic and normal cartilageDavid Pretzel1,2*, Stefanie Linss1, ... furtheranalyzed. Zonal distribution of CD166+ mesenchymal progenitor cells in the cartilage matrixCD166+chondrocytes were almost exclusively located in the superficial and middle zones of OA and normal ... (Figures 6 and 7).DiscussionAbundance of mesenchymal progenitor cells in osteoarthritis and normal cartilageHigh percentages of C D105+/CD166+ cells wereobserved by FACS in OA /normal cartilage...

... RESEARCH Open Access Chemical fingerprinting and quantitative analysis of a Panax notoginseng preparation using HPLC-UV and HPLC-MSHong Yao, Peiying Shi, Qing Shao, Xiaohui Fan*AbstractBackground: ... ion spray voltage, -4.5 kV; sheath gas(N2) at a flow rate of 60 arbitrary units; auxiliary gas(N2) at a flow rate of 20 arbitrary units; capillary tem-perature, 350°C; capillary voltage , ... of TCM Injections SFDA, Beijing; 2000.doi:10.1186/1749-8546-6-9Cite this article as: Yao et al.: Chemical fingerprinting and quantitative analysis of a Panax notoginseng preparation using HPLC-UV...

... genetic polymorphism and population structures of wild P. ginseng with the AFLP method to (1) estimate the level of genetic diversity in the P. g inse ng populations in the Russian Primorsky Krai, ... estimate the level of genetic diversity in the P. gin sen g populations in the Russian Primorsky Krai, (2) calculate the distribu-tion of variability within a population and among popula-tions and ... calculate the distribution of variability within a population and among populations and (3) examine the genetic relationship between the populations.Methods: Genetic variability and population structure...

... http://www.tbiomed.com/content/6/1/30Page 10 of 16(page number not for citation purposes) Theoretical Biology and MedicalModellingResearch A modeling and simulation study of siderophore mediated antagonism in dual-species biofilmsHermann ... the simulation, albeit at a decreased rate.The simulation stops at t =10d,where,asindicatedalready before, all the non-chelator is accumulated in a single colony that is not yet notably invaded ... hisimplies that the total amount of biomass in the system isbounded by the initial amount of iron and biomass in the system and that biomass is in fact eventuallydecreasing. More specifically we haveNtx Y YRt...

... circulating monocyte and dendritic cell subsets in patients with sepsis at baseline and at day 28 compared with healthy controls. Monocyte and dendritic cell subsets were stained and gated as described ... of proinflammatory cytokines and intracellular cytokine staining in response to lipopolysaccharide and lipoteichoic acid was impaired in monocyte subsets and MDCs, whereas IL-10secretion was increased. ... transduction may play a role in monocyte and DC deactivation in sepsis [48].ConclusionsWe demonstrate a profound and sustained functional impair-ment of all circulating monocyte and DC subsets in patients with...

... transportNIPA-like domain containing 1, Npal1 UnknownTyrosine kinase, Txk Tyrosine protein kinase, transcription factorTyrosine-protein kinease, Tec Tyrosine protein kinase, signalingCMSat4 None NA *The functionality ... chromosome number in the Indian muntjac has captured the interest of geneticists for anumber of years [2]. The rarity of such a karyotype amongmammals suggests that the extant Indian muntjac genomeformed ... fusion sites. ConclusionOur studies help to provide a better understanding of the likely evolutionary history of the Indian muntjac karyotype aswell as insights into the paradoxical finding that...