... study on RA patients [13].The coculture supernatants were screened by ELISA orCBA for the concentration of the T cell-produced cytokinesIFN-γ, IL-2, TNF, IL-17, IL-10, IL-13 and IL-4. In all patients, IFN-γ ... involume of synovial fluid; and a local production of proinflam-matory cytokines. Interleukin-17 (IL-17) is a T cell-derivedcytokine with similar proinflammatory properties to IL-1 and TNF in ... werestained with anti-CD3-FITC (clone SK7), anti-CD4-PerCP(SK3) and anti-CD25-APC (2A3) (all from Becton Dickin-son [BD], Franklin Lakes, NJ, USA). The stained cells wereanalyzed by flow cytometry on...