... Veterinary Medicine of SichuanAgricultural University, Ya’an, Sichuan, 625014, China.2Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Ya’an, Sichuan,625014, China.3Epizootic ... (LB) agar medium containing 50μg/mL kanamycin, and were incubated overnight at 37°C. 200 m L LB medium containing 50 μg/mL kanamycinwas inoculated with a freshly grown colony containing the ... disease outbreaks. Clinical and laboratory tests have confirmed that the attenuatedDEV vaccine is an effective biological agents for the pre-vention and control of DVE, and the monitoring of DEV-specific...