... Ltd., for kind anal-ysis with microarrays, and Dr. Sumio Sugano, Human Genome Center, Institute of Medical Science, University of Tokyo, for kindly providing a mouse spleen cDNA library. We ... wasused for the analysis. Sample labeling and processing wereperformed according to the manufacturer's protocol. In brief,double-stranded complementary DNA was synthesized, and biotinylated ... genemanipulation.Preparation of total RNA and poly (A)+ RNA from jointsAfter removal of the skin and muscle, portions of the leg con-taining the knee, ankle, and finger joints were rapidly...