... Lin,S J.,Kim,D W.,Ryugo ,Y. ,Wakagi,T.&Matsuzawa,H.(1997) Increase of the protease activity of aqualysin I, a thermo-stable serine protease, by replacing Asn 219 near the catalyticresidue Ser ... 17448–17453.18. Feller, G., Payan, F., Theys, F., Qian, M., Haser, R. & Gerday, C.(1994) Stability and structural analysis of a- amylase from theantarctic psychrophile Alteromonas haloplanctis. Eur. J. ... 5¢-ATGTTAAAGAAAGTATTAAGTTGTTG-TATTGCAGC-3¢ and5¢-AAAGTTTGCTTGGAGCGTCAAGCC-ACTGTAAGCCG-3¢, cloned into the expression vector pBAD-TOPO,transformed into the E.coliTop10 strain and grown on LBagar...