Báo cáo khoa học: "Echocardiography and assessing fluid responsiveness: acoustic quantification again into the picture" pptx

... toassess fluid responsiveness. First, despite the carefulimplementation of passive leg raising, a small shift ordisplacement of the TOE probes in relation to the LV shortaxis could distort the ... at the bedside.Competing interests The author(s) declare that they have no competing interests.AcknowledgementsWork conducted by the authors is supported by an unrestricted grantfrom the ... noninvasive and dynamic manner. The great strength of this tool lies in its power to addsupplementary information regarding contractility and afterloading conditions in a direct, comprehensible and physiological...

Báo cáo khoa học: Molecular and functional characterization of novel CRFR1 isoforms from the skin pptx

... whether these messagesare translatable. In the second step, we characterized theircoupling to different signaling pathways and their modu-latory role on the CRFR1a activity.Materials and ... expressionTo verify that the constructs produce proteins of the expected masses, we attached the V5 epitope to the Cterminus of the CRFR1 isoforms (Fig. 1C). The predictedmasses of the isoforms without/with ... E27(5¢-GGTAGTGCACCTTGCTTTTTTTCTCTCCCCA-3¢) and attached to another fragment of CRF receptor in the ﬁnal PCR by primers E3 and E11.To attach V5 epitope to the CRFR1a,g,h2ande2isoforms we ampliﬁed the corresponding DNA...

Báo cáo khoa học: " Echocardiography and electrocardiography as means to evaluate potential performance in horses" docx

... index to evaluate the heart size in the horse and that it should not be considered as a tool for assessing potential performance. On the other hand, there are currentlymore suitable and accurate ... at least in the final part of the PQinterval and/ or in the initial part of the ST interval, the ventricular depolarization could start and continue withcurrents that conceal each other, without ... toevaluate the heart size in the horse. On the other hand,there are currently more suitable and accurate proceduressuch as echocardiography that allow performing a clearanatomical evaluation and...

Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of ﬂavoproteins: electron transfer through the nitric oxide synthase ﬂavoprotein domain pdf

... FAD and FMN. The two domains are separated by aCaM-binding motif. During catalysis, NADPH-derived electrons transfer into the FAD and FMNin each NOS subunit and then on to the ferric hemein the ... toreduce the heme (Fig. 5). However, the lowest possiblerates for the FMN subdomain dissociation step (koff)in the CaM-bound eNOS and nNOS are  1 and 20 s)1, respectively [58] (Table 2), and these ... hinge domains The composition of the two hinges that connect the FMN subdomain in NOS enzymes (H1 and H2 inFig. 5) deﬁnes the allowable movements of the FMNsubdomain and thus controls the FMN–NOSoxy...

Tài liệu Báo cáo khoa học: Gas6 and protein S Vitamin K-dependent ligands for the Axl receptor tyrosine kinase subfamily pptx

... juxtapose itselfagainst and interact with the membrane, thus enabling the SHBG domain to bind to the receptor on either the same or another cell.Axl, Sky and Mer in cancer The transforming activity ... binding to and activa-tion of the receptor tyrosine kinases Axl, Sky and Mer. Furthermore, ahallmark of the Gas6–Axl system is the unique ability of Gas6 and pro-tein S to tether their non ... expands the boundaries of signalling mediators, and it is thereforenot unlikely that Sky and Mer could be involved inTable 1. Afﬁnities of Gas6 and protein S for Axl, Sky and Mer RTKs within and...

Tài liệu Báo cáo khoa học: "Fast and Robust Part-of-Speech Tagging Using Dynamic Model Selection" pptx

... two other state-of- the- art systems, the Stanford tagger (Toutanova etal., 2003) and the SVMTool (Giménez and Màrquez,2004). Both systems are trained with the same train-ing data and ... domain and the other is optimized for a domain specific to the training data. During decoding, we dynamically se-lect one of the models by measuring similarities be-tween input sentences and the ... networks, and showed the best contemporary results. Giménez and Màrquez (2004) used one-pass, left-to-right and right-to-left combined tagging algorithm and achieved near state-of -the- art...

Tài liệu Báo cáo khoa học: Characterization and mode of action of an exopolygalacturonase from the hyperthermophilic bacterium Thermotoga maritima doc

... derived from the maximum velocity(V), divided by the molar concentration of the enzyme(e0, included in Vmax). R and T are the gas constant and the temperature (in Kelvin), respectively. The values ... mappingOn the basis of the assumptions of Hiromi [30] that the intrinsic rate of hydrolysis (kint) in the productivecomplex is independent of the length of the substrate,Km and Vmaxwere ... Microbiology, Wageningen University, the Netherlands2 Laboratory of Food Chemistry, Wageningen University, the NetherlandsPectin is a complex polysaccharide present in the cellwall of higher plants,...

Báo cáo khoa học: Transthyretin and familial amyloidotic polyneuropathy Recent progress in understanding the molecular mechanism of neurodegeneration pdf

... showing the location ofamyloidogenic mutations and position of b-strands. (A) The struc-ture of the polypeptide backbone of the two chains (purple and blue) is shown along with the location of the ... occurs in the region between residues 45 and 58. This regioncontains the C strand, CD loop, and D strand whichare located at the edge of each monomer [97]. It hasbeen suggested that the amyloidogenic ... sixb-strands instead of eight, with the C and D strands and the intervening loop forming a large loop, expos-ing some hydrophobic residues in this region that arenormally buried on the inside of the...

Báo cáo khoa học: Structural and functional roles for b-strand 7 in the a-crystallin domain of p26, a polydisperse small heat shock protein from Artemia franciscana pdf

... b-strand 7,with mutations R110G and F112R directly in the dimer–dimer interface. As a result of the spatial dis-position of monomers and their b-strand elements in the a-crystallin domain, the ... study are notlocated in either of these strands and they are not con-sidered further.As the basic p26 oligomer building block, dimersinteract to form tetramers, the next level of struc-ture. ... in the presence of dithiothreitol, and solution turbidity was measured at 400 nm. The p26 variants tested areindicated in the ﬁgure and they appear in the same order in each histogram group. The...

Báo cáo khoa học: Characterization and regulation of a bacterial sugar phosphatase of the haloalkanoate dehalogenase ppt

... and ARA440 (Table 1) were used and introduced unique NdeI and XhoI restriction sites at the 5¢ and 3¢ end, respectively, and the resulting PCR product was inserted into pET30adigested with the ... 0.4 and 52.2 ± 0.5 min,respectively), indicating both the stability of the strainbearing the in-frame deletion and the fact that the AraL enzyme is not involved in l-arabinose utilization. The ... monophosphates and, in particu-lar, UMP and GMP. The structure of NagD has beendetermined and the occurrence of NagD in the contextof the nagBACD operon indicated its involvement in the recycling...

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