... cloning of the Tat mRNA sequences
To study the mechanism of protein synthesis from the Tat
mRNA, we cloned the Tat1 and Tat2 sequences, the two
major forms of Tat mRNA [43], by means of a PCR-DNA
reconstruction ... of 18
(page number not for citation purposes)
Retrovirology
Open Access
Research
Mechanism of HIV-1 Tat RNA translation and its a...
... to the binding of the metal to the N-lobe,
although the binding favorsthe C-lobe thermodynamically as
judged by
1
H NMR. The more open conformation of the N-
lobe may facilitate Yb
3+
ion binding ... iron
requirement and subsequently express much higher Tf
receptors [39]. Then the uptake of Yb-bound Tf by the tumor
cells might be much higher than the normal cells...
... bifunctional ligand, has been neglected. The
consistency of the K
d
values resulting from the evaluation of
the equilibrium response signals and those obtained by
evaluating the first step in the association ... previously [13,35]. The K
d
determined for factor X
is consistent with the value determined by McDonald
et al.[9].
The effect of the serine protease dom...
... diag-
nosis of infection with human immunodeficiency virus type 1
(HIV-1) by detection of antibody IgGs to pol and gag proteins
of HIV-1 and p24 antigen of HIV-1 in urine, saliva, and/ or
serum ... an
indicator of subject's state of health[26]. HIV-1 RNA/ DNA
and CD4 mRNA detected in the fecal specimens of HIV-1
infected subjects could be the result e...
... when the ALT level was maximum.
Clinical parameter and pregnancy
The maximum duration of viremia was 42 days and 36
days, fecal shedding 28 days and 19 days and IgM anti
HEV 46 days and 49 days ... from the study because
of lack of desired follow up. The mean age of the
patients was 29.2 ± 8.92 years (range: 11 to 54 years)
and there were 39 males and 21 female...
... normal
function of AEC by a variety of mechanisms. Some of
these include decreases in the level of exhaled NO,
enhanced release of pro-inflammatory cytokines, and
inhibition of the airway repair process ... prostaglandins
and a variety of cytokines [7]. In the lungs and skin of
smokers mast cells increase in absolute numbers and
smoking may be associated with...
... lymphocytes and monocytes. We have previously shown that
IκB-αS32/36A, a proteolysis-resistant inhibitor of NF-κB, potently inhibits the growth of HIV-1 and
SIVmac239 in cell cultures and in the ... vivo.
Background
HIV-1 infection is characterized by a long period of clini-
cal latency followed by the development of acquired
immunodeficiency syndrome, AIDS. During...
... isoforms of LIF mRNA. Expression of LIF by myofibroblasts and of its receptor
by adjacent cells suggests a potential LIF paracrine loop in human liver that may play a role in the
regulation of ... of endothelial cell and/ or hepatocyte labeling. Immunohistochemistry,
flow cytometry and STAT-3 phosphorylation assays did not provide evidence for LIF receptor
expression b...
... homozygous
and TIRAP/Mal heterozygous. Of 29 TLR4 heterozygous
mutation carriers, 24 were TIRAP/Mal heterozygous and
5 homozygous. The distribution of SNPs in the studied
patients and the cohort of ... JMWvdM and KZ contributed to conception
and design of the study. Statistical analysis was performed by OK and EJG-B.
RRS headed the project, supervised and con...
... phenol-chloroform extraction steps as prescribed
by the manufacturer.
Microarray analysis of gene-expression profiles
Microarrays were generated by spotting the Compugen 19 K
human oligo library, made by ... respectively, and
hybridized to the arrays using protocols established by the
Patrick O. Brown Laboratory [59]. Array images and data
were obtained and processed u...