Báo cáo khoa học: "Endotracheal tubes and imposed work of breathing: what should we do about it, if anything" pps
Báo cáo khoa học: "Endotracheal tubes and imposed work of breathing: what should we do about it, if anything" pps
... Bransonventilation. Recent work suggests that the work of breathingpostextubation may actually increase compared with work of breathing through the ET tube, raising questions about theconclusion of the study ... advantages of ATC over PSV are reduced work of breathing as patient demand varies, preservation of anormal, variable breathing pattern, and improved synchrony. Ina recent trial of spontaneous ... Maeda and coworkers is the role of expiratory compensation. One distinct difference in theoperation of the Drager Evita 4 and Puritan Bennett 840 isthat Evita 4 provides both inspiratory and...
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Tài liệu Báo cáo khoa học: Helix mobility and recognition function of the rat thyroid transcription factor 1 homeodomain – hints from 15N-NMR relaxation studies pdf
... graphs of Fig. 3, and the corresponding numerical values are given insupplementary Table S4. Linear correlations betweenJ(0) and J(xN) and between J(0) and J(0.87xH) for theTTF-1 HD were ... dis-tribution of the energy between the overall tumbling and the internal mobility, and is indicative of thedegree of internal restraint of each15N–1H vectormotion. In Fig. 4, most of the points ... strong net charge of the molecule (+15) should prevent significant association. Addressing this issueadequately, however, is beyond the scope of the cur-rent work, and should be done in detail...
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Tài liệu Báo cáo khoa học: Purification and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx
... hydroxylation of Pro, Val or Lys, c-carboxylation of Glu, glycosylation of Ser or Thr, bromination of Trp, sulfation of Tyr, cyclization of N-terminal Gln, and epimerization of several different residues. ... 4.70 p.p.m. and a spectral window of 6000 Hz, as described previously [22].Spectra were processed with topspin or xwinnmr soft-ware. Phase-shifted sine-squared window functions wereapplied ... both samples of conomarphin and l-Phe13-conomarphin were recorded at 300 K and pH 3.Restraint set generationAn initial survey of distance constraints was performed ona series of NOESY spectra...
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Tài liệu Báo cáo khoa học: The antibacterial and antifungal properties of trappin-2 (pre-elafin) do not depend on its protease inhibitory function pptx
... reaching 92%after 6 h and 98% after 18 h (Fig. 5).Effect of NaCl and heparin on the antibacterial and antifungal activities of trappin-2The antimicrobial and antifungal activities of trappin-2are ... properties of trappin-2involve the cationic nature of the molecule, as assessedfrom the salt and heparin dependence of the antimicro-bial and antifungal effects.ResultsAntimicrobial effects of recombinant ... manner, except forK. pneumoniae and H. influenzae, which were less sensi-tive at high doses than at low doses of approximately5 lm (Figs 2 and 3). The minimum effective doseP. aeruginosa ATCC 278534050607080901000...
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Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc
... concentration of Rep245 pre-pared in 10 mm sodium phosphate (pH 7.4) was 5 lm. CDspectra were recorded at 60, 70 and 80 °C between 190 and 260 nm with a step increase of 0.2 nm, and a bandwidth of 1 ... increases were seen to influence the size of DNAproducts: small amounts of DNA primers between 16 and 20 nucleotides in size were synthesized at 30 °C; inthe temperature range between 40 and 65 ... DNArecognition and binding and is positioned left of the active site [14].Rep245 is both capable of de novo synthesis of DNA primers and of elongating them. Long DNAextension products were observed...
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Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx
... electrophoresis of the PCR product indicated thepresence of two distinct bands between 1000 and 1100 bp of roughly the same extent (not shown). Clo-ning and nucleotide sequencing of these two ... PCRmutagenesis reaction. Mutants were sequenced to verifyincorporation of the desired modification and to ensure theabsence of random mutations. The mutagenized plasmidswere transformed into P. pastoris ... inmedia containing isoform I. Both isoforms werealso expressed intracellularly, as evidenced by theappearance of protein bands of the expected sizeupon SDS ⁄ PAGE analysis of the crude extractsprepared...
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Tài liệu Báo cáo khoa học: Biochemical characterization and inhibitor discovery of shikimate dehydrogenase from Helicobacter pylori docx
... shikimate, kcat of 7.1 s)1 and Km of 0.182 mm toward NADP, kcat of 5.2 s)1 and Km of 2.9 mm toward NAD. The optimum pH of theenzyme activity is between 8.0 and 9.0, and the optimum temperature ... structures[26].Moreover, we have investigated the catalytic proper-ties of HpSDH and the effects of pH and temperatureon HpSDH. The results show that HpSDH has a kcat of 7.7 ± 0.9 s)1, Km of 0.148 ± 0.028 mm and kcat⁄ ... and kcat⁄ Km of 5.2 · 104m)1Æs)1toward shikimate, and akcat of 7.1 ± 0.7 s)1, Km of 0.182 ± 0.027 mm and kcat⁄ Km of 3.9 · 104m)1Æs)1toward NADP. Differentfrom AroE of E. coli...
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Tài liệu Báo cáo khoa học: Receptor association and tyrosine phosphorylation of S6 kinases pdf
... four dis-tinct bands on a SDS ⁄ PAGE gel due to multiple phos-phorylation. The tyrosine phosphorylated bands of S6K overlap with two of the activated and slowermigrating bands. We have shown ... Stephens7, Andrew West7 and Ivan T. Gout2,31 Ludwig Institute for Cancer Research, London, UK2 Department of Biochemistry and Molecular Biology, University College London, UK3 The Institute of Molecular ... homologyin their kinase domains and have a similar mode of activation [1].There are two isoforms of S6 kinase, S6K1 and 2.Both have highly homologous kinase and kinaseextension domains flanked by...
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Tài liệu Báo cáo khoa học: Design, structure and biological activity of b-turn peptides of CD2 protein for inhibition of T-cell adhesion ppt
... further dockingstudies with flexible docking.During flexible docking, the dihedrals of backbone of theligand were kept rigid, whereas the dihedrals of side chainwere allowed to rotate. After docking, ... defaultvalues of theAUTODOCKLamarckian genetic algorithm.First, a randomized rigid docking (blind docking) wasperformed and the conformers with lowest energy or insignificant clusters were chosen ... region of CD2 interacts with C D58. First, arandomized rigid docking (blind docking) was performed and the conformers with lowest energy or in significantclusters were chosen to perform further docking...
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Tài liệu Báo cáo khoa học: The structure and biological characteristics of the Spirochaeta aurantia outer membrane glycolipid LGLB pdf
... constants and 13C NMR chemical shifts.Anomeric configurations were deduced from the J1,2coupling constants and chemical shifts of H-1, C-1 and C-5 signals. The position of C-6 signals of uronic ... liberation of the FAMEs, the hydrolysates were neutralized with 0.46 g of silver carbonate and doped with 204.5 lg of tridecanoicacid (in n-pentanol) as an internal standard. The sampleswere centrifuged ... HSQC and HMBCspectra of compound 1. Labels illustrateassignment of the amide linkage between theamino group of Asp and the carboxyl group of GalA residue E.Scheme 1. The structures of o ligosaccharides...
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