Báo cáo khoa học: " In vitro analysis of expression vectors for DNA vaccination of horses: the effect of a Kozak sequence" docx

Báo cáo khoa học: In vitro analysis of the relationship between endonuclease and maturase activities in the bi-functional doc

... AnI19R5¢-AATTCATGAGGAGGTTTCTCTGTAACA-3¢;AnI19H5¢-AGCTTGTTACAG-AGAAACCTCCTCATG-3¢; AnI17R5¢-AATTCACGAGGAGGTTTCTCTGTACTA-3¢;AnI17H 5¢-AGCTTAGTACAGAGAAACCTCCTCGTG-3¢;AnI15R5¢-AATTCACAGGAGGTTTCTCT-GTCTA-3¢;AnI15H5¢-AGCTTAGACAGAGAAACCTCCTGTG-3¢. Each annealed ... toI-AniI before addition of the DNA substrate. To determinewhether the RNA substrate can inhibit the cleavage of prebound DNA substrate, a single-turnover DNA cleavagereaction was incubated at ... proteinwas saturated with the same amount of either substrate(Fig. 4) argues against the trivial explanation that only a fraction of protein was competent to bind DNA, leavinganother fraction...

báo cáo khoa học: " In vitro analysis of the cytotoxicity and the antimicrobial effect of four endodontic sealers" ppt

... tissues, and thus there are special demands for the sealing agent s [31]. The major task in reducing the bacterial load, concentrated in the apical region of the root canal, is achieved by the mechanical ... sea-lers have already been assessed for a possible antibacter-ial effect, most often measured against strains of E. faecalis [21-28].Baer and Maki [29] demonstrated that AH Plus andPulp Canal Sealer ... system and to establish a hermeticseal of the apical area of the root. To achieve this isdesirable to inhibit the growth of the microorganisms i.e. mainly bacteria remaining within the cleaned...

Báo cáo khoa học: " In vitro analysis of expression vectors for DNA vaccination of horses: the effect of a Kozak sequence" docx

... not for citation purposes)Acta Veterinaria ScandinavicaOpen AccessResearch In vitro analysis of expression vectors for DNA vaccination of horses: the effect of a Kozak sequenceGuðbjörg Ólafsdóttir1, ... 5'-GTATGCGGCCGCCACCATGAAGT-GGGTAACCTTTAT-3' with Kozak sequence and 5'-CGCTAGGATCCAATCAATGGTGATGGTGATGATG-3'.Taq DNA Polymerase (New England BioLabs) was used for PCR amplifications. The PCR products and DNA digested ... Littel-van den Hurk S van Drunen, Gerdts V, Loehr BI, Pontarollo R,Rankin R, Uwiera R, Babiuk LA: Recent advances in the use of DNA vaccines for the treatment of diseases of farmed ani-mals. Advanced...

Báo cáo khoa học: In vitro expansion of DNA triplet repeats with bulge binders and different DNA polymerases pdf

... DNA drug complex [33]. The signiﬁcant change in the band at 250 and 280 nmimplied an alteration in the DNA conformationbecause of an overall bending of the DNA backbone[33,35]. Both DDI- 1A and ... not affect DNA slippage (data notshown). Interestingly, there was a similar hierarchy of intensities for the three bands in the (AAT)3⁄ (ATT)5and (ATT)3⁄ (AAT)5systems (Fig. 1A, B), each ... con-formation [14]. In order to observe the conformational transitions of DNA directly and to eliminate drug interference, the CD spectra of native DNA and altered DNA, aftersubtracting the spectrum...

Báo cáo khoa học: In vitro embryonic developmental phosphorylation of the cellular nucleic acid binding protein by cAMP-dependent protein kinase, and its relevance for biochemical activities pdf

... Yasuda J, Mashiyama S, Makino R, Ohyama S, SekiyaT & Hayashi K (1995) Cloning and characterization of rat cellular nucleic acid binding protein (CNBP) cDNA. DNA Res 2, 45–49.13 Tomonaga T, Michelotti ... open La autoantigen(La)-bound form would, instead, allow translation. Cellsignals might in uence the affinities of La or CNBP for the 5¢ UTR, and the alternative binding of these pro-teins may ... function of CNBP.Fig. 1. (A) In silico analysis of zebrafish CNBP amino acid sequence. The amino acid sequence of zebrafish CNBP was analysed using the Prosite server. The amino acid residues involved...

Báo cáo khoa học: In vitro gamma-secretase cleavage of the Alzheimer’s amyloid precursor protein correlates to a subset of presenilin complexes and is inhibited by zinc potx

... mass of the CTFmigrating faster than C101-3FLAG as an a- CTF within27 Da of the calculated molecular mass. Finally the same line was used to predict the molecular mass of a third FLAG-reactive ... c-3FLAG standard. Finally, the pri-mer pairs forward, 5¢-GGGGGGCCATGGTGATGCTGAAGAAGAACAG-3¢ and reverse 3FLAG HindIII wereused to generate the plasmid e-3FLAG standard.Preparation of MC99-3FLAGEscherichia ... (Ab). The beta-siteAPP cleaving enzyme cleavage releases an APP ecto-domain leaving a 99-amino acid membrane spanningC-terminal fragment (CTF), C99. C99 then undergoesintramembranous cleavage...

Báo cáo khoa học: In silico analysis of the adenylation domains of the freestanding enzymes belonging to the eucaryotic nonribosomal peptide synthetase-like family pot

... cerevisiae,that the l -a- aminoadipate substrate could be adenyl-ated at the d-carboxylate rather than the a- carboxy-late and that the a- amino and a- carboxyl groups of the substrate bind at the bottom of the ... the A and T domains from NRPS systems and the C-terminal part contains a redox cofactor bindingsite for either pyrroloquinoline quinone (PQQ) orNADPH. In particular, AASDH, containing a PQQbinding ... of the pocket inter-acting with the Arg239 and Glu322 [14]. Analogousarrangement was proposed also for the binding of l -a- aminoadipate to the adenylation domain of the ACV synthetase from Penicillium...

Báo cáo khoa học: In vitro and in vivo self-cleavage of Streptococcus pneumoniae signal peptidase I pot

... thatinactivates the protein. The self-cleavage of LexA protein isimportant in the SOS response in bacteria [21–23]. In vitro self-cleavage was also observed in all investigated bacterialSPase ... itssubstrates. Therefore, the self-cleavage of SPase I is believedto inactivate the activity of the enzyme. In vivoself-cleavage of S. pneumoniaeSPase IAs the in vitro self-cleavage inactivates the ... pneumoniae Erademonstrated that approximately an equal amount of protein was loaded in each lane (Fig. 6D). The Era antibodywas selected because of its equally expression in all the growth phases of...

Báo cáo khoa học: In vitro selection and characterization of a stable subdomain of phosphoribosylanthranilate isomerase potx

... selectionmethods remains largely untested. We have investigated plasmid display as a suitable in vitro format by engineering a variant of phosphoribosylanth-ranilate isomerase (PRAI) that carried the FLAG epitope ... comparing the far-UV and near-UV CDspectra of PRAI and FLAG-PRAI in a buffer in which PRAI retains catalytic activity (Fig. 3). The spectra are effectively superimposable in both cases. The far-UV ... (SPR).On acquiring SPR data for FLAG-PRAI and trPRAIbinding to mAb M2, it became apparent that the latterdisplayed increased binding at any given concentration(Fig. 6). Afﬁnities for the antibody...

Báo cáo khoa học: In vitro characterization of a plastid terminal oxidase (PTOX) pot

... washing away the chelator byTable 1. Eﬀect of citrate, malate and NaCl on PTOX activity in vitro. Assays were performed in the presence of NADH, decyl-plastoqui-none and KCN as described in the ... concentration of NaCl was alsoincreased in a separate assay. PTOX activity is given as percentage of starting O2consumption (before addition of these compounds). The latter activity (approximately ... Gonzalez-Meler, M .A. , Siedow, J.N., Wagner, A. M. & Lambers, H. (2002) Role of sugars and organic acids in regulating the concentration and activity of the alternative oxidase in Poa annua roots. J....

Xem thêm