Báo cáo y học: "Psoriasin, one of several new proteins identified in nasal lavage fluid from allergic and non-allergic individuals using 2-dimensional gel electrophoresis and mass spectrometry" potx
Báo cáo y học: "Psoriasin, one of several new proteins identified in nasal lavage fluid from allergic and non-allergic individuals using 2-dimensional gel electrophoresis and mass spectrometry" potx
... purposes)Respiratory ResearchOpen AccessResearchPsoriasin, one of several new proteins identified in nasal lavage fluid from allergic and non -allergic individuals using 2-dimensional gel electrophoresis and ... 5.3 Proteins in bold are newly identified in NLF.Table 1: Identified proteins in nasal lavage fluid from allergic and non -allergic individuals. (Continued)Western blot analysis of NLF from ... Tagesson C, Lindahl M: Newly identified proteins in human nasal lavage fluid from non-smokers and smokers using two-dimensional gel electrophoresis and pep-tide mass fingerprinting. Proteomics...
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Báo cáo y học: "Systematic analysis of genome-wide fitness data in yeast reveals novel gene function and drug action" pot
... using fitnessdefect alone, a random forest using fitness defect alone, arandom forest using only the chemical structure similar-ity features, and a random forest using all features.The random ... mislocalization of Exo84,preventing the protein from performing its essential role in the exocyst.A second intriguing finding is our prediction of aninteraction between clozapine and both yeast Cox17 and its ... maximizing the potency of a given inhibitor, but invariably ignore factors critical forunderstanding drug action, including cell permeability and the potential interaction/inhibition of other proteins in...
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Báo cáo y học: "Abnormal networks of immune response-related molecules in bone marrow cells from patients with rheumatoid arthritis as revealed by DNA microarray analysis" ppsx
... reviewed and approved by the EthicalCommittee of Wakayama Medical University. B M fluid was intraoperatively obtained from the nine RApatients and the 10 OA patients while undergoingjoint arthroplasty. ... of data and analysis.RS performed mRNA expression analysis with microarrays. YS and KO treated and recruited the patients for this study, and analyzed the clinical data of the patients. TO and ... recommendations. Total RNA from the BMMCs was extracted by using the RNeasyMini Kit (Qiagen, Tokyo, Japan). A 3 μgsampleoftotal RNA was u sed for DNA microarray analysis by using GeneChip Human Genome...
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Báo cáo y học: "Distinct roles of CD4+ T cell subpopulations in retroviral immunity: lessons from the Friend virus mouse model" pptx
... Mosmann TR, Cherwinski H, Bond MW, Giedlin MA, Coffman RL: Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. J Immunol ... protectionagainst FVProtection from FV infection can be elicited by several different types of vaccines including killed and attenuatedviruses, viral proteins, peptides, and recombinant vacciniaor ... bybeing packaged in F-MuLV-encoded particles produced in cells co-infected by both viruses. FV infection induceslethal erythroleukemia in susceptible strains of mice[65]. Recovery from FV-induced...
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Tài liệu Báo cáo Y học: Functional analysis of DM64, an antimyotoxic protein with immunoglobulin-like structure from Didelphis marsupialis serum pdf
... first threedomains and are present in the inhibitory receptors of Fig. 6. Inhibition of in vitro cytotoxicity of myotoxins I or II by DM64.Cytotoxicity was analyzed in vitro using C2C12 skeletal ... residues phenylalanine and tyrosine typical of the Ig-fold are bold in each domain.Fig. 5. Inhibition of in vivo myotoxicity of myotoxins I or II by DM64.Groups of four mice were injected intramuscularly ... capacity of DM64 to inhibit the myotoxicity inducedby myotoxins I (Asp49) and II (Lys49) from B. asper venomwas analyzed in vivo and in vitro. DM64 effectively neut-ralized the myotoxic and cytotoxic...
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Báo cáo Y học: Defective translocation of a signal sequence mutant in a prlA4 suppressor strain of Escherichia coli doc
... regionsinto the lipid bilayer [55]. Possibly, the passage of exported proteins and the insertion of integral membrane proteins arefacilitated by distinct regions of the translocon. Indeed,studies ... Indeed,studies in eukaryotes indicated that signal sequences of secreted proteins and signal-anchor domains of membrane proteins are positioned differently in the Sec61p translocon[56]. Recently, a secY40 ... have indicated that the SecYEG complexfacilitates the insertion of inner-membrane proteins in addition to catalyzing protein translocation [51,52]. Mem-brane protein insertion and protein translocation...
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Báo cáo Y học: The role of hydrophobic active-site residues in substrate specificity and acyl transfer activity of penicillin acylase pdf
... and volume of the acyl binding pocket and thereby alter the binding mode and affinity of theenzyme for PAA and derivatives thereof, while maintainingthe hydrophobicity of the binding site.To ... (kcat/Km)forp-hydroxyphenylgly-cine methyl ester and phenylglycine methyl ester that weresimilar to the wild-type values, whereas the specificity con-stants for p-hydroxyphenylglycine amide and phenylglycineamide ... methyl ester or the amide asacyl donor.It appeared that 6-APA and 7-ADCA were able toefficiently deacylate the phenylglycyl- and p-hydroxyphe-nylglycyl-enzyme of bF24A, as indicated by, respectively,...
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Báo cáo Y học: Identification of novel membrane proteins by searching for patterns in hydropathy profiles potx
... Identification of novel membrane proteins by searching for patterns in hydropathy profilesJohn D. Clements and Rowena E. MartinSchool of Biochemistry and Molecular Biology, Australian National University, ... for new members of a functional class of mem-brane protein. It takes advantage of the highly conservedsecondary structure of functionally related membrane proteins. Such proteins typically have ... pattern of peaks in theirhydropathy profiles. (A) The hydropathy profile of a rat dopaminesymporter reveals a pair of peaks followed by a deep valley, then acluster of nine peaks. The peak, base and...
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Báo cáo Y học: Stepwise adaptations of citrate synthase to survival at life’s extremes From psychrophile to hyperthermophile pot
... suggesting a similar role in theseenzymes.Ionic interactions at the terminiIonic interactions may also be important for prevention of fraying of the N- and C-termini; several of these interactionsTable ... glyceraldehyde 3-phosphate dehydro-genase [42,43] and lumazine synthase [44]. Again, theelectrostatic strengthening of the intersubunit contacts is acommon theme in these proteins. Finally, ... of individual interactions tothermostability. This may be true for small monomeric proteins such as CheY and RnaseH, but for oligomeric proteins that make up > 85% of intracellular proteins, ...
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Báo cáo Y học: Detailed characterization of polydnavirus immunoevasive proteins in an endoparasitoid wasp doc
... respectively, instead of the calyxcells. Further, the expression of IEP proteins is notsynchronized with that of CkPDV capsid proteins; IEP proteins were observed approximately one day after theinitiation ... that capsid proteins of CkPDV are present in thecalyx cells (Fig. 5A). To examine whether IEPs are alsosynthesized in the calyx cells, immunocytochemical and in situ hybridization analyses were ... contains a single EGF-like motif and there is noother significant amino-acid sequence homology of these proteins except within the EGF-like motif. Other PDV genefamily containing the cysteine...
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