... Access
Research
Fertility of frozen-thawed stallion semen cannot be predicted by the
currently used laboratory methods
P Kuisma
1
, M Andersson
1
, E Koskinen
2
and T Katila*
1
Address:
1
Department of Clinical ... predicting the fertilizing capacity of semen.
The results of Exp. 1 could not be repeated, although the
stallions and methods used were p...
... as there is no clear
correlation between the number of amyloid plaque and
the severity of dementia [2–5].
It has recently been suggested that soluble Ab
species cause AD as the levels of these ... slows the oligomerization of Ab peptides by
binding to the peptides in their oligomeric state.
Figure 1B suggests that the number of PFD molecules
binding to one Ab oligomer...
... of ETA, or whether it involves the dual het-
erogeneous part of the toxin. Use of this approach will
provide novel insight(s) into the physiological signifi-
cance of the endosomal fragments of ... (intrinsic apoptotic effect).
Both pathways require the translocation of ETA into
the cytoplasm of toxin-treated cells. On the basis of
the reconstitution of the...
... numbers over the diagrams indicate the position of the corresponding nucleotides relative to the translation start site, and the numbers
in parentheses below the diagram show the position of the ... detection software as the cycle number at
which the fluorescence of the reporter dye crossed the
threshold in log-linear range of PCR. The copy numbers of
the...
... gener-
ated by clustalx [27] of some of these sequences
belonging to the proteinase K family, and the number-
ing in this alignment is used throughout the Results
and Discussion. In addition to the mesophilic ... of SPRK shows > 43%
sequence identity compared to the corresponding
region of the bacterial members in the PRK family
compared here (Fig. 2B). The only...
... orthophosphate after the hydrolysis of GTP. Completion of the Ran cycle is shown in the middle for Smad3
because the role of Ran has only been analyzed in detail in the case of Smad3. Cytoplasmic ... homology 1 domain of
all Smads binds to importin-b in the case of Smad3
and importin-a in the case of Smad4, while mutation
of the NLS blocks the ability of th...
... presence of
GalA was unequivocally confirmed by GC-MS analysis of
trimethylsilil ethers of methyl glycosides liberated from
lipid A by methanolysis (Fig. 1). The
31
P-NMR spectrum
of the crude ... are
components of acyloxyacyl residues. The predicted ions for
the third type of oxonium ions composed of DAG and
two amide acyl residues have not been registered, due to...
... cDNA of LEPRb,
phosphorylation of STAT1, STAT3, STAT5 and the
ERK kinases was induced by leptin. The weak
response of STAT5 to leptin compared to that elicited
by GH can be partially explained by ... Bahrenberg G, Behrmann I, Barthel A, Hekerman P,
Heinrich PC, Joost HG & Becker W (2002) Identifica-
tion of the critical sequence elements in the cytoplasmic
domain o...
... France),
quantified by the measurement of the peak area and referred
to known quantities of pure peptides used as standards.
Labeling of the toxin
The labeling was carried out in 37 lL of 400 m
M
Tris/HCl
buffer ... and used within a
month after labeling.
For iodination of the peptide using nonlabeled iodine, the
protocol was as described above, except that 200 lgof...
... affect the stereoselectivity of
epoxidation. The presence of the anionic lipid PtdSer
increased the formation of DE2 twice as much as that of
DE1. Even the relatively small portion of the negatively
charged ... stereoselectivity of epoxidation
The stereoselectivity o f the e poxidation reaction can be
demonstrated by the ratio of the formation rates o f...