Báo cáo khoa học: "Disintegration of Dung Pats from Cattle Treated with the Ivermectin Anthelmintic Bolus, or the Biocontrol Agent Duddingtonia ﬂagrans" ppsx

... 2003Disintegration of Dung Pats from Cattle Treated with the Ivermectin Anthelmintic Bolus, or the Biocontrol Agent Duddingtonia ﬂagransBy S O. Dimander, J. Höglund and P. J. WallerDepartment of Parasitology ... dung degradation. The aim of the present 3-year plot trial was tocompare the decomposition rate of uniform, ar-tiﬁcial dung pats derived from 3 groups of cat-tle that were either treated with ... or in the organic matter contentbetween dung pats from cattle treated with the Disintegration of Cattle Dung Pats 177Acta vet. scand. vol. 44 no. 3-4, 2003 the precipitation during the summer...

Báo cáo khoa học: " Evaluation of furosemide regimens in neonates treated with extracorporeal membrane oxygenation" doc

... vasopressor score over the observation period. More-over, the number of patients requiring inotropic supportdecreased during the study period.Renal function of the patients studied was within the ... newborns treated with ECMO. In our unit, continuous IV furosemide ther-apy was used in 78% of the neonates treated with ECMO. The dosing schedule of continuous IV furosemide in neonates treated ... performed at the age of five years according to the nationwide standardised evaluation of ECMO patients in The Netherlands to evaluate hearing loss as a sign of furosemidetoxicity (among other...

Tài liệu Báo cáo khoa học: Properties of ecdysteroid receptors from diverse insect species in a heterologous cell culture system – a basis for screening novel insecticidal candidates docx

... sequencing.Acknowledgements The authors wish to thank K D. Spindler for helpfuldiscussions during the course of the work, and the members of each of the laboratories whose technicalassistance contributed to the effort. ... a stretch of 35–37 aminoacids that lie just to the N-terminal side of the DBD(Fig. 1C). The EcRA isoforms from the two speciesshare a few similar motifs in the middle region of the A ⁄ B domain ... andpotentiators.Utility of the cell culture as a screening assayfor novel agonists The differences in characteristics of the ecdysteroidreceptors from the two species studied here, and the general...

Tài liệu Báo cáo khoa học: Regulation of dCTP deaminase from Escherichia coli by nonallosteric dTTP binding to an inactive form of the enzyme ppt

... in the main chain of the subunit, or in the interaction of subunits within the trimer that linked the conformation of residues 120–125 to the position of the active site lip and closure of the ... rates) prior to and after the transition of the enzyme to amore active form, respectively, t is the time and s is the lag-time. The rate constant, k, for the activation of the enzyme isobtained ... movement of the lip formed bya helix 2 and b strand 5 [18]. Apparently, movement of the active site lip prevented binding of the C-terminalresidues over the active site, or the absence of the C-terminal...

Tài liệu Báo cáo khoa học: Structure of peptidase T from Salmonella typhimurium doc

... areshowninblueiftheybelongtothecentralb sheetofthecatalyticdomain,greeniftheybelong to the four-stranded bsheetofthedimerization domain, and ligh t blue if theybelong the smaller bsheets of the catalyticdomain. ... Thisregion of similarity contains two of the ®ve ligands thatcoordinate the two zinc ions in the active site of CG2, forwhich the three-dimensional structure is known [7]. Pepti-dase T h as therefore ... CG2, the lack of clear homology outside these regions, and the unique tripeptidase speci®city of peptidase T, suggested that the structure of the two enzymes would in part differ. Wereport the...

Báo cáo khoa học: Role of different moieties from the lipooligosaccharide molecule in biological activities of the Moraxella catarrhalis outer membrane pot

... moieties. The ions with m ⁄ z of 1519.6and 1541.6 are mono- and di-sodiated forms of the de-O-acylated LOS and the ion with m ⁄ z of 1479.6 is the anhydrous form of the de-O-acylated LOS. The ion ... de-O-acylated LOSs from the wild-type O35Eand the mutant O35Elgt3 were further analyzed byMALDI-TOF MS (Fig. 4). The mass spectral data of de-O-acylated LOS from O35E was consistent with the reported structure ... respectively. The low molecular mass with m ⁄ z of 895.4 is from the Y-type fragment ion from the de-O-acylated lipid-Aportion (as observed in the negative mode). The ions with m ⁄ z of 917.4 and 877.4 (unmarked)...

Báo cáo khoa học: Characterization of a cathepsin L-associated protein in Artemia and its relationship to the FAS-I family of cell adhesion proteins pot

... pI values of the major bands in e and f, while the numbers at the left are the pI values of standard proteins. The arrow at the right represents the pI value of 6.84 calculated for the unmodiﬁedCLAP ... pI6.0werecommontoeachofthemajorCLAPfractions,although this does not mean that these are identicalisoforms. Overall, Artemia CLAP appears to contain fourisoforms in nearly equal a mounts, but these isoforms ... Liu11Department of Biological Sciences, University of Windsor, Windsor, Ontario, Canada;2Department of Molecular Cell Biology,Sylvius Laboratory, Leiden, the NetherlandsWe reported previously that the...

Báo cáo khoa học: Evaluation of potential regulatory elements identi®ed as DNase I hypersensitive sites in the CFTR gene doc

... for the CFTR gene. The aim of t his work w as toevaluate the regions of th e CFTR gene containing the DHSto identify those containing i mportant regulatory elements.In vitro analyses of the ... show a proportionate increase in the intensity of the DHS fragments in the control samples. In Fig. 7A the forskolin -treated chromatin shows a relative increase in the intensity of the DHS10a ... ).Activation of CFTRexpressionChromatin was extracted from untreated Caco2 andCapan1 cells or after incubation with forskolin or mitomy-cinCandthendigestedwithDNaseI.Toensurethatcontrol and drug-treated...

Báo cáo khoa học: Characterization of isocitrate dehydrogenase from the green sulfur bacterium Chlorobium limicola A carbon dioxide-ﬁxing enzyme in the reductive tricarboxylic acid cycle ppt

... been reported. Furthermor e, the catalyticproperties of IDH for the redu ctive carboxylation are muchless studied in comparison with those f or the o xidativereaction.In this report, we isolated ... buffers with pH values from 8.0 to 9.0, and Chesbuffers with pH values f rom 8 .7 to 10.0 were used for the assay.RESULTSIsolation of the idhgene from C. limicolaIn the cell-free extract of C. ... the catalytic properties of NADP-dependent IDH f romS. cerevisiae (Sc-IDH) in order to compare the properties of monomeric IDHs with those of a dimeric enzyme. The optimum pH for the decarboxylation...

Báo cáo khoa học: Isolation of a putative peroxidase, a target for factors controlling foot-formation in the coelenterate hydra docx

... structure of the clones, Dr Irm Hermans-Borgmeyer for critical reading of the manuscript, and Oliver Sperl andSimon Hempel for help with the ﬁgures. This work was supported part of the time by the ... for the generation of the 3¢ and 5¢ ends by performing PCRs either with the 3¢ RACE system (GibcoBRL) or with DNA of a(ZAP cDNA library of H. vulgaris as template. For libraryconstruction the ... four or ﬁve washes of the gel inH2O. The arrows indicate the stained bands in the gel. I is the majorperoxidase activity of hydra residing in the foot of the animal, IIindicates another...

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