Báo cáo y học: "A novel human ex vivo model for the analysis of molecular events during lung cancer chemotherapy" pptx

... of the patients. The purpose of the present study was the development of a human ex vivo tissue culture model and the analysis of the effects of conventional chemotherapy, which then can serve ... the individual trend of response.BioMed CentralPage 1 of 11(page number not for citation purposes)Respiratory ResearchOpen AccessResearchA novel human ex vivo model for the analysis of ... (8/20SCC) of all tested specimens, however, there were norelated patterns of the drug-induced effects.Discussion The major focus of this study was to examine the reliabil-ity of a novel ex vivo...

Báo cáo y học: "A novel method to identify and characterise peptide mimotopes of heat shock protein 70-associated antigens" pptx

... removed for analysis of IFN-γ production by ELISAafter 2 days. These cells were then cultured for a further 10days in the presence of IL-2, following which they were re-stimulated either with the ... activation of lym-phocytes must be dependent upon the uptake of the pep-tide by iDCs and its representation to lymphocytes and(b) it is not the result of any adjuvant-like contaminantpresent in the ... to the design and supervi-sion of the immuno-stimulatory assays. TCJ and UB con-ceived and designed the methods for the isolation of the peptide mimics of Hsp-PCs and identified all of the...

Tài liệu Báo cáo Y học: A novel, inducible, citral lyase puriﬁed from spores of Penicillium digitatum docx

... monomeric size of these hydratase/aldolase enzymes. All of these enzymesexhibit more then 75% of their maximum activity at pH 7.6, the optimum pH of citral lyase [27,30–32]. Whereas, manybacterial ... change during induction.Stability of citral lyase activity The activity and stability of citral lyase was dramaticallyaffected by the addition of 20% (v/v) glycerol and 1 mMEDTA. When glycerol ... standard.RESULTSInduction of citral lyaseInitially, the reproducibility of the results was hindered byvariations in the citral lyase activity of the P. digitatumspores. Remarkably, lyase activity was found to be...

Báo cáo y học: "A mouse embryonic stem cell bank for inducible overexpression of human chromosome 21 genes." ppsx

... wereretained for further analysis. Additional file 22 shows the raw data of the proteomic analysis by 2DGE following the overexpression of Runx1. The detailed spot quantifica-tion data, in the form of ... cellsand the non-induced state (in the cases of Bach1 andGabpa, for example) was not statistically significant andtherefore can be explained by the biological variability of expression levels of these ... (PromegaItalia, Milano, Italy). YFP fluorescence assay to detect the expression of the YFP reporter was performed using the DM6000 Leica Microscope.Karyotyping The analysis was performed on 20 different...

Báo cáo Y học: A single charged surface residue modiﬁes the activity of ikitoxin, a beta-type Na+ channel toxin from Parabuthus transvaalicus doc

... modeling The SWISS-PDB VIEWERsoftware from the EXPASYserver(http://www.expasy.ch) was used to visualize and compare the effect of the substitution of a glutamic acid for a glycineon the structure ... Hammock11Department of Entomology and Cancer Research Center,2Section of Neurobiology, Physiology and Behavior, and3Department of Chemistry and Superfund Analytical Laboratory, University of California, ... accomplished as described previ-ously for birtoxin [6]. Brie y, the cysteine residues of the peptide were reduced and carboxymethylated by incubatingin 6Mguanidine hydrochloride, 0.1MTris/HCl (pH...

Báo cáo y học: " A QTL on mouse chromosome 12 for the genetic variance in free-running circadian period between inbred strains of mice" potx

... for the analysis and display of circadian activity data. Activity Profile plots the average activity for specified dates as a function of cir-cadian time. We grouped activity events into thirty ... calculated the mean activity during the last eightdays under LD 12:12. We assessed the phase of minimumactivity during the dark part of the cycle.Calculating circadian period of locomotor activityAfter ... timing system: searching for a physiologicalrole for aryl hydrocarbon receptor. Toxicol Sci 2007,95:172-181.47. Poland A, Palen D, Glover E: Analysis of the four alleles of the murine aryl hydrocarbon...

Báo cáo hóa học: " A rapid, convenient, solventless green approach for the synthesis of oximes using grindstone chemistry" docx

... azaheterocycles [8] are some of the synthetic applications of oximes. They are also useful for selective a-activat ion [9] and are extensively used asintermediates for the preparation of amides by the ... industry deals with larger quantity of materials, these factors are particularly very importanttherein. Over the years, many reagents and catalystshave been developed for the synthe sis of oximes. ... Mizuno M, Miyake K, Minami N (1996) Synthesis of (2R)-1-(4-Chloro-2-pyridyl)-2-(2-pyridyl)ethylamine: a selective oximereduction and crystallization-induced asymmetric transformation. Synthesis8:991–9968....

Báo cáo y học: "Monoclonal Antibodies against Nucleophosmin Mutants: Potentials for the Detection of Acute Myeloid Leukemia" ppt

... Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Department of Laboratory Medicine, Chong-qing Medical University, Chongqing 400016, China 2. Center for Hematology, ... [3, 4]. The most common molecular variant of the NPM1 gene is mutation A, accounting for about 75-85% of cases. It is due to a duplication of TCTG tetranucleotide at the C-terminus of the NPM1 ... given 2 days before harvesting the spleen cells. Experiments with injected mice were performed under the guidelines for care and use of experimental animals. 2.5 Cellular fusions When the anti-NPM-mA...

Báo cáo y học: "A novel hybrid aspirin-NO-releasing compound inhibits TNFalpha release from LPS-activated human monocytes and macrophages" docx

... Itis, therefore, possible that changes such as these to recep-tor expression, signalling pathways and to the biosynthe-sis of cytokines, which normally occur during the maturation of monocytes ... (acetylsalicylic acid) was first synthesized in 1899and was the first example of the family of nonsteroidalanti-inflammatory drugs (NSAIDs). Its therapeutic usesinclude the treatment of headache, ... effectiveprophylactic against thrombotic events in the cardiovas-cular system. The anti-inflammatory effects of aspirin areachieved primarily through inhibition of cyclooxygenase-mediated synthesis of...

Báo cáo y học: " A novel small molecule target in human airway smooth muscle for potential treatment of obstructive lung diseases: a staged highthroughput biophysical screening" pps

... K, Xu N, Zhen Y, Boniface JJ: Anintegrated strategy for the discovery of drug targets by the analysis of protein-protein interactions. International Journal of Mass Spectrometry2004, 238:119-130.doi:10.1186/1465-9921-12-8Cite ... 0.5%w/v cyclodextrin as a vehicle for the delivery of smallmolecules.Among the seven scaffolds which showed activity in the FP assay as small molecule analogs of pH SP20, onlya small subset of compounds ... twisting cytometry (MTC) and changes in celltraction force using Fourier transform traction micro-scopy (FTTM). Finally, scaling up to the level of anintact tissue, we validated the potency of the...

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