... USA) (150 ngÆ well )1 ) and phRL-CMV,containing Renilla luciferase cDNA (5 ngÆwell )1 ), usingLipofectamine and OptiMEM medium (Invitrogen, Carls-bad, CA, USA). In pNF-jB-Luc the expression of ... Regardless of dopamine treatment, overexpres-sion of a-synuclein significantly affected the levels of four proteins [stathmin 1, glutathione S-transferase(GST)p, Ran 1 binding protein and C1q binding ... promotercontaining five NF-jB binding sites. After 7 h of incuba-tion, the transfection mixture was replaced with completeDMEM containing, or not, 0.250 mm dopamine, in thepresence of 700 UÆmL )1 catalase....