... place after binding to proteins such aslipopolysaccharide-binding protein (LBP) and CD14[8,9], which finally transport the compounds to integralmembrane proteins such as Toll-like receptor 4(TLR4) ... containing 2 mml-glutamine,100 UÆmL)1penicillin, and 100 lgÆmL)1streptomycin, added to the wells of a 96 well dish, and precipitated by addition of polyfect (1 lLin20lL serum-free DMEM) and incubationfor ... ngÆmL)1macrophagecolony-stimulating factor (M-CSF)14 in RPMI 1640 medium(endotoxin < 0.01 endotoxin units (EU)ÆmL)1 in Limulustest; Biochrom, Berlin, Germany) containing 2 mml-gluta-mine, 100 UÆmL)1penicillin, and...