Báo cáo khoa học: "Development of real time PCR for detection and quantitation of Dengue Viruses" potx

... primer sets for detection of all four serotypes of DENV [11]. In thepresent study, we describe the development of a DENV-specific TaqMan based real- time PCR for detection and quantitation of all ... 123(5):615-628.BioMed CentralPage 1 of 8(page number not for citation purposes)Virology JournalOpen AccessMethodologyDevelopment of real time PCR for detection and quantitation of Dengue VirusesKR Gurukumar, ... The detection limit of the assay varied for the four serotypes of DENV, the sen-sitivity of detection was highest for DENV-2 and lowest for DENV-4 (Fig. Fig. 2C–F, Table 3). Detection and Quantitation...

Báo cáo khoa học: "A multiplex real-time PCR for differential detection and quantification of Salmonella spp., Salmonella enterica serovar Typhimurium and Enteritidis in meats" pps

... al. Detection limit and standard curve of the multiplex real- time PCR The detection limit and standard curve of the multiplex real- time PCR was determined using S. Typhimurium ATCC 14028 and S. Enteritidis ... previous real- time PCR assays [8,27]. These advantages improved the multiplex real- time PCR assay in terms of shortening turnaround time for bacterial detection and reducing the risk of cross- ... recent times, real- time PCR assays have been successfully applied in the detection of bacterial pathogens in food products [11,12,24,25]. A single real- time PCR assay was applied for specific detection...

Báo cáo hóa học: " Research Article Real-Time Cardiac Arrhythmia Detection Using WOLA Filterbank Analysis of EGM Signals" pot

... merged to obtain wideband-subband sig-nals to be used for wideband event detection. The results of the wideband and narrowband detections are then combined for robust detection. As the intention ... of the event detection algorithm. of the subband signals have baseband spectrums, to combinesubbands one has to modulate the bands to line them up se-quentially. For merging two subbands, for ... 1, , K − 1, wherem is the subband time index. For real input signals, onlyhalf of the subbands are stored and processed due to Hermi-tian symmetry. The subband time- index m is updated everyR=...

báo cáo khoa học: " Development-dependent changes in the tight DNA-protein complexes of barley on chromosome and gene level" pot

... 150ng of the first strand cDNA were used as a template for PCR amplification performed in 30 μl of the reaction mix-ture with a final concentration of 1× PCR buffer (Fermen-tas), 3 mM of MgCl2, ... leaves and coleoptiles at two stages of development (Zadoks 07 and 10). In dry seeds most markers of both chromosomeswere found in F and R1 fractions. Only four and five mark-ers of 1H and 7H ... 5 times, and aliquots of the R1 and R2fractions were diluted twice. 5 μl of the diluted solutionwas taken for the amplification reaction. The final PCR reaction mix contained: about 100 ng of...

Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): reactivity and structure of metal–thiolate clusters* doc

... is a soft ligand and therefore shows a preference for soft metals. More-over the structures of metallothioneins are not rigid and hence there is little selectivity concerning the size of the ... approach of thetwo domains. Also, the additional binding of M(II)induced slow, but appreciable, non-covalent dimeriza-tion of MT3 [40% for Zn(II) and 80% for Cd(II)].Subsequent analysis of 113Cd(II) ... cluster for Zn(II) and Cd(II) [18]. Initially,information about the presence of two separate metal–thiolate clusters came from spectroscopic studies and comparison with other MTs of Zn(II)- and...

Tài liệu Báo cáo khoa học: Poly(silicate)-metabolizing silicatein in siliceous spicules and silicasomes of demosponges comprises dual enzymatic activities (silica polymerase and silica esterase) doc

... rational application of silicatein inthe fabrication of (new) biomaterials based on layeredsilica, of titania and of zirconia [32]. This view is basedon the ﬁnding of a dual role for silicatein ... (Asn) and serine (Ser) [4,12]. In the active center of silicatein, thehydroxyl group of Ser26 and the imidazole of His165(catalytic diad) have been shown to play key roles inthe condensation of ... displacement of p-aminophenol and formation of a (alkoxyl)-monosilane. This reac-tion is facilitated by hydrogen bonding between the imidazole nitro-gen of the conserved histidine (His) and the...

Tài liệu Báo cáo khoa học: Structural insights into the substrate speciﬁcity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt

... understanding of the genericprinciple of catalysis for this family of proteases.Results and DiscussionFold of ervatamin-AThe overall structure and fold of ervatamin-A is simi-lar to that of ... range of substrateconcentrations and the time of incubation were standardized for each substrate. An extinction coefﬁcient of 8800 at410 nm for pNA was used for the calculations. The softwaregraphpad ... compriseddenaturation at 95 °C for 60 s, annealing at 60 °C for 90 s, and extension at 72 °C for 90 s) and a ﬁnal extension stepat 72 °C for 15 min. The forward primers for PCR weredesigned according...

Tài liệu Báo cáo khoa học: The role in the substrate speciﬁcity and catalysis of residues forming the substrate aglycone-binding site of a b-glycosidase docx

... units, in spite of its favorable interac-tion with alkyl moieties. Therefore, the replacement of E194 and K201 may be an important mechanism for changing the specificity of Sfbgly for aglycone.The ... details about the role of different resi-dues of the aglycone-binding site in the stabilization of ESà and the interdependence between the binding of aglycone and the positioning of glycone in subsite ... Behaviour and Analy-sis of Rapid Equilibrium and Steady-State EnzymeSystems. John Wiley and Sons, New York, NY.Molecular basis for specificity of a b-glycosidase L. M. F. Mendonça and S. R....

Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx

... curves. Squares and dotted line: data and fittingwith PHI/PHO ratio of 0.5. Triangles and continuous line: data and fitting with PHI/PHO ratio of 1. Asterisks and broken line: data and fitting with ... (University of Turin) for mass spectroscopy analysis, to A. Conti and G. Giuffrida (CNR-Torino) for N-terminal sequence determination and to D. Cavazzini(University of Parma) for helpful discussion and ... firsthypothesis and therefore a direct involvement of PHI inelectron transfer. This conclusion is consistent with the verylow degree of identity between the N-terminal sequence of PHI and those of ferredoxin-like...

Báo cáo khoa học: Protein transport in organelles: The composition, function and regulation of the Tic complex in chloroplast protein import pptx

... channelfunction (e.g. in ligand-gated and voltage-gated K+channels) [16], and thus could provide an explanation for the observed channel activity of Tic110, as well as for the binding of transit peptides ... of the other isoform. However, analysis of double knockouts of both Hsp93 homologues didnot result in the identification of double homozygotes,establishing that Hsp93 function is essential for ... theimport of preproteins. A prominent and difficult task for future studies will therefore be to determine howorganelle metabolism and physiology influences proteinimport by the Tic complex and by...

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