... possible the study of the HCV replication cycle, in vitro. Studies utilizing the HCVpp and
HCVcc systems have increased our insight into the early steps of the viral replication cycle of HCV,
such ... the HCV
replication cycle was hampered due to difficulties in growing and propagating the virus in an in vitro
setting. The advent of the HCV pseudo parti...
... absence of tetracycline to induce specific protein expressionFigure 3
Different features of apoptosis induced by the HCV-core protein in UC cells cultured in the presence or
absence of tetracycline ... investigate whether the HCV core protein
exerts an enhancing effect on the activation of the death
Different features of apoptosis induced by the HCV-core protein in UC cells...
... expression constructs
NS4B FL
Forward primer, GTGGGTACCATGTCACACCTCCCTTACATCGAACAG
Reverse primer, TAGTCTAGAGAGCCGGAGCATGGCGTGGAGCAGTC
NS4B-CTD
Forward primer, GTGGGTACCATGGCGATACTGCGTCGGCACGTGGGC
Reverse ... This indicates that membrane
association is not affected by the suggested RNA binding
characteristics of the domain in the presence of RNA.
Clearly, the HCV life cycle is achie...
... over-looking the
economic significance of their ill health, assuming they progress to cirrhotic HCV or develop
hepatocelluar carcinoma due to HCV chronicity.
Background
Hepatitis C virus (HCV) infection ... also increases the rate of HCV replication.
Plasma levels of pro inflammatory cytokines like tumor
necrosis factor- alpha are increased in acute alcoholic hep-
atitis and suc...
... expression constructs
NS4B FL
Forward primer, GTGGGTACCATGTCACACCTCCCTTACATCGAACAG
Reverse primer, TAGTCTAGAGAGCCGGAGCATGGCGTGGAGCAGTC
NS4B-CTD
Forward primer, GTGGGTACCATGGCGATACTGCGTCGGCACGTGGGC
Reverse ... FL
NS4B-deltaCTD
Forward primer, as NS4B FL
Reverse primer, TAGTCTAGAGACCGACGCAGTATCGCTGCGCACACGAC
NS4B-CTD sub-Cys
Forward primer, as for NS4B-CTD
Reverse primer, AGATCTAGAGAGCCGGAGGATG...
... over-looking the
economic significance of their ill health, assuming they progress to cirrhotic HCV or develop
hepatocelluar carcinoma due to HCV chronicity.
Background
Hepatitis C virus (HCV) infection ... pro inflammatory cytokines like tumor
necrosis factor- alpha are increased in acute alcoholic hep-
atitis and such cytokines induce insulin resistance and
glucose intolerance whi...
... virus, in and
of itself, can directly induce cytoplasmic lipid
accumulation. Further studies examining the genotype 3
virus are warranted to further recognize the process
involved in HCV-induced ... [26] The core protein has been located
at the surface of lipid droplets within the cytoplasm in cell
cultures that are transfected with HCV while absent in
control cells...
... used in molecular epidemiology
studies, where exact subtyping is needed. In clinical
practice, HCV genotype can be determined by various
commercial kits, using direct sequence analysis of the ... mis-subtyping may occur in 10 to 25% of
cases, related to the studied region (5’ noncoding region)
rather than the technique used. These errors have no
clinical consequences, becaus...