Báo cáo khoa học: " Characterization of untranslated regions of the salmonid alphavirus 3 (SAV3) genome and construction of a SAV3 based replicon" ppsx

Báo cáo khoa học: Methylcitrate synthase from Aspergillus fumigatus Propionyl-CoA affects polyketide synthesis, growth and morphology of conidia ppt

... 5¢ 3 cDNAmcsAAf_up GAC CAT CCC TTG ATA GCA TCcDNAmcsAAf_down GAT ATC ACA GGC TCA CAG GNotMcsAAf_up CCG CCT TCA GAG CGG TCT TGNotMcsAAf_down CCG CCT CCG GAG TCC TCT TCAfumMcsAup GGC CTG AGG ... dehy-drogenase from rabbit muscle (Roche Diagnostics, Mann-heim, Germany) as described in [3] .Determination of acyl-CoA synthetase activity and preparation of intracellular acyl-CoAAcetyl-CoA and ... (2005) 36 15 36 30 ª 2005 FEBS 36 23 Activation of acetate and propionate to the corresponding CoA-estersMethylcitrate synthase and PDH complex from A. nidulans and A. fumigatus display very similar...

báo cáo khoa học: "Parthenocarpic potential in Capsicum annuum L. is enhanced by carpelloid structures and controlled by a single recessive gene" docx

... aberrant form of Ara-bidopsis ARF8 also conferred parthenocarpy in Arabi-dopsis and tomato, indicating ARF8 as an importantregulator in the control of fruit set [4]. Mapping of a parthenocarpic QTL ... 7). Line 3 was used as a parthenocarpic parent (Pp) and Lamuyo B, ORF2#1, and Parco as non-parthenocarpic parents (Pn). F2progenieswere obtained for all three crosses, and also a backcr osswith ... study the genetic basis and inheritance of the parthe-nocarpic potential in C. annuum,theparthenocarpicgenotype Line 3 was crossed with the non-parthenocar-pic parents Lamuyo B, OR F2#1 (a male...

Báo cáo khoa học: " Characterization of untranslated regions of the salmonid alphavirus 3 (SAV3) genome and construction of a SAV3 based replicon" ppsx

... inCloning and characterization of SAV3 5'- and 3& apos;-endsFigure 1Cloning and characterization of SAV3 5'- and 3& apos;-ends. The 5'- and 3& apos;-ends of SAV3 were cloned and sequenced ... citation purposes)Virology JournalOpen AccessShort report Characterization of untranslated regions of the salmonid alphavirus 3 (SAV3) genome and construction of a SAV3 based repliconMarius ... (SAV1-6). Here we report the cloning and characterization of the 5'- and 3& apos;- untranslated regions (UTR) of a SAV3 isolated from Atlantic salmon in Norway. The sequences of the UTRs are...

Báo cáo khoa học: Characterization of the 5¢ untranslated region of a and b isoforms of the human thromboxane A2 receptor (TP) Differential promoter utilization by the TP isoforms doc

... generate the recombinantplasmids pGL3b:Prm1; pGL3b:Prm2 and pGL3b:Prm3,each in pGL3Basic and pGL3e:Prm1; pGL3e:Prm2 and pGL3e:Prm3, each in pGL3Enhancer. The ﬁdelity of allrecombinant plasmids ... TXA2synthase, acts as a potent agonist of plateletactivation and aggregation and mediates a diversity of actions in a number of other target cell or tissue types [1].TXA2signals through interaction ... transcripts and determination of their transcriptioninitiation sites The organization and the exon-intron boundaries of the human thromboxane (TX) A 2receptor (TP) gene and the theoretical range of...

Tài liệu Báo cáo khoa học: Characterization of 1H NMR detectable mobile lipids in cells from human adenocarcinomas doc

... from human adenocarcinomasAnna Maria Luciani1, Sveva Grande1, Alessandra Palma1, Antonella Rosi1, Claudio Giovannini2,Orazio Sapora 3 , Vincenza Viti1 and Laura Guidoni11 Dipartimento ... irradiated: black) and A ⁄ (Lys + Ala) (controls: dotted white;irradiated: dotted black) as obtained from 1D and 2D COSY spectra of HeLa and MCF-7cell samples. Data are the mean ± SDvalues of ... spectralﬁtting of samples without ML signals. The presence of these peaks was paralleled by the existence of lipidcross peaks in the 2D spectra (Table 1 and Fig. 3B). The cross peak A, due to the interaction...

Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx

... with the followingprimers: QsCgClp1 (5¢-CTTCCTCCGCTTCCATGA -3 ) and QaCgClp1 (5¢-CCATGAAGTCCGCGAATC -3 ); and QsCgClp2 (5¢-GCATAGCGATGTGGACGA -3 ) and QaCgClp2 (5¢-GAGGACCGAGACCGTGAA -3 ). The abbreviations ... Mohanty AK, Singh G, Paramasivam M, Saravanan K,Jabeen T, Sharma S, Yadav S, Kaur P, Kumar P, Srini-vasan A et al.(20 03) Crystal structure of a novel regula-tory 40-kDa mammary gland protein ... Kobori K, Miyashita K, Fujii T, Sakai H,Uchida M & Tanaka H (19 93) Identiﬁcation of glutamicacid 204 and aspartic acid 200 in chitinase A1 of Bacil-lus circulans WL-12 as essential residues...

Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt

... cells and a primer set of the forward pri-mer DN-5R and the reverse primer DN+854X (sequences5¢-CGGAATTCTCAGGATGAGGGGCATGAAG -3 and 5¢-CGCTCGAGGCTGCTCACTTCAGCATCAC -3 , res-pectively) and directionally ... Kominato Y, Yamamoto F & Takizawa H(2002) Characterization of the human ABO gene pro-moter in erythroid cell lineage. Vox Sang 82, 39 –46. 34 Yasuda T, Awazu S, Sato W, Iida R, Tanaka Y &Kishi ... DN)243R or DN)183R, the sequences of whichwere 5¢-CGGAATTCACATTTGCCCCAGGGAAGGTC- 3 and 5¢-CGGAATTCTCCTGCCCAGGACCCGAGG -3 ,respectively. The DNASE1 expression plasmids ex-pDN1 and ex-pDN 1a, containing...

Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc

... FEBS Characterization of the interaction between the plasmamembrane H+-ATPase of Arabidopsis thaliana and a novelinteractor (PPI1)Corrado Viotti, Laura Luoni, Piero Morandini and Maria Ida De MichelisDipartimento ... of the ﬁrst 88amino acids of PPI1 may participate in the interactionwith the H+-ATPase and makes PPI1588His6 a suitabletool to study the mechanism of action of PPI1. The analysis of the ... binding of 14 -3- 3s.Results The C-terminus of isoform 1 of the PM H+-ATPase of A. thaliana (AHA1) interacts with the ﬁrst 88 aminoacids of PPI1 [22], indicating that the PM H+-ATPasebinding...

Tài liệu Báo cáo khoa học: Characterization of a recombinantly expressed proteinase K-like enzyme from a psychrotrophic Serratia sp. ppt

... 5¢-TTGATCGATTCTGTCTATGCCCCA -3 along with the adaptor primers: AP1 (5¢-GTAATACGACTCACTATAGGGC -3 ) and AP2 (5¢-ACTATAGGGCACGCGTGGT -3 ).Nested PCR was carried out in a ﬁnal volume of 50 lLcontaining 1 lL of a genome ... 5¢-GATGAAAATCCTAACCTCTCCCCCGCACAG- 3 ; OP7, 5¢-ACTGCACCTACGGCGGGTCGTTGGTACGTG -3 ; NP4, 5¢-GACACCGTAGGTTGAGCCGCCAATCGTCCC -3 ; NP5, 5¢-CTTTAACTTGTTGGGCACTGGCATTG -3 ; NP6, 5¢-TTGATCGATTCTGTCTATGCCCCA -3 ... PCR was performed in 50 lL containing 1 ng of genomic DNA as template, 0.2 mm dATP, dCTP, dGTP and dTTP, 0.2 lm of upstream primer (OP17: 5¢-GAAAAACCATGGTGAATGAATACCAAGCGACT -3 ) and downstream...

Tài liệu Báo cáo khóa học: Characterization of the products of the genes SNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiae pptx

... 5¢-ATACCATGGACAAAACCCACAGTACAATGP1OH 5¢-CATATGCACAAAACCCACAGTACP2O 5¢-TATGGATCCTTAATTAGAAACAAACTGTCTGATAAACP2OH 5¢-CTCGAGATTAGAAACAAACTGTCTGATAAACCP1Z 5¢-ATACCATGGCTGGAGAAGACTTTAAGATCP1ZH ... min, the absorbance of the supernatant was read at 36 3 nm. The absorbance of the reduced form of APAD (APADH) was linear over the 2–100 lMrange of glutamate with a molar extinctioncoefﬁcient of ... 5¢-CATATGACTGGAGACTTTAAGATCP2Z 5¢-TATGGATCCTCACCACCCAATTTCGGAAAGP2ZH 5¢-CTCGAGCCACCCAATTTCGGAAAGTTable 2. Over-expression plasmids for Sno1p and Snz1p prepared in thisstudy. Symbols O, Z and...

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