Báo cáo y học: "Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b protein in-vitro and in-vivo" pptx
... characterization of the natural genomic deletion in
SARS-Coronavirus strain Frankfurt-1 open reading frame 7b
reveals an attenuating function of the 7b protein in- vitro and in- vivo
Susanne Pfefferle
1
, ... SARS-CoV accessory gene products have been
shown to be involved in the induction of apoptosis,
including the 7a and 7b protein...
... Journal
Open Access
Research
Reverse genetic characterization of the natural genomic deletion in
SARS-Coronavirus strain Frankfurt-1 open reading frame 7b
reveals an attenuating function of the 7b ... SARS-CoV accessory gene products have been
shown to be involved in the induction of apoptosis,
including the 7a and 7b proteins [18,46,4...
... NICN1,wehavenow
analyzed the human and murine orthologs and provide
initial data on the expression profiles and cellular localiza-
tion of the protein product.
MATERIALS AND METHODS
General methods
Standard ... of the cDNAs in the used
panels is normalized to four different housekeeping genes.
The PCR analyses of the panels was carried out according
to the man...
... participated in the design of the study and analysis of the
data. FC participated in the design of the study and in the anal-
ysis of data and helped to draft the manuscript. PM cloned and
sequenced ... revision of the study. AS partic-
ipated in the analysis and interpretation of data and helped to
draft the manuscript. RR participated in...
... FcγRIIb staining was observed in the synovial lin-
ing and sub-lining layers, perivasculary and inside lymphocyte infiltrates
(e). (d) No staining of the RA synovium was observed when the anti-
FcγRIIb ... very similar and both receptors were
often found perivasculary and always in the synovial lining and
sub-lining layers of the synovium. FcγRII and III positiv...
... information, the fraction of total
RNA that delivers coding information to the protein-
synthesizing machinery (i.e. mRNA ) is in trinsically labile
and continuously synthesized. The steady-state level of
mRNA ... vary and is a
function of RNA synthesis and decay, the control of
mRNA stability plays an essential role in the regulation
of gene expression. As...
... protein as judged by SDS/
PAGE. The activity of the pure recombinant protein was
80% of that of t he wild-type protein in both the lysozyme
and chitinase assays. Attempts to further purify the
recombinant ... sp. [16]
and B. circulans [33] chitinases, where the Asp fi Asn
mutants retained only 0.03% and 0.2% of the wild-type
activity, respectively. This sugges...
... cysteine residues:
the 2Cys-Prx subgroup, and 1Cys-Prx subgroup contain
two and one conserved cysteines, respectively. 2Cys-Prx
proteins are reduced by the AhpF protein in bacteria, and
by the ... proteins in databases. Tryptic peptides were puri®ed by RP-HPLC and some of them were to tally or
partially analyzed by Edman sequencing (ááá) and/ or by MALDI-TOF mass spectr...
... with
MEGALIGN
program), and 65% are conserved using
BLAST
analysis
comparing all of the vitamin K-dependent carboxylases.
In contrast, the C-terminal 25% of the protein shows no
homology to any of the other vitamin ... Using the bovine
numbering system (Fig. 1), we observe Arg179 rather than
histidine, Thr430 rather than alanine, Pro654 rather than
serine, Met726 rather...
... contraction
by activating ryanodine receptors of the sarcoplasmic
Fig. 1. Isolation of tigrin from the Duvernoy’s glands of R. tigrinus
tigrinus. (A) The extract from Duvernoy’s glands of R. tigrinus ... [51,52]. Antigen 5 proteins are
components of the venoms of wasps and ants, and they are
thought to be involved in the provocation of an acute and
localized...