báo cáo khoa học: " Characterization and isolation of a T-DNA tagged banana promoter active during in vitro culture and low temperature stress" ppt

... article Characterization and isolation of a T-DNA tagged banana promoter active during in vitro culture and low temperature stressEfrén Santos†1,2, Serge Remy†1, Els Thiry1, Saskia Windelinckx1, ... citation purposes)African plantain-type banana, is also functional in thecommercial dessert banana 'Grand Naine'. These resultsare encouraging in view of generating cisgenic plants. ... Belgium) and commerciallysequenced. Sequences were analyzed with BLASTn and BLASTx http://www.ncbi.nlm.nih.gov/BLAST/ programsagainst the GenBank database, and against a banana ESTdatabase donated...

Báo cáo khoa học: Activator-binding domains of the SWI ⁄ SNF chromatin remodeling complex characterized in vitro are required for its recruitment to promoters in vivo pot

... Tra1 as a direct target of Gal4, and several other acidic activators[31]. Thus, the FRET and cross-linking approachesappear to cross-validate each other. A photo-cross-linking approach has ... nucleosomes. Taken together,these ﬁndings suggest that the predominant coactivatingrole of SWI ⁄ SNF may be at steps downstream of activa-tor binding rather than as a facilitator of activator bind-ing, ... 2009)doi:10.1111/j.1742-4658.2009.06979.xInteraction between acidic activation domains and the activator-bindingdomains of Swi1 and Snf5 of the yeast SWI ⁄ SNF chromatin remodelingcomplex has previously been characterized in vitro. ...

Báo cáo khoa học: Three-dimensional structure of a thermostable native cellobiohydrolase, CBH IB, and molecular characterization of the cel7 gene from the ﬁlamentous fungus, Talaromyces emersonii ppt

... replaced by an alanine (Ala374), forming a more opentunnel; however, electron density in this area of 1Q9H ispoor. In 1GPI, neither asparagine residues are present and a histidine a nd an alanine ... GenBank AAA19802), and Tricho-derma reesei (T.reesei; GenBank CAA49596). Residues in white against a black background are amino acids that are identical or have a conservedsubstitution in all ... 5¢-RACEprimer CATGCGGTAAGGGTTGAAGTCACA-3¢;inner 5¢-RACE primer 5¢-GTTTGCTTCCCAGACATCCATC-3¢; outer 3¢-RACE primer 5¢-ATGCTGTGGTTGGATTCCGACTAC-3¢; and inner 3¢-R ACE primer 5¢-AACTCCTACGTGACCTACTCGAAC-3¢....

Báo cáo khoa học: The fabp4 gene of zebraﬁsh (Danio rerio) ) genomic homology with the mammalian FABP4 and divergence from the zebraﬁsh fabp3 in developmental expression pot

... specificity and affinity of FABP3 and FABP4, the transcripts and proteins of the two paralogous genes exist in multipletissues and are colocalized in several mammalian tis-sues, including mammary gland ... analysisPhylogenetic analysis of zebrafish fabp4 and fabp3 and other fish and mammalian FABP genes was performedusing clustalx [43]. The Antarctic fish H6-FABP and H8-FABP sequences were included in this analysis, ... (Gog) FABP4 (H6-FABP, AAC60354) and FABP3 (H8-FABP,AAC60359); N. coriiceps (Nc) FABP4 (H6-FABP, AAC60352) and FABP3 (H8-FABP, AAC60358); Parachaenichthys charcoti (Pc) FABP4 (H6-FABP, AAC60355);...

Báo cáo khoa học: Dissociation/association properties of a dodecameric cyclomaltodextrinase Effects of pH and salt concentration on the oligomeric state pot

... and was not involved in main-chain to main-chain hydrogen bonding with either the N- or the(b /a) 8-barrel domain. Interestingly, the C-terminaldomain was found to be critically involved in ... dissociation/association of CDase I-5Based on the information obtained about the 3D struc-ture of CDase I-5, the quaternary state of CDase I-5was likely to be maintained by the intrinsic capability of ... form a dodecamer and thatFig. 1. Apparent molecular mass of CDase I-5 at various pH valuesdetermined by analytical ultracentrifugation analysis.Dynamics of a CDase in the oligomeric state H S....

Báo cáo khoa học: On the mechanism of a-amylase Acarbose and cyclodextrin inhibition of barley amylase isozymes pdf

... a- amylasescontain a central catalytic (b /a) 8barrel domain (domain A) having an irregularly structured small domain B protrudingbetween b-strand 3 and a- helix 3 of the barrel, and a C-terminal, domain ... 13acting on a- 1,4 internal glycoside linkages in starch and related sugars [1]. a- Amylases occur widely in higher plants,animals, bacteria and fungi and are applied in severalimportant industries, ... This appears to be a general feature of the a- amylase family.Keywords: amylose; maltodextrin; acarbose; barley a- amy-lase; binding site. a- Amylase is a retaining glycoside hydrolase of family...

Báo cáo khoa học: Cleavage site analysis of a serralysin-like protease, PrtA, from an insect pathogen Photorhabdus luminescens and development of a highly sensitive and speciﬁc substrate pdf

... reactions as an internalstandard, because it was not hydrolysed by PrtA. Peakareas were normalized with the internal standard, and thedegree of cleavage was calculated from the reduction in ... specificity, and kinetic characterization of PrtAactivity on the new substrate.Results and DiscussionIdentification of PrtA cleavage sites in biologicalpeptidesTo obtain an initial view of the cleavage-site ... FX, McKay DB & Bode W (1995) Themetzincins – topological and sequential relationsbetween the astacins, adamalysins, serralysins, and matrixins (collagenases) define a superfamily of zinc-peptidases....

Báo cáo khoa học: The speciﬁcity of alcohol dehydrogenase with cis-retinoids Activity with 11-cis-retinol and localization in retina docx

... chain reaction and introduced restrictionsites (underlined) for BamHI at the 5¢ end (5¢-CTATCGGATCCATGAGCACAGCAGGAAAAG-3¢)andforEcoRI at the 3¢ end (5¢-CCACTTGAATTCTCAAAACGTCAGGACGGT-3¢). ... Gaur, V.P., Saari, J.C. & Milam, A. H. (1990)Immunolocalization of cellular retinol-, retinaldehyde- and retinoic acid-binding proteins in rat retina during pre- and post-natal development. ... CoommassieÒ Brilliant Blue (Sigma) stain technique.Protein concentration was determined by a dye bindingassay (Bio-Rad) using bovine serum albumin as standard[30].Enzyme kineticsStandard ADH...

báo cáo khoa học: "Long-term survival of a woman with well differentiated papillary mesothelioma of the peritoneum: a case report and review of the literature" docx

... pleural and peritonealsurfaces with ascites and pleural effusion was treatedsucc essfully with intraperitoneal, intrapl eural, and intra-venous carboplatin [12]. Our patient remained diseasefree ... at autopsy tohave extensive retroperitoneal, anterior abdominal wall,diaphragmatic, and pericardial invasion, culminating in a large embolism of tumor cells to the pulmonary artery[7]. A second ... generally con-sidered of low malignant potential and falls within a clini-cohistological spectrum of papillary peritoneal tumors in women ranging from mesothelial hyperplasia to papillarycarcinoma...

báo cáo khoa học: " Improving the delivery of care for patients with diabetes through understanding optimised team work and organisation in primary care" ppt

... and indi-vidual and organisational factors. A consistent finding in health services research is that thetransfer of research findings into practice is unpredictable and can be a slow and haphazard ... diabetes care, a combination of individual and team/organisational measures has greatpotential for added understanding.Theories on human behaviour (and especially adultbehaviour change) can be categorised ... Finland, 6Ottawa Health Research Institute, 1053 Carling Avenue, Room 2-017, Admin Building, Ottawa, ON K1Y 4E9, Canada and 7Department of Medicine, University of Ottawa, Ontario, Canada,...

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