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Báo cáo y học: " Quantitative PCR used to Assess HIV-1 Integration and 2-LTR Circle Formation in Human Macrophages" potx

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Báo cáo y học: " Quantitative PCR used to Assess HIV-1 Integration and 2-LTR Circle Formation in Human Macrophages" potx

... ferencesbetween integrated HIV-1 proviral DNA and uninte-grated HIV-1 cDNA in HIV-1 infected cells.Results HIV-1 Integration in U373 cells To verify Alu-gag two-step PCR could be used to detect HIV-1 integration ... and HIV-1 integration wasmeasured by two-step quantitative PCR, and (C) 2-LTR circle formation was measured by real-time PCR. (**p < 0.01)Figure 3 Quantitation of HIV-1 integration and 2-LTR ... antiretroviral integrase inhibitor, raltegravir, to distinguish between integrated and non-integrated HIV-1 cDNA in infected primary PBL, macrophages, and ahumanCD4+cell line. We detected HIV-1 integration by...
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Báo cáo y học: "Impaired vascular responses to parasympathetic nerve stimulation and muscarinic receptor activation in the submandibular gland in nonobese diabetic mice." ppt

... [28]. In autoimmune diseases, vascular cells can actively contribute to the inflammatory cytokine-dependent network in the bloodvessel wall. By interaction with invading cells, the activationmay ... were obtained using a cryostat (Leica Instru-ments, Nussloch, Germany) and were placed onto SuperFrostPlus glass slides (Menzel, Braunschweig, Germany). Haema-toxylin and eosin staining was ... cells contribute to atherosclerosis by cytokine- and innate-immunity-relatedinflammatory mechanisms. Innate Immun 2008, 14:63-87.29. Ross R: Atherosclerosis is an inflammatory disease. Am HeartJ...
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Báo cáo y học: "Validation of actigraphy to assess circadian organization and sleep quality in patients with advanced lung cancer" potx

... of healthyindividuals [11-15]. Care has been taken to fully specifythe instrumentation type, sampling mode and analysistools in order to allow inclusion of this study in thegrowing database ... line to a dataset when searching for a linear increasing or decreasingtrend and subsequently determining the probability thatthe s lope of the best-fitting line is different from zero.Using ... software(Ambulatory Monitoring, Inc). Rhythmometric analysis(using Chronolab v2) was done on these sleep/activitypatterns in order to assess d isruption and consolidationof sleep in lung cancer...
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Báo cáo y học: "Cigarette smoke regulates the expression of TLR4 and IL-8 production by human macrophages" pdf

... cellular lysateaccording to the methods of Tietze [31] with slight modi-fication [32]. Briefly, washed cells were lysed by repeat-edly freezing and thawing using lysis buffer containing0.6% ... receptor to cross-link withother TLR4 molecule since recent evidence indicates thatcross-linking is necessary for signal transduction [39].Cross-linking of receptors or receptor clustering by ... to increase the production of cytokines and generate of reac-tive oxygen species [1]. CS causes lung damage by oxida-tive stress either by itself or due to oxidants released byinflammatory...
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Báo cáo y học: "Heat shock protein 70 expression, keratin phosphorylation and Mallory body formation in hepatocytes from griseofulvin-intoxicated mice" pptx

... staining pattern in many hepa-tocytes and was also present in MBs (Fig. 2F).Phosphorylation of K8 S79, K8 S436 and K18 S33 during GF intoxicationCryosections of control and GF-treated C3H and ... HSP70i and IFs occurs only in a few hepatocytes. Since the hepa-tocytes, in which colocalization was observed, containedK8 pS79 or K8 pS436, HSP70i binding to IFs in these cellsmay be related to ... ofhepatocytes to the presence of a hepatotoxic agent. In the present study, we investigated the effect of chronicGF intoxication on hepatocytes from C3H and FVB/nmouse strains. We monitored...
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Báo cáo y học: "The ethics of characterizing difference: guiding principles on using racial categories in human genetics" potx

... iiddeeaallss?? In Science,Technology and Democracy.Edited byKleinman DL. New York: State Universityof New York Press; 2000:121-138.12. Fredrickson GM: Racism: A Short History.Princeton: Princeton ... sciences,law and medicine at Stanford Univer-sity convened over the past few years to engage in an extended interdisciplinarydialog. The initial meeting consistedof a two-day workshop in 2003 thatdeveloped ... Race and Ethnicity. This seminarseries, which continued for two years and culminated in a public conference,included invited experts and led to thepublication of a collection of essays [8].Our...
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Báo cáo hóa học: " Nutraceutical augmentation of circulating endothelial progenitor cells and hematopoietic stem cells in human subjects" potx

... to respond to injury signals, hypothetically resulting in enhanced healing.Granulocyte colony stimulating factor (G-CSF) and granulocyte-macrophage colony stimulating factor (GM-CSF) have been used in ... injury conditions [43], subsequent studieshave expanded this finding to situations of inflammation[44], and peripheral tissue injury [45-47]. Hematopoieticstem cells are being increasingly ... for6 days, results in a 58-fold increase in granulocytic pro-genitors and 24-fold increase in erythroid progenitors[62], which approximately correlated with CD34 counts[63]. Maintaining such...
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Báo cáo y học: "Folk medicine used to heal malaria in Calabria (southern Italy" pot

... “expert medicine”.Area of StudyIt is interesting to point out that in some peninsular and insular areas of Italy, despite all the drainage attemptsinitiated in the 16th century, malaria-associated ... [28]FabaceaeLupinus albus L. lupinu [56,58,62,68] To treat dermatitis in cattle Ep [50] Gingival anti-inflammatorySe [35]FagaceaeQuercus spp. cerza [55,58] Intestinal astringent,detoxifying, cicatrizingDriedbark ... still used to treatmalaria in hospitals and in general by 19th-century phy-sicians, before the introduction of quinine. This elementis very interesting; while the empir ical and magic reme-dies...
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Báo cáo y học: " Fetal death due to upper airway compromise complicated by thyroid storm in a mother with uncontrolled Graves’ disease: a case report" pps

... therapy, in mothers withthyroidectomy during pregnancy, in mothers with aprevious history of ablation therapy for Graves’ hyperthyr-oidism, and in the presence of fetal tachycardia and incidental ... with the signs and symptoms ofthyroid disease. Most clinicians are aware of other signs and symptoms of hyperthyroidism that indicate thyroiddisease and are not common in pregnancy, such asweight ... long-standing hyperthyroidism, large goiters, orsignificant exophthalmos and, if they remain untreated,they may worsen in the last trimester and toxemia, cardiacfailure, and even ‘thyroid storm’...
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Báo cáo y học: "A novel approach to modelling water transport and drug diffusion through the stratum corneum" pdf

... tensor:KKKKKKKKKKxxzzxy xzyx yy yzzx zy=⎡⎣⎢⎢⎢⎤⎦⎥⎥⎥.Considering the mass flux of fluid into an REV, and by further d efining R* Δx y z to be the volumetric inflow rate (with R* > 0 referring to ... and within studies linking permeability and solubility [6], SC geometry and permeability[9], and the depend ence of diffusivity and general SC barrier prop erties on permeabil-ity, corneocyte ... corneocytes contain pro-teins that can bind water molecules. Moreover, corneocytes are surrounded by a toughcellular envelope consisting o f covalently bound proteins and lipids, vastly differingfrom...
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