... with the real-time PCR assay
and in only 11 samples with a conventional PCR assay. The real-time assay using the TaqMan-system can therefore
be practically used for studying the epidemiology and ... NS1 gene. The
results of the real-time PCR assays were compared with
those of previously established, conventional PCR assays.
Materials and metho...
... the
metacarpophalangeal joints, and many of the small carpal
bones have irregular margins with indentations (for example, at
the attachment of ligaments), making discrimination between
normal anatomy and presence ... duration 7 years (range 4–22 years)), and three con-
trol individuals were female and one was male (median age 36
years (range 34–57 years)). All individuals...
... Medicine,
Animal Care and Use Committee. The rats were cared
for in accordance with the Guide for the Care and Use of
Laboratory Animals.
Statistical analysis
The results were recorded by the principal ... control and air leaks when the
authors performed recurrent thoracotomy for any
reason, and confirmed the efficacy of TachoSil® as an
anti-adhesive ag...
... approximately 2% of the with-
drawal cases, the cause of cessation was registered as both
treatment failure and AE; for these patients, the cause of with-
drawal was subsequently classified as an AE. Patients ... activity when comparing infliximab with
etanercept, and bias in the withdrawal of patients because of
treatment failure is therefore unlikely. Finally,...
... out the
bonding experiments and the cytotoxicity assay. JB carried out
the analysis of the extracellular matrix content. JF carried out
the analysis of the relaxation behaviour and participated ... determined for all
substances employed, that is, surface degrading agents and
cross-linkers, using the resazurin assay. Taking the favourable
cell vitality after treat...
... In
Table 1
Basic clinical data, details of arthropathy, serologic findings, and therapy
Case Age/gender Clinical presentation
and arthropathy
Spleen, mm ANA RF IgM, IU/mL ANCA CCP aCL HP Therapy
1 84/F ... Hanna Makuch-Łasica
4
,
Mirosław Majewski
4
, Katarzyna Michalak
5
, Robert Rupiński
6
, Krzysztof Warzocha
7
and
Renata Maryniak
1
1
Department of Pathomorphology, Institute...
... are very complex
and so far no sensitive methods are available for detection
of viral and human RNA/DNA in human feces, we have
initially tested assay sensitivity for nucleic acid isolation
and ... subjects with and without
anti-retroviral therapy
Ayan K Chakrabarti, Lori Caruso, Ming Ding, Chengli Shen,
William Buchanan, Phalguni Gupta, Charles R Rinaldo and Yue Chen*
A...
... consisting of viruses from Southeast Asia
(genotype I), Southeast Asia and the Americas (genotype
II), Thailand (genotype III) and Malaysia (Sylvatic) [10].
The 2009 isolate belonged to genotype I or the ... of
theseasoninJune2010.Thetwocaseswerehospita-
lised patients and underwent standard daily cl inical eva-
luation and physical examinations. The case history
forms of...
... pmol of forward primer F3 and reverse primer R3, 2.5 mM
dNTPs and 0.75U DNA Polymerase. The amplification was performed using a PCR thermal
cycler (TaKaRa) and was initiated by heating for 5 ... genome containing overlapping regions to verify.
F3: 5-TTAAAGACCCCCCACTTAAACCCTAAATGA-3′, and R3 :5′ -
AGTGGGGGGTCTTTAAGATTAAATTCTCTG-3′. (Figure 1).
Figure 1 Schematic diagram...
... CCATCTTCCTTTGAAGTCCA
TGEV-FIP Forward
Inner
45 CGAGGTCACTGTCACCAAAATT
TGATGTGACAAGATTTTATGGAG
TGEV-BIP Reverse
Inner
42 GGAGCAGTGCCAAGCATTAC
AAAATGCTAGACACAGATGGAA
TGEV-LF Forward
Loop
17 GGCTGAACTGCTTCTAG
TGEV-LB ... Fragment; New England Biolabs,
USA) with 2 μL total RNA as template. The amplifica-
tion was performed at 60°C in a laboratory water bath
(Kangle, China, 25°C~99°C, with...