Báo cáo y học: " Construction of Metabolically Biotinylated Adenovirus with Deleted Fiber Knob as Targeting Vector" ppsx

... 7:316http://www.virologyj.com/content/7/1/316Page 4 of 6SHOR T REPOR T Open Access Construction of Metabolically Biotinylated Adenovirus with Deleted Fiber Knob as Targeting VectorYanzheng Liu, Philippe Valadon, ... Ad.9RPSTCD-GFP(avidin) virus was able to suc-cessfully transduce RAEC when these cells were pre-incubated with chemically biotinylated J120 antibodybut not with biotinylated J310 or no antibody at all (Fig-ure ... fusion [10]. Also a tru ncatedform of PSTCD fused to the C-terminus of the fiber pro-tein can be efficiently biotinylated by human holocarbox-ylase synthetase presented in HEK-293 cells [11]....

Báo cáo y học: "Construction of a high-resolution genetic linkage map and comparative genome analysis for the reef-building coral Acropora millepora" ppt

... that thenumber of synteny blocks, and the number of markers in eachblock, could have arisen by chance.Without any statistical tests, a simple analysis of syntenycould be easily misinterpreted; ... comparison: assembly 1.0 for the cnidarian N. vecten-sis and assembly 1.0 for the placozoan T. adhaerens. Thereference yeast genome assembly of S. cerevisiae was alsoincluded as a representative ... compared with other eukaryo-tic genomes using tblastx (bit-scores ≥50) to identify regions of synteny (the conserved linkage of genes in differentgenomes). This analysis included high-quality assemblies...

Báo cáo y học: " Construction of doxycyline-dependent mini-HIV-1 variants for the development of a virotherapy against leukemias" pps

... correlatenicely with the replication capacity of the respectiveviruses. There was no decrease in the number of live cellswhen the HIV-rtTA virus was tested without dox, confirm-ing that the increase ... infectionFreshly isolated PBMC were stimulated for 2 days with PHA (5 μg/mL), washed twice with medium and mixed with SupT1 cells. The cell mixture was analyzed by FACSstaining for CD4 and CD8 as described ... Berlin, Ger-many) as the capture antibody and alkaline phosphatase-conjugated anti-p24 monoclonal antibody (EH12-AP) as the detection antibody. Detection was done with thelumiphos plus system (Lumigen,...

Báo cáo y học: " Construction of a polycystic ovarian syndrome (PCOS) pathway based on the interactions of PCOS-related proteins retrieved from bibliomic data" docx

... expression profiles in human ovaries from polycystic ovary syndrome [16] 135The molecular characteristics of PCOS defined by human ovary cDNA microarray [17] 119Molecular profiling of polycystic ovaries ... the heterogeneity of this abnormality,but it is associated with insulin resistance, hyperandrogenism, obesity and dyslipidaemia. The mainpurpose of this study was to identify possible candidate ... inflammatory condition of PCOS [19]. Labora-tory findings support this idea as the inhibition of lipoxy-genases was found to enhance the action of insulin in ratmodels of insulin resistance and type...

Báo cáo y học: "Construction of Gene Regulatory Networks using biclustering and Bayesian networks" docx

... the University of Science & Technology, Yemen. The authors would like to thank Prof Dana Pe'er, Columbia University; Dr Kevien Yip, Yale University and Prof G. Stolovitzky, IBM Computational ... significantly enriched if the p-value of any of GO category is lower than the preset threshold P-value. The Bivisu and k-means biclusters are strongly affected by this filtration as they contain ... Generally, comparing different biclustering algorithms is not straightforward as they differ in strategy, approach, time complexity, number of parameters and predictive capacity. They are strongly...

Báo cáo y học: "Construction of predictive promoter models on the example of antibacterial response of human epithelial cells" pdf

... combinations of mutually exclusive pairs (comple-mentary pairs). The idea of starting the analysis with a"seed" of sequences allows a very biology-driven way of initial filtering of information.To ... other. As it has been men-tioned before, the discovery of complementary pairsentails automatically the discovery of the correspondingsubsets of sequences. We analyzed the distribution of theconstituents ... identify and predict subsets of target genespotentially triggered by different regulatory pathways andthus possessing different regulatory modules. The meth-odology is easily applicable to any...

Báo cáo y học: "Treatment of proximal femur infections with antibiotic-loaded cement spacers"

... sensitivity profile of the particular causative organism. Both patients with no isolated organisms were treated with flucloxacillin and clindamycin, respectively. The systemic therapy was ended ... Gemany), each spacer was loaded with 4 g vancomy-cin (Fa. cell pharen GmbH, Hannover, Germany) per 80 g cement. In one case, 800 mg teicoplanin were used due to a vancomycin allergy of the ... 1). At the time of surgery, the mean age of the patients was 66 [52-77] years. After infection eradication, a prosthesis has been reimplanted in 8 cases. One pa-tient passed away due to an unclear...

Báo cáo y học: "Management of Critically Ill Patients with Severe Acute Respiratory Syndrome (SARS)"

... pathology of severe acute respiratory syndrome (SARS): a study of 8 autopsy cases from Singapore. Hum Pathol, 2003. 34:743-8. 51. Wang H.J., et al. Fatal aspergillosis in a patient with SARS who has ... particular, mortality was high among ICU patients: 28-day ICU mortality was variously reported to be 26-37% [42,64,65]. Older age, severity of illness, lymphocyte count, decreased steroid dose, ... Respiratory Distress Syndrome in Critically ill patients with severe acute respiratory syndrome. JAMA, 2003. 290:374-380. 40. Wong VWS., et al. Treatment of severe acute respiratory syndrome with...

Báo cáo Y học:Association of the thyrotropin receptor with calnexin, calreticulin and BiP Effects on the maturation of the receptor docx

... widevariety of unrelated nascent polypeptides. These peptidesusually show a high degree of hydrophobicity, which isconsistent with the likelihood that BiP interacts with sequences normally located within ... kindly supplied by A. J. Dorner and were as previously described [13], TSHR monoclonal antibody(A10) was kindly supplied by P. Banga (London, UK), as previously described [14].Cloning of CNX ... that at least 30% of the newlysynthesized proteins were degraded by the proteasome. Thedegradation rate varied, depending on the proteins. CFTR[21], tyrosinase [22] and thyroperoxidase [23]...

Báo cáo Y học: Selection of effective antisense oligodeoxynucleotides with a green ﬂuorescent protein-based assay Discovery of selective and potent inhibitors of glutathione S-transferase Mu expression doc

... scanningarrays [9]. As activity in cell-free assay may not alwayscorrelate with activity in cellular systems, we developed in thepresent study a novel cellular screening assay for theselection of effective ... (Table 2). Some of the ODNs (i.e. AS- 1, AS- 6, AS- 7, AS- 8, AS- 12 and AS- 15) were designed to inhibitselectively GSTM1 expression, whereas others (i.e. AS- 5, AS- 10, AS- 11, AS- 13 and AS- 14) were designed ... moiety is not affected, whereas the formation of theGSTM1-1 is. This would explain the higher isoformspeciﬁcity of AS- 5 and AS- 10 in the EGFP assay, comparedto the GST assay.In summary, we...

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