Báo cáo y học: "Detection of poliovirus by ICC/qPCR in concentrated water samples has greater sensitivity and is less costly using BGM cells in suspension as compared to monolayers" pot
... this article as: Balkin and Margolin: Detection of poliovirus by ICC/
qPCR in concentrated water samples has greater sensitivity and is less
costly using BGM cells in suspension as compared to ... in
concentrated water samples has greater
sensitivity and is less costly using BGM cells in
suspension as compared...
... Fugene and added
to each well. Following 42 to 48 hours of incubation, cells
were harvested and lysed. Activity of luciferase and β-galac-
tosidase was measured using the Luciferase Assay System
(Promega) ... assistance for the tail vein plasmid
DNA transfer assay. Research was conducted at the EO Lawrence Berkeley
National Laboratory and at the Joint Genome Institute. Thi...
... and
4-tert-butylphenol being bis-hydroxymethylated. The purity
of these compounds (> 96%) was checked by
1
HNMR
and HPLC.
Spectrophotometric determination of laccase activity
The activity of laccase was ... a series of oligomeric
polyphenol compounds, having increasing size and
decreasing solubility in water, was incubated with laccase.
The extent of substrate conversi...
... supported by
the existence (at least in Ets-1 and Ets-2) of an auto-
inhibitory domain w hich, in t he absence of a ccessory factors,
prevents Ets-1 binding to DNA [ 10,17]. W hen this d omain i s
removed, ... AP1 [12] and NFAT [16].
The necessity for the accessory factors is likely due to poor
DNA-binding af®nity of full-length Ets-1 rather than to
poor potential of...
... involvement of hyaluronic acid (HA) and pepti-dylarginine deiminase (PADI4) in the pathogenesis of rheumatoid arthritisProposed mechanism of involvement of hyaluronic acid (HA) and pepti-
dylarginine ... enhancing synovial thickness and inflamma-
tion during the pathogenesis of RA.
The principal plasma inhibitor of thrombin is antithrombin, a
single-chain 51 kDa glycopro...
... to
treat other inflammatory diseases including ankylosing spond-
ylitis, multiple sclerosis and inflammatory bowel disease, but
its efficacy in the therapy of these conditions is far less impres-
sive ... [23,25-27,47]. In addition to the regulatory
effect of adenosine in cytokine secretion, we have further
established that Th1 proinflammatory cytokine IL-1 and TNF-α
tre...
... CT
is disfavored by using ionising radiation and by the inability to
visualise soft tissue changes.
We recently published data on the reliability of erosion volume
measurements on CT and MRI in RA ... reliability, and a high level of sensitivity to change- dem-
onstrating that the OMERACT RAMRIS system, after proper
training and calibration of readers, appears su...
... proinflammatory cytokines
implicated as important mediators of arthritis. Increased levels of
these cytokines are found in the joints of rheumatoid arthritis
patients, and the cytokines trigger arthritis ... production of
proinflammatory IL-6 release demonstrated in our study.
HMGB1 is a potent inducer of several proinflammatory
cytokines. One of these cytokines, IL-1,...
... primarily infected cells, HIV indi-
rect killing of bystander cells through HIV proteins and/ or
by the proinflammatory state that is associated to ongoing
viral replication[22]. One subset of CD4+ ... HIV-1 infection and
could constantly shed HIV-1 and CD4
+
T cells into the intestinal lumen. To examine this hypothesis,
we monitored HIV-1 RNA/DNA and CD4 mRNA in fe...
... Pharmacy, San Jose,
CA) as previously reported [28-32]. The doxycycline was
freshly prepared and shipped within 24 hours. Doxycy-
cline has been shown to be a MMP inhibitor in a variety
of tissues ... [Immunohistochemical
study of inflammatory infiltrates in minor salivary glands in
Sjogren's syndrome and other autoimmune diseases]. Med
Clin (Barc) 1998, 111:681-686....